Method for rapidly and efficiently preparing high-purity ganglioside

A ganglioside, high-purity technology, applied in the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problems of long production cycle, low yield, low cost, etc., to improve production efficiency, Effects of improving separation efficiency and reducing the incidence of adverse reactions

Inactive Publication Date: 2018-09-18
海南益尔生物制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] 2015.09.18, it includes three steps: 1. Place the mammalian nervous tissue in an alcohol solvent, heat, stir and reflux to extract, then cool down the extract by stirring, and filter to obtain the precipitate I containing ganglioside components; 2. Precipitate Heat and dissolve substance I in organic solvent I, and maintain the heating temperature to carry out impurity adsorption under stirring conditions, filter the resulting solution and concentrate it with acetone for low-tempe

Method used

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Embodiment 1

[0019] The specific steps of a method for rapidly and efficiently preparing high-purity neurosides of the present invention are:

[0020] A method for rapidly and efficiently preparing high-purity gangliosides, the method comprises the following steps:

[0021] Step A: Extraction of pig brain extract; the extraction of crude pig brain extract includes the following steps:

[0022] A1: Extraction step: add the minced fresh pig brain and the extract into the extraction tank, the mass concentration ratio of the fresh pig brain and the extract is 1:3, stir for 20 minutes, let stand for stratification for 30 minutes, and then Press filtration, collect the supernatant and the filtrate after the pressure filtration, the extract includes chloroform and methanol, and the ratio of chloroform and methanol is 1:2;

[0023] A2: Layering step: add the filtrate collected in step A1 to drinking water with 20% of the volume of the filtrate, stir for 5 minutes, let stand for 10 hours, and coll...

Embodiment 2

[0032] A method for rapidly and efficiently preparing high-purity gangliosides, the method comprises the following steps:

[0033] Step A: Extraction of pig brain extract; the extraction of crude pig brain extract includes the following steps:

[0034] A1: Extraction step: add the minced fresh pig brain and the extract into the extraction tank, the mass concentration ratio of the fresh pig brain and the extract is 1:5, stir for 30 minutes, let stand for stratification for 40 minutes, and then Press filtration, collect supernatant and filtrate after pressure filtration, the extract includes chloroform and methanol, and the ratio of chloroform and methanol is 1:3;

[0035] A2: Layering step: add the filtrate collected in step A1 to drinking water with 30% of the volume of the filtrate, stir for 15 minutes, let stand for 15 hours, and collect the supernatant after layering;

[0036] A3: Saponification step: mix the supernatant in step A1 and the supernatant in step A2 to obtain ...

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Abstract

The invention relates to the field of biopharmaceuticals, in particular to a method for rapidly and efficiently preparing high-purity ganglioside. The method comprises the following specific steps ofextraction of a pig brain extract, GM1 silica gel chromatography purification and cationic resin purification, wherein the extraction of the pig brain extract comprises the following steps of extraction, stratification, saponification, resin chromatography, acid hydrolysis, acetone precipitation filtration and drying. Through extraction of the ganglioside with a heat extraction method, the extraction efficiency is improved greatly. The GM1 content is increased by more than twice through acid hydrolysis. Moreover, a high-pressure system is used for performing silica gel purification, thereby greatly shortening the purification time while improving the separation efficiency. Rapid and effective separation of GM1 and impurities is realized, on-line detection can be realized, subsequent point-plate detection is not needed, and the production efficiency is greatly improved. Moreover, substances affecting the clarity of an injection are removed through cationic resin chromatography, so thatthe occurrence rate of adverse reactions after treatment is reduced greatly.

Description

technical field [0001] The invention relates to the field of biopharmaceuticals, in particular to a method for rapidly and efficiently preparing high-purity gangliosides. Background technique [0002] Gangliosides are the most complex glycosphingolipids and exist in trace amounts in almost all tissues of vertebrates, but the highest content in mammalian brain and spinal cord tissues is about 0.3%. At present, the purification of GM1 is mainly based on atmospheric pressure silica gel column. For example, a Chinese patent discloses a method for extracting and separating gangliosides, application number: 201510598064.0, application date: [0003] 2015.09.18, it includes three steps: 1. The mammalian nerve tissue is placed in an alcohol solvent for heating, stirring and refluxing extraction, and then the extract is stirred and cooled to obtain a precipitate I containing gangliosides by filtration; 2. Precipitation The substance I is heated and dissolved in the organic solvent I...

Claims

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Application Information

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IPC IPC(8): C07H1/08C07H15/10
CPCC07H1/08C07H15/10
Inventor 楚秋平陈雷谢智远
Owner 海南益尔生物制药有限公司
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