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Green preparation method for carbon dots used for biological imaging

A biological imaging and carbon dot technology, applied in chemical instruments and methods, luminescent materials, etc., can solve the problems of difficult preparation and application of carbon dots in large quantities, complex preparation methods, high energy consumption, etc., to ensure low toxicity and biocompatibility properties, simple preparation process, and simplified preparation process

Active Publication Date: 2018-08-21
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In short, the existing preparation methods are complicated, and the preparation process requires either high energy consumption or dangerous chemical reagents, which makes it difficult to prepare and apply carbon dots in large quantities.

Method used

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  • Green preparation method for carbon dots used for biological imaging
  • Green preparation method for carbon dots used for biological imaging
  • Green preparation method for carbon dots used for biological imaging

Examples

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Effect test

specific Embodiment approach 1

[0014] Embodiment 1: Weigh 1 g of 80-mesh coffee shell powder, soak and extract with 100 mL of 0.1 mol / L sodium hydroxide solution, filter, take the filtrate, and adjust the pH to 2-3 with 0.5 mol / L hydrochloric acid solution. Let the precipitate stand, filter and wash the precipitate with distilled water, dry the precipitate at 40°C and an absolute pressure of 0.01Mpa for 24 hours, and then dissolve and disperse it in 100mL water-methanol (1:1, v / v) mixed solution, at 12000 rpm Centrifuge at a speed of 30 minutes for 30 minutes, take the supernatant, and then dialyze for 24 hours with an 8000Da dialysis bag to retain larger particles, and the liquid outside the dialysis bag is the carbon dot dispersion.

specific Embodiment approach 2

[0015] Specific embodiment two: Weigh 1 g of larch bark powder of 40 mesh, soak and extract with 100 mL of methanol, centrifuge at a speed of 10,000 rpm for 20 minutes, take the supernatant, and then dialyze with 8000 Da dialysis bag for 12 hours to obtain carbon Dot dispersion.

specific Embodiment approach 3

[0016] Specific embodiment 3: Weigh 1 g of black tea, extract it with 100 mL of water through microwave assistance, and then centrifuge it at a speed of 5000 rpm for 15 minutes to obtain a carbon dot dispersion.

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Abstract

Carbon dots are a novel fluorescent material with high light stability and good biocompatibility and have great application potential in fields like biological imaging; however, conventional preparation methods for carbon dots are complicated, consume a large amount of energy consumption or need dangerous chemical reagents, so carbon dots are difficult to prepare and apply in large scale. The invention provides a green preparation method for carbon dots to overcome the above-mentioned disadvantages; and the green preparation method is greatly simplified, ensures low toxicity and good biocompatibility of carbon dots from the source, and allows prepared carbon dots to be more applicable to biological imaging. The green preparation method comprises the following steps: (1) preparation of rawmaterials: selecting raw materials rich in phenolic substances, wherein bulk raw materials should be pulverized and sieved; (2) extraction of the phenolic substances: extracting the prepared raw materials with a solvent to obtain a solution of the phenolic substances; and (III) separation of carbon dots: separating the solution of the phenolic substances by using physical methods such as centrifugation and dialysis to obtain the dispersion liquid of carbon dots. The dispersion liquid of carbon dots is subjected to pressure-reduced drying and dispersion with normal saline or the like before applied to biological imaging.

Description

technical field [0001] The invention belongs to the technical field of carbon quantum dots, and relates to a preparation method of carbon dots for biological imaging. Background technique [0002] Bioimaging is the cornerstone of the development of cell biology and biological research, and fluorescent materials play a vital role in it. Traditional fluorescent materials such as organic dyes and semiconductor quantum dots are mostly toxic and have poor biocompatibility. In recent years, carbon dots, a new type of fluorescent material with carbon as the main element and a diameter of less than 10 nm, have shown great promise in the fields of bioimaging and biosensors due to their excellent properties such as high photostability and good biocompatibility. It has great application potential and has received extensive attention. [0003] Carbon dots, also known as carbon quantum dots, are an emerging carbon nano-functional material with monodisperse and nearly quasi-spherical ge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/65
CPCC09K11/65
Inventor 李淑君陈志俊张馨月马灼明韩世岩任世学牛娜
Owner NORTHEAST FORESTRY UNIVERSITY
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