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Uses of Alcaligenes faecalis, Alcaligenes faecalis wz-2 and the degradation agent prepared therefrom and use thereof

A technology of Alcaligenes faecalis and WZ-2, which is applied in the field of Alcaligenes faecalis WZ-2 and its prepared degradation agent, which can solve the negative effects of diversity and community structure, soil ecosystem toxicity effects, endocrine interference effects, etc. problems, achieve good application prospects, simple preparation process, and low cost

Active Publication Date: 2019-05-10
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large number of studies have shown that tebuconazole has certain toxic effects on mammalian non-target organisms, such as causing zebrafish embryo deformities and disrupting endocrine effects; it has high risks to Italian bees and Trichogramma corn, and has certain cumulative toxicity effect, causing potential chronic poisoning
In addition, tebuconazole has certain negative effects on soil microbial diversity and community structure, and has potential toxic effects on soil ecosystem health.

Method used

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  • Uses of Alcaligenes faecalis, Alcaligenes faecalis wz-2 and the degradation agent prepared therefrom and use thereof
  • Uses of Alcaligenes faecalis, Alcaligenes faecalis wz-2 and the degradation agent prepared therefrom and use thereof
  • Uses of Alcaligenes faecalis, Alcaligenes faecalis wz-2 and the degradation agent prepared therefrom and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Screening and Identification of Tebuconazole Degrading Bacteria WZ-2

[0021] The present inventors isolated and screened tebuconazole-degrading bacteria by enrichment culture method. The environmental samples used to enrich tebuconazole were collected from the tebuconazole-contaminated soil in Banruo Village, Dianzi Town, Boxing County, Shandong Province. Take 10g of soil sample and add 100mL basal medium (the formula is: NH 4 NO 3 , 1.0g; K 2 HPO 4 , 1.5g; KH 2 PO 4 , 0.5g; NaCl, 0.5g; MgSO 4 , 0.2g; distilled water 1000mL, pH 7.0). At the same time, tebuconazole original drug mother liquor (acetone as solvent) was added to make the final mass concentration of tebuconazole 50 mg / L, and degrading microorganism enrichment culture was carried out at 30° C. and 150 r / min shaking table. After culturing for 7 days, 10% of the inoculum was inserted into the second batch of basal medium containing 100 mg / L tebuconazole. After culturing for 7 days under the same condi...

Embodiment 2

[0028] Degradation Effect of Degrading Bacteria WZ-2 on Tebuconazole

[0029] 1) Preparation of bacterial suspension: insert the purified strain WZ-2 into 100 mL of LB liquid medium (tryptone, 10 g; yeast extract, 5 g; NaCl, 10 g; distilled water 1000 mL, pH 7.0) culture at 35°C with shaking at 150r / min to the logarithmic phase, collect the bacteria by centrifugation at 5000rpm for 10min, wash the bacteria with phosphate buffer (pH7.0) for 3 times and resuspend, adjust the absorbance value OD600 =1.0 as the strain suspension.

[0030] 2) Determination of degradation performance: Inoculate 3 mL of bacteria into 100 mL of basal culture solution containing different concentrations of tebuconazole (50, 100, 200, 400, 600 mg / L), and use no bacteria as a control, and adjust the pH to 7.0 , with three replicates per treatment. Incubate at 30°C and 150rpm in a constant temperature shaker for 10 days, take samples regularly, and measure the degradation of tebuconazole by UPLC-MS / MS. ...

Embodiment 3

[0035] Effects of Temperature and pH on Degradation of Tebuconazole by Strain WZ-2

[0036] The effect of temperature on the degradation of tebuconazole by bacteria WZ-2: inoculate 3mL of bacteria into 100mL of basal culture solution containing tebuconazole (100mg / L), and use no bacteria as a control. , 25°C, 28°C, 30°C, 35°C and 40°C in a constant temperature shaker at 150r / min for 3 days, the degradation of tebuconazole was determined by UPLC-MS / MS.

[0037] The effect of pH on the degradation of tebuconazole by strain WZ-2: Inoculate 3mL of bacteria into 100mL culture solution containing 100mg / L of different pH (5.0, 6.0, 7.0, 8.0, 9.0) respectively, with no bacteria as a control, each Set of three repetitions. After culturing in a constant temperature shaker at 30°C and 150rpm for 3 days, the degradation of tebuconazole was determined by UPLC-MS / MS.

[0038] Experimental results such as Figure 4 and Figure 5 As shown, the degradation effect of bacteria WZ-2 on tebuco...

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Abstract

The invention relates to a strain of Alcaligenes faecalis WZ‑2, which is isolated from vegetable soil polluted by tebuconazole. The strain was deposited in the General Microorganism Center of the China Committee for the Collection of Microorganisms on January 25, 2018, with the preservation number CGMCC NO.15301. After the degrading bacteria WZ-2 provided by the present invention is made into a bacterial suspension, it can be applied to the degradation of tebuconazole in the soil by directly adding it to the soil, and can quickly degrade the residual tebuconazole in the soil, thereby reducing the concentration of tebuconazole. The ecological pressure on the soil environment plays a role in repairing tebuconazole-contaminated soil and protecting the ecological environment. The bacterial agent preparation process of the bacterial strain is simple, high in efficiency, low in cost, free of secondary pollution, and has good application prospects.

Description

technical field [0001] The invention belongs to the technical field of biodegradation, and relates to the use of Alcaligenes faecalis, Alcaligenes faecalis WZ-2, a degradation agent prepared therefrom and the use thereof. Background technique [0002] Tebuconazole, the chemical name is (RS)-1-(4-chlorophenyl)-4,4-dimethyl-3-(1H-1,2,4triazol-1-ylmethyl)penta- 3-alcohol, formula C 16 h 22 ClN 3 O, molecular weight 307.8. As a high-efficiency, broad-spectrum, systemic triazole fungicidal pesticide, it has been registered on more than 60 crops in more than 50 countries in the world, and is widely used to control rust, powdery mildew, root rot and other crops Foliar and fungal soil-borne diseases. Tebuconazole was registered in China in 1995, and it has become one of the main products in my country's fungicide market. [0003] With the extensive use of tebuconazole and its long stability, its degradation half-life in different soils ranges from a few days to hundreds of day...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B09C1/10C12N1/20C12N1/02C12R1/05
CPCB09C1/10C12N1/02C12N1/205C12R2001/05
Inventor 张清明孙彤李梦瑶董怀洋王彩霞
Owner QINGDAO AGRI UNIV
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