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Bifidobacterium freeze drying preparation and special protection agent thereof

A technology for bifidobacteria and freeze-dried preparations is applied in the field of freeze-dried bacterial agents for bifidobacteria and their special protective agents, which can solve the problems of low freeze-drying survival rate and small number of viable bacteria, and achieve the improvement of freeze-drying survival rate. Effect

Inactive Publication Date: 2018-09-18
九江牧威利元科技中心(普通合伙)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of bifidobacterium freeze-dried preparation and its special protective agent, provide a kind of freeze-dried compound protective agent and its application method that can significantly improve the bifidobacterium freeze-dried survival rate and bacterial powder viable count, It solves the problems of low freeze-drying survival rate and small number of viable bacteria in the freeze-drying process of bifidobacterium powder, and improves the health care function of the powder

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: a kind of bifidobacterium lyophilized preparation, is prepared by the following method: the first, adopt the mode of liquid culture to cultivate bifidobacteria: after bifidobacterium is activated three times, press the inoculum size of 2% (V / V) Insert into MRS medium containing 0.5g / L cysteine ​​hydrochloride and cultivate for 18h; secondly, centrifuge the cultured bifidobacteria culture solution, 8000rpm, and centrifuge for 12min to obtain bifidobacterium sludge; 3. According to the ratio of 1g of bacteria slime: 5mL of the special protective agent of the freeze-dried bifidobacteria preparation, mix the bacteria and the special protective agent evenly, then pre-freeze in a low-temperature refrigerator at -40°C for 7 hours, and then put it into a freeze dryer Freeze-dry at a temperature of -55°C and a vacuum of 4-6 Pa for 20 hours to obtain the freeze-dried bifidobacterium preparation.

[0014] The special protective agent for freeze-dried preparations of ...

Embodiment 2

[0015] Embodiment 2: A kind of bifidobacterium lyophilized preparation, is prepared by the following method: the first, adopt the mode of liquid culture to cultivate bifidobacteria: after bifidobacterium is activated three times, press the inoculum size of 2% (V / V) Insert into MRS medium containing 0.5g / L cysteine ​​hydrochloride and cultivate for 18h; secondly, centrifuge the cultured bifidobacteria culture solution, 8000rpm, and centrifuge for 12min to obtain bifidobacterium sludge; 3. According to the ratio of 1g of bacteria slime: 5mL of the special protective agent of the freeze-dried bifidobacteria preparation, mix the bacteria and the special protective agent evenly, then pre-freeze in a low-temperature refrigerator at -40°C for 7 hours, and then put it into a freeze dryer Freeze-dry at a temperature of -55°C and a vacuum of 4-6 Pa for 20 hours to obtain the freeze-dried bifidobacterium preparation.

[0016] The special protective agent for bifidobacterium freeze-dried ...

Embodiment 3

[0017] Embodiment 3: a kind of bifidobacterium lyophilized preparation, prepare through following method: first, adopt the mode of liquid culture to cultivate bifidobacteria: after bifidobacterium is activated three times, press the inoculum size of 2% (V / V) Insert into MRS medium containing 0.5g / L cysteine ​​hydrochloride and cultivate for 18h; secondly, centrifuge the cultured bifidobacteria culture solution, 8000rpm, and centrifuge for 12min to obtain bifidobacterium sludge; 3. According to the ratio of 1g of bacteria slime: 5mL of the special protective agent of the freeze-dried bifidobacteria preparation, mix the bacteria and the special protective agent evenly, then pre-freeze in a low-temperature refrigerator at -40°C for 7 hours, and then put it into a freeze dryer Freeze-dry at a temperature of -55°C and a vacuum of 4-6 Pa for 20 hours to obtain the freeze-dried bifidobacterium preparation.

[0018]The special protective agent for freeze-dried preparations of bifidoba...

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Abstract

The invention belongs to the field of microorganisms, and relates to a bifidobacterium freeze drying preparation and a special protection agent thereof. The bifidobacterium freeze drying preparation is characterized by being obtained through freeze drying after the protection agent is added into the bifidobacterium thalli. The special protection agent for the bifidobacterium freeze drying preparation is characterized by being prepared from the following ingredients in parts by weight: 3 to 5 parts of xylo-oligosaccharides, 4 to 8 parts of lactose, 0.5 to 1.5 parts of polyethyleneglycol, 1.5 to3 parts of lactamic acid, 0.5 to 1 part of lysine, 3 to 5 parts of skimmed milk powder, 2 to 4 parts of stachyose, 0.1 to 0.5 part of Jilin ginseng oligopeptides, 0.4 to 0.6 part of sodium bicarbonate, 0.7 to 1.2 parts of sodium ascorbate and 80 to 100 parts of water.

Description

Technical field: [0001] The invention belongs to the field of biology, and in particular relates to a freeze-dried bifidobacterium bacterial agent and a special protective agent thereof. Background technique: [0002] Bifidobacterium (Bifidobacterium) is an anaerobic Gram-positive bacillus isolated from the feces of breast-fed infants by French scholar Tissier in 1899. The ends are often forked, hence the name Bifidobacterium. Bifidobacteria belong to the Actinomycetes Bifidobacterium genus, obligate anaerobic, Gram-positive, rod-shaped, non-spore-forming, and immobile. Because it can ferment sugar and produce a large amount of lactic acid, it is also called lactic acid bacteria. It can utilize glucose, lactose, galactose and fructose. Fructose is generally used as the carbon source and nitrogen as the only available nitrogen source. The strains of Bifidobacterium associated with humans are: Bifidobacterium longum, Bifidobacterium infantis, Bifidobacterium breve, Bifidoba...

Claims

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Application Information

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IPC IPC(8): C12N1/04C12R1/01
CPCC12N1/04
Inventor 张磊曾和华陈瑞
Owner 九江牧威利元科技中心(普通合伙)
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