Proteus for reducing Fe(III) in clay mineral and application thereof in inhibition of expansibility of clay

A proteus and clay technology, applied in the direction of microorganism-based methods, bacteria, microorganisms, etc., can solve the problems of inability to utilize organic matter, narrow use of electron donors, and high environmental requirements

Active Publication Date: 2018-08-28
CHINA UNIV OF PETROLEUM (BEIJING)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these strains with iron reduction activity still have the following defects: 1) most strains can only survive in a strict anaerobic environment, which is not conducive to large-scale production and practical application; Use small molecular weight organic substances such as acetic acid and lactic acid as electron donors, and cannot use organic substances with large molecular weight such as glucose and sucrose, so the environmental requirements for practical applications are relatively high
[0005] Proteus is a ubiquitous strain in nature, but so far no acid-producing and aerogenic Proteus with iron-reducing activity has been reported

Method used

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  • Proteus for reducing Fe(III) in clay mineral and application thereof in inhibition of expansibility of clay
  • Proteus for reducing Fe(III) in clay mineral and application thereof in inhibition of expansibility of clay
  • Proteus for reducing Fe(III) in clay mineral and application thereof in inhibition of expansibility of clay

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Experimental program
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Effect test

Embodiment 1

[0051] Example 1. Screening, domestication, isolation and identification of the Fe(III)-reducing functional bacterium CA128 of the present invention

[0052] 1. Isolation, screening and domestication of Fe(III)-reducing fungus CA128

[0053] Screening and domestication method of Fe(III)-reducing bacteria CA128: according to the improved enrichment medium for iron-reducing bacteria (C 6 h 12 o 6 ·H 2 O 10g / L; NH 4 Cl 1.0g / L; KH 2 PO 4 0.27g / L; CaCl 2 0.05g / L; NaHCO 3 0.25 g / L; FeCl 3 ·6H 2 O 9.6g / L; pH=7.0-7.2) formula preparation medium.

[0054] The experimental steps are as follows:

[0055] (1) Pack the prepared culture medium into 100mL facultative anaerobic bottles, and the liquid volume in each anaerobic bottle is 80mL, and seal the anaerobic bottles that have been filled with nitrogen for 10 minutes, and place them in Sterilize in a high-temperature and high-pressure sterilizer, and control the sterilization conditions at 121°C for 15 minutes;

[0056] (...

Embodiment 2

[0068] Embodiment 2, Fe (III) reducing function bacterium CA128 growth curve and pH measurement in LB medium

[0069] The Fe(III)-reducing functional bacterium CA128 can understand the growth process of the bacterium, the occurrence time of each stage and the acid production ability by drawing the growth curve of the intermittent culture and the pH change of the medium.

[0070] The experimental steps are as follows:

[0071] (1) Prepare LB liquid culture medium, pack in 100mL facultative anaerobic bottles, each anaerobic bottle contains 80mL;

[0072] (2) After completing high temperature and high pressure sterilization, inoculate 5% seed liquid in an ultra-clean bench, and place it in a shaker at 35°C and 150rpm for cultivation;

[0073] (3) During the cultivation process, 4 mL of the culture solution was taken out by aseptic operation every 6 hours for centrifugation, the supernatant was removed and the bacteria were suspended with buffer, and then the absorbance and pH a...

Embodiment 3

[0074] Embodiment 3, different pH value, temperature and salinity are to the growth influence of Fe(III) reducing function bacterium CA128

[0075] (1) The influence of pH value on the growth of the bacteria:

[0076] ①Preparation of iron-reducing bacteria fermentation medium (sucrose 10g / L; NaCl 5g / L; NH 4 Cl 2g / L; KH 2 PO 4 1.4 g / L; K 2 HPO 4 3.7g / L; MgSO 4 ·7H 2 O 0.5g / L; CaCl 2 0.07g / L; NaCH 3 COOH, 10mM; yeast extract powder, 0.05g / L; trace element solution 1.0mL; pH=7.0-7.2), distributed in 100mL facultative anaerobic medium, the volume of each bottle is 80mL. Regulate the pH value in each bottle with 1mol / L hydrochloric acid and 1mol / L sodium hydroxide to be 5, 6, 7, 8 and 9 respectively;

[0077] ② Sterilize in a high-temperature and high-pressure sterilizer, add 5% of the inoculum to the seed solution, and then cultivate at 35°C and 150rpm for 36 hours;

[0078] ③During the cultivation process, take 4mL culture solution in a centrifuge tube through asepti...

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Abstract

The invention provides proteus for reducing Fe(III) in clay mineral and application thereof in inhibition of expansibility of clay. The proteus provided by the invention has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 15331 and the strain has iron reduction activity, facultative anaerobic performance and relatively wide utilization of electron donors; the Fe(III) in ferric citrate and montmorillonite also can be efficiently reduced; the proteus can be used for corroding the clay mineral in low-permeability oil reservoirs, and also can be used for reducing trivalent iron ions in expanded clay mineral, which is easily hydrated in the oil reservoirs, through the strain and a metabolic product thereof; changes of a lattice structure and phase transformation are carried out, so that the aims of improving the permeability, realizing channel drag reduction and improving the crude oil recovery ratio are realized.

Description

technical field [0001] The present invention relates to a kind of proteus and its application of inhibiting clay swelling, in particular to a kind of proteus which produces acid and gas and efficiently reduces Fe(III) in clay minerals, and the bacteria can reduce ferric citrate and montmorillonite The application of Fe(III) in rock minerals and inhibition of clay expansion belongs to the application of bioengineering technology in microbial dissolution of minerals, involving the dissolution of clay minerals in oil reservoirs, inhibition of hydration expansion of clay minerals and improvement of reservoirs Seepage and channel drag reduction technology field. Background technique [0002] Iron is the most abundant element on the earth and the fourth most abundant element in the crust. It is widely distributed in the surface sedimentary environment with Fe(II) and Fe(III) as the main valence states. According to statistics, the average content of iron in the depositional envir...

Claims

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Application Information

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IPC IPC(8): C12N1/20C09K8/582C12R1/37
CPCC09K8/582C09K2208/12C12N1/20C12N1/205C12R2001/37
Inventor 崔凯孙珊珊张忠智徐全数沙特刘小楠罗一菁张志勇
Owner CHINA UNIV OF PETROLEUM (BEIJING)
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