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An enterobacteriaceae for preventing and controlling plant root cancer and its application

A technology of enterobacteria and microbial strains, applied in forestry and horticultural crop protection, agricultural fields

Active Publication Date: 2020-01-07
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the widespread occurrence of root cancer caused by A. tumefaciens on fruit trees and other plants, there is currently no registered biocontrol agent in my country

Method used

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  • An enterobacteriaceae for preventing and controlling plant root cancer and its application
  • An enterobacteriaceae for preventing and controlling plant root cancer and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1, Isolation and Identification of Enterobacter cowii 10DI2-2

[0047] 1. Screening of Enterobacter cowii strain 10DI2-2

[0048] Enterobacter cowii of the present invention (Enterobacter cowii) 10DI2-2 is to adopt dilution plate method and plate streaking method to separate and obtain from peach branch at the base of Zhengzhou Institute of Fruit Trees, Chinese Academy of Agricultural Sciences, and the separation method is:

[0049] (1) Isolation of Enterobacteriaceae

[0050] Agrobacterium tumefaciens TA-AT-2 was inoculated in YEB medium, cultured at 28°C and 200rpm for 16h, and the concentration of the bacteria solution was adjusted to 10 9 cfu / mL. The Agrobacterium tumefaciens suspension was inoculated into root cancer peach resistant variety "Northwest 13-1" (HAO et al., Systemic Acquired Resistance Induced by Agrobacterium tumefaciens in Peach and Differential Expression of PR1 Genes. HortScience[J], 2015, 50 (5): 666-672.) On the new shoots, select 5 tr...

Embodiment 2

[0074] Embodiment 2, the antibacterial activity assay of enterobacter cowii (Enterobacter cowii) 10DI2-2 and the preparation of bacterial agent

[0075] 1. Determination of antibacterial activity of Enterobacter cowii 10DI2-2

[0076] Agrobacterium tumefaciens ATCC 23308 and TA-AT-2 were inoculated on NA (recipe see above) slant respectively, and cultured at 28°C for 24h. Then add an appropriate amount of sterile water to the slope to prepare a suspension of pathogenic bacteria (ATCC 23308 T Mix with TA-AT-2 at 1:1 volume). Take 1ml bacterial suspension (10 8 cfu / ml) in a sterilized Petri dish, poured into YEB (recipe above) medium melted at 40-50°C, and shaken well to make a bacteria-carrying antagonist assay plate. Enterobacter cowii 10DI2-2 CGMCC No. 13535 obtained in Example 1 and cultured for 24 hours were picked and spotted on the antagonism assay plate. After culturing at 28°C for 1-2 days, observe the colony morphology and the formation of the inhibition zone, and ...

Embodiment 3

[0083] Embodiment 3, enterobacter cowii (Enterobacter cowii) 10DI2-2 bacterial strain is to the inhibition of Agrobacterium tumefaciens (A. tumefaciens) infecting sunflower to form root tumor nodules

[0084] Enterobacter cowii 10DI2-2 CGMCC No.13535 and A. tumefaciens bacterial suspension (ATCC 23308 T Mix with TA-AT-2 at a volume ratio of 1:1) at a volume ratio of 5:1 (V / V). In the mixture, the number of live bacteria of Enterobacter cowii 10DI2-2 CGMCC No.13535 and A. tumefaciens was 10 8 pieces / ml. The mixture was inoculated to the cotyledons of sunflower about 7 days after emergence, and the inoculum volume was 20 μL. For the specific method, refer to Wang Hongyan (reference: Wang Hongyan. Cherry and Cherry Blossom Root Cancer Pathogen and Biological Control Research: [Master's Thesis] China Agricultural University, 1998, p7.) method.

[0085] Replace the bacterial suspension of Enterobacter cowii 10DI2-2 CGMCC No.13535 with the same amount of sterile water, and the ba...

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Abstract

The invention discloses an enterobacteriaceae for preventing and treating plant root cancer and its application. The Enterobacter cowii provided by the present invention is specifically Enterobacter cowii 10DI2-2, and its preservation number in the General Microbiology Center of China Committee for Microorganism Culture Collection is CGMCC No. 13535. The strain is an endophytic bacterium in peach trees, and grows rapidly, and can colonize quickly and in large quantities in plant tissues, and has an ecological niche advantage; and the strain 10DI2‑2 can produce antibiotics, which can resist Agrobacterium tumefaciens (A. tumefaciens) Invasion has a certain effect; in addition, the strain can strongly inhibit A. tumefaciens (A. tumefaciens) from infecting susceptible plants sunflower to form nodules. This strain can replace or reduce the use of pesticides, protect plants and reduce damage from the environment and diseases. Pressure, has a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of protection of agricultural, forestry and horticultural crops, and relates to an enterobacteriaceae for preventing and treating plant root cancer and its application. Background technique [0002] Root cancer is a common bacterial disease caused by Agrobacterium tumefaciens. Agrobacterium tumefaciens has a wide host range and can infect 643 species of plants belonging to 93 families and 331 genera. It occurs commonly in some countries such as Europe, North America, South Africa and Asia. It occurs to varying degrees in peach cultivation areas in my country, with an incidence rate of 20% to 90% (Ma Deqin, Wang Huimin. Fruit tree root cancer and its biological control [J]. Chinese Fruit Tree, 1995, Issue 02). More serious root cancer occurred in Hebei, Shandong, Liaoning, Henan, Hubei, Jiangsu and other provinces. Peach tree seedlings suffer premature aging, plant dwarfing, and even death; mature trees in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P1/00C12R1/01
CPCA01N63/00C12N1/20C12N1/205C12R2001/01
Inventor 李世访李昱佳李茜张志想
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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