Green preparation method for carbon dots used for biological imaging

Abstract

Carbon dots are a novel fluorescent material with high light stability and good biocompatibility and have great application potential in fields like biological imaging; however, conventional preparation methods for carbon dots are complicated, consume a large amount of energy consumption or need dangerous chemical reagents, so carbon dots are difficult to prepare and apply in large scale. The invention provides a green preparation method for carbon dots to overcome the above-mentioned disadvantages; and the green preparation method is greatly simplified, ensures low toxicity and good biocompatibility of carbon dots from the source, and allows prepared carbon dots to be more applicable to biological imaging. The green preparation method comprises the following steps: (1) preparation of rawmaterials: selecting raw materials rich in phenolic substances, wherein bulk raw materials should be pulverized and sieved; (2) extraction of the phenolic substances: extracting the prepared raw materials with a solvent to obtain a solution of the phenolic substances; and (III) separation of carbon dots: separating the solution of the phenolic substances by using physical methods such as centrifugation and dialysis to obtain the dispersion liquid of carbon dots. The dispersion liquid of carbon dots is subjected to pressure-reduced drying and dispersion with normal saline or the like before applied to biological imaging.

Description

technical field [0001] The invention belongs to the technical field of carbon quantum dots, and relates to a preparation method of carbon dots for biological imaging. Background technique [0002] Bioimaging is the cornerstone of the development of cell biology and biological research, and fluorescent materials play a vital role in it. Traditional fluorescent materials such as organic dyes and semiconductor quantum dots are mostly toxic and have poor biocompatibility. In recent years, carbon dots, a new type of fluorescent material with carbon as the main element and a diameter of less than 10 nm, have shown great promise in the fields of bioimaging and biosensors due to their excellent properties such as high photostability and good biocompatibility. It has great application potential and has received extensive attention. [0003] Carbon dots, also known as carbon quantum dots, are an emerging carbon nano-functional material with monodisperse and nearly quasi-spherical ge...

AI Technical Summary

Problems solved by technology

[0005] In short, the existing preparation methods are complicated, and the preparation process requires either high energy consumption or dangerous chemical reagents, which makes it difficult to prepare and apply carbon dots in large quantities.

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