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Processing technology of composite freeze-dried powder of sex glands of apostichopus ja ponicus

A processing technology and freeze-dried powder technology, which is applied in the processing technology field of A. japonicus gonadal compound freeze-dried powder, can solve the problem that the supporting technology of A. japonicus industrial industry is not yet mature, the deep-processing of sea cucumber is in its infancy, and there is not much research on comprehensive utilization. and other problems, to achieve the effect of improving extraction rate and bioavailability, increasing microbial fermentation links, and reducing pollution

Inactive Publication Date: 2018-09-14
WENZHOU VOCATIONAL COLLEGE OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since sea cucumbers were successfully moved to the south in 2006, the farming industry of sea cucumbers in the south has gradually stabilized, and the processing technology has also continued to improve. However, the supporting technology of the sea cucumber processing industry is not yet mature, and the deep processing of sea cucumbers is still in its infancy.
[0003] The gonads of sea cucumbers are the general designation of eggs and essence of sea cucumbers. Studies have shown that gonads of sea cucumbers are rich in nutrients and also contain active ingredients such as polysaccharides and saponins. sales or reprocessing, but in the initial processing of southern sea cucumbers, the gonads of sea cucumbers are directly discarded as waste, which not only causes waste of resources, but also causes environmental pollution
Studies have shown that sea cucumber peptides produced by enzymatic hydrolysis of sea cucumbers have good activity. The content of sea cucumber gonad protein is high and contains many active ingredients. However, there are not many studies on the comprehensive utilization of sea cucumber gonads. Utilization helps to reduce pollution, increase the added value of by-products, and promote breeding income and efficiency

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The present embodiment provides the processing technology of japonicus gonad compound freeze-dried powder, comprising the following steps:

[0021] (1) Preliminary enzymatic hydrolysis: Beat the gonads of sea cucumbers, then add compound protease, the enzyme addition amount is 0.5mKat / g, and the enzymatic hydrolysis time is 6h. After the enzymatic hydrolysis, the temperature is raised to kill the enzyme. Adjust the pH to 7.1.

[0022] There are many kinds of proteins in the gonads of sea cucumbers, and the one-time enzymatic hydrolysis is not thorough, resulting in waste. Firstly, the present invention uses complex proteases to enzymolyze them initially, and partially enzymolyzes the proteins with large molecular weights.

[0023] (2) Acid hydrolysis: add an organic acid, the weight of the added organic acid is 5% of the weight of the sea cucumber gonad, and maintain at 40°C for 3 hours. The function of adding organic acid is mainly to further release the protein in th...

Embodiment 2

[0032] Example 2 Determination of Polypeptide Content in Enzymolysis Supernatant

[0033] The determination of the peptide content in the enzymatic hydrolyzate was carried out with reference to the literature (Lu Wei, Ren Guopu, Song Junmei. Determination method of peptide content in protein hydrolyzate[J]. Food Science, 2005,26(7):169-171), Gly -Gly-Tyr-Arg standard products are configured into 0.0, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2, 1.4, 1.6 and 1.8mg / ml standard solutions, respectively take 6ml of the standard solution into a 10ml centrifuge tube, add double shrinkage 2.4ml of urea reagent A and 1.6ml of biuret reagent B, mix well. Let it stand for 10 minutes, centrifuge at 2000r / min for 10 minutes, take the supernatant after centrifugation and measure the OD value under a 540nm spectrophotometer, draw the standard curve with the peptide concentration as the abscissa and the OD value as the ordinate, and the peptide content in the sample is passed The regression equation is cal...

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Abstract

The invention discloses a processing technology of composite freeze-dried powder of sex glands of apostichopus ja ponicus. The processing technology comprises the following steps of (1) performing primary enzymolysis; (2) performing acidolysis; (3) performing microorganism fermenting and degrading; (4) performing secondary enzymolysis; and (5) performing drying. According to the processing technology disclosed by the invention, the sex glands of the apostichopus ja ponicus are used for comprehensive utilization, so that reducing pollution is facilitated, added values of byproducts are increased, and income increasing and efficiency improving during breeding are facilitated. According to the processing technology disclosed by the invention, a link of microorganism fermentation is additionally set, and besides, the steps of mutually and alternately performing enzymolysis and performing acidolysis are adopted, so that extraction rate and the bioavailability of polypeptides in the sex glands of the apostichopus ja ponicus can be greatly improved increased.

Description

technical field [0001] The invention relates to a processing technology of a japonicus gonad compound freeze-dried powder, which belongs to the field of biotechnology. Background technique [0002] Apostichopus japonicus, also known as sea cucumber, gray thorn, etc., belongs to the phylum Echinodermata, the class Holothuroidea, the order Aspidochirotida, the family Stichopodidae, and the genus Apostichopus. ), which are widely distributed in the northern coastal areas. With the artificial breeding of sea cucumbers and breeding technology becoming more and more mature, the sea cucumber farming industry has developed rapidly throughout the country, becoming one of the industries with the highest output value and profit in my country's aquaculture industry, and has led to the formation of Improve the industrial chain of nursery, breeding and processing. Since sea cucumbers were successfully moved to the south in 2006, the farming industry of sea cucumbers in the south has gradua...

Claims

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Application Information

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IPC IPC(8): A23J1/04A23J3/34
CPCA23J1/04A23J3/341
Inventor 徐仰丽苏来金叶剑林胜利戴璐怡邹盈苏凤贤
Owner WENZHOU VOCATIONAL COLLEGE OF SCI & TECH