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Method for determining carbon and nitrogen stable isotopes in high carbon-to-nitrogen ratio sample

A stable isotope, high carbon-nitrogen ratio technology, applied in measuring devices, material analysis through electromagnetic means, instruments, etc., can solve the problems of high accuracy and achieve good reproducibility, simple operation, and high accuracy Effect

Active Publication Date: 2018-08-24
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the carbon-nitrogen ratio of the sample in this method has a high impact on the accuracy of the analysis results.

Method used

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  • Method for determining carbon and nitrogen stable isotopes in high carbon-to-nitrogen ratio sample
  • Method for determining carbon and nitrogen stable isotopes in high carbon-to-nitrogen ratio sample
  • Method for determining carbon and nitrogen stable isotopes in high carbon-to-nitrogen ratio sample

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method for determining carbon and nitrogen stable isotopes in a high carbon to nitrogen ratio sample, comprising the following steps:

[0020] a. The sample is dried at 80°C for 24 hours by soil or sediment, and ground through a 120-mesh sieve to obtain the sample to be tested.

[0021] b. Wrap the sample to be tested with a tin cup, and use IAEA-CH-6 (sucrose, δC13=-10.449‰), IAEA-CH-3 (cellulose, δC13=-24.724‰), NBS-18 ( Calcite, δC13=-5.014‰) is the standard product.

[0022] c. Wrap the sample to be tested with a tin cup, and use IAEA-N-1 (ammonium sulfate, δC15=0.4‰), IAEA-N-2 (ammonium sulfate, δC15=20.3‰) and IAEA-N0-3 in the δN15 detection (potassium nitrate, δC15=25.6‰) is the standard.

[0023] d. The conditions of the elemental analyzer are: carrier gas He pressure 50kPa, injector He purge flow rate 80ml / min, oxygen pressure 15 kPa, oxidation furnace 1020°C, reduction furnace 650°C, column oven 115°C, oxidation tube catalyst 25 grams of silver-plated cob...

Embodiment 2

[0029] a. Wetland soil sample δC13 detection, the sample was dried at 80°C for 24 hours, and ground through a 120-mesh sieve to obtain the sample to be tested. The carbon-nitrogen ratio of the sample was 12.85.

[0030] b. Wrap 5mg of the sample to be tested in a tin cup, and use IAEA-CH-6 (sucrose, δC13=-10.449‰), IAEA-CH-3 (cellulose, δC13=-24.724‰), NBS-18 (calcite, δC13 =-5.014‰) is the standard product.

[0031] c. The conditions of the elemental analyzer are: carrier gas He pressure 50kPa, injector He purge flow 80ml / min, oxygen pressure 15 kPa, oxidation furnace 1020°C, reduction furnace 650°C, column oven 115°C, oxidation tube catalyst 25 grams of silver-plated cobalt oxide and 25 grams of chromium chloride, and 50 grams of granular pure copper (φ 0.7mm) in the reduction tube.

[0032] d. The analysis conditions of the stable isotope mass spectrometer are: ion source vacuum 2×10 -6 mBar, ion source voltage 5KV, carrier gas He pressure 15psi, split gas He pressure 27...

Embodiment 3

[0047] comparative test

[0048]

[0049] In summary, the method of the present invention has the advantages of good repeatability, high efficiency, and high accuracy, and can meet the needs of CN isotope detection of high C / N samples.

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Abstract

The invention discloses a method for determining carbon and nitrogen stable isotopes in a high carbon-to-nitrogen ratio sample. The method comprises the following specific steps: a plant, soil or sediment sample is dried, ground through a sieve mesh, wrapped in a tin cup, and detected by elemental analyzer-stable isotope mass spectrometer. An excessive carbon dioxide signal in the high carbon-to-nitrogen ratio sample can be suppressed by the shunting effect of He on a sample gas, so that the ratio of carbon and nitrogen isotopes in the high carbon-to-nitrogen ratio sample can be rapid and accurately detected, the result is accurate, the reproducibility is good, and the method is efficient.

Description

[0001] This invention is funded by Tianjin Applied Basic and Frontier Technology Research Program (Project No. 15JCYBJC49200), Tianjin Normal University Development Fund (Project No. 52XK1505), Tianjin Normal University Municipal Key Laboratory Open Fund (Project No. 117-YF11700102). technical field [0002] The invention belongs to the technical field of stable isotope analysis, in particular to a method for detecting carbon and nitrogen isotopes in samples with a high carbon-to-nitrogen ratio. Background technique [0003] With the continuous development and improvement of carbon and nitrogen stable isotope analysis technology, its application in the environment and agriculture is becoming more and more extensive. Many scholars have used carbon and nitrogen stable isotopes to study the sources of organic matter and pollutants in oceans, lakes, wetlands, and farmland, to conduct environmental traces, and to reconstruct paleoclimate and paleovegetation through stable isotopes...

Claims

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Application Information

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IPC IPC(8): G01N27/62
CPCG01N27/62
Inventor 尚云涛王义东陈奕霖曹佳蕊杨曦李勇李军
Owner TIANJIN NORMAL UNIVERSITY
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