Manufacturing method for microorganism heat-insulation building material
A manufacturing method, technology of microorganisms, applied in the direction of microorganism-based methods, methods of using microorganisms, microorganisms, etc.
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Embodiment 1
[0088] A preparation method of microbial polystyrene particle insulation board is as follows:
[0089] (1) Select the mixed bacteria KJ01 with the function of mineralization deposition, and use liquid medium for inoculation and cultivation. The composition of the liquid medium is: 1 L of ultrapure water, 10 g of tryptone, 5 g of yeast extract, 10 g of sodium chloride, and adjust the pH to 7.0 with 5 mol / L NaOH. After the bacteria were inoculated into the liquid medium, they were placed in a constant temperature shaker, and incubated at 30° C. at a speed of 120 r / min for 24 hours to obtain a bacteria solution.
[0090] (2) Centrifuge the bacteria solution obtained in step (1) for 2 minutes to obtain the bacteria sludge for later use.
[0091] (3) Dilute the bacteria slime obtained in step (2) with distilled water until the OD600 value is 2.0 to obtain the bacteria liquid for later use.
[0092] (4) Mix the bacterial liquid obtained in step (3) with the expanded perlite powder...
Embodiment 2
[0099] A preparation method of microbial polystyrene particle insulation board is as follows:
[0100] (1) Select the mixed bacteria KJ01 with the function of mineralization deposition, and use liquid medium for inoculation and cultivation. The composition of the liquid medium is: 1 L of ultrapure water, 10 g of tryptone, 5 g of yeast extract, 10 g of sodium chloride, and adjust the pH to 7.0 with 5 mol / L NaOH. After the bacteria were inoculated into the liquid medium, they were placed in a constant temperature shaker, and incubated at 30° C. at a speed of 120 r / min for 24 hours to obtain a bacteria solution.
[0101] (2) Centrifuge the bacteria solution obtained in step (1) for 2 minutes to obtain the bacteria sludge for later use.
[0102] (3) Dilute the bacteria slime obtained in step (2) with distilled water until the OD600 value is 3.0 to obtain the bacteria liquid for later use.
[0103] (4) Mix the bacterial liquid obtained in step (3) with the expanded perlite powder...
Embodiment 3
[0110] A preparation method of microbial expanded perlite insulation board is as follows:
[0111] (1) Select the mixed bacteria KJ01 with the function of mineralization deposition, and use liquid medium for inoculation and cultivation. The composition of the liquid medium is: 1 L of ultrapure water, 10 g of tryptone, 5 g of yeast extract, 10 g of sodium chloride, and adjust the pH to 7.0 with 5 mol / L NaOH. After the bacteria were inoculated into the liquid medium, they were placed in a constant temperature shaker, and incubated at 30° C. at a speed of 120 r / min for 24 hours to obtain a bacteria solution.
[0112] (2) Centrifuge the bacteria solution obtained in step (1) for 2 minutes to obtain the bacteria sludge for later use.
[0113] (3) Dilute the bacteria slime obtained in step (2) with distilled water until the OD600 value is 0.9 to obtain the bacteria liquid for later use.
[0114] (4) Prepare nutrient solution for inducing microbial mineralization deposition. Use d...
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