Alkane and polycyclic aromatic hydrocarbon degrading compound bacterial agent and preparing method thereof
A technology of polycyclic aromatic hydrocarbons and compound bacterial agents, applied in chemical instruments and methods, biochemical equipment and methods, and methods based on microorganisms, etc., can solve the problems of secondary pollution, limited effect, high cost, etc., and achieve easy operation and implementation Low cost and the effect of making up for the deficiency
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[0025] The preparation method of the alkane and polycyclic aromatic hydrocarbon degrading composite bacterial agent of the present invention comprises the following steps:
[0026] Step 1: Single-bacteria fermentation
[0027] Adjust the pH value of the liquid fermentation medium A to 7.4-7.6, carry out aseptic treatment according to the conventional operation, insert the table-top Gordonia NR_61 according to the aseptic operation of 8%-10% of the liquid mass, keep the temperature at 25±1°C, and stir Speed 280-320rpm, ventilation 0.3-0.5M 3 / min, tank pressure 0.070-0.085MPa, after continuous fermentation for 75-82h, count live bacteria by hemocytometer, the number of bacteria reached 1.0×10 8 Fermentation was terminated when it was above individual / mL,
[0028] The formula of liquid fermentation medium A is as follows:
[0029] Sucrose 15-20g / L, peptone 1.8-2.0g / L, Na 2 HPO 4 1.5-2.0g / L, KH 2 PO 4 3.5-3.8g / L, (NH 4 ) 2 SO 4 4.0-4.5g / L, MgSO 4 ·7H 2 O 0.7-1.0g...
Embodiment 1
[0040] Step 1: Single-bacteria fermentation
[0041] Adjust the pH value of the liquid fermentation medium A to 7.4, carry out aseptic treatment according to the conventional operation, insert the table-top Gordonia NR_61 according to the aseptic operation of 8% liquid mass, keep the constant temperature at 25°C, the stirring speed at 320rpm, and the ventilation rate at 0.50M 3 / min, tank pressure 0.085MPa, after continuous fermentation for 80h, live bacteria were counted by hemocytometer, and the number of bacteria reached 1.0×10 8 Individual / mL to stop the fermentation;
[0042] Adjust the pH value of the liquid fermentation medium B to 7.2, carry out aseptic treatment according to the conventional operation, insert Pseudomonas stutzeri ZJ-7 according to the aseptic operation of 8% liquid mass, keep the temperature at 36°C, stir at 270rpm, and ventilate Volume 0.35M 3 / min, tank pressure 0.035MPa, after continuous fermentation for 70h, live bacteria were counted by hemocyt...
Embodiment 2
[0053] Step 1: Single-bacteria fermentation
[0054] Adjust the pH value of the liquid fermentation medium A to 7.5, carry out aseptic treatment according to the conventional operation, insert the table-top Gordonia NR_61 according to the aseptic operation of 10% of the liquid mass, keep the temperature at 26°C, stir at 300rpm, and ventilate at 0.40M 3 / min, tank pressure 0.075MPa, after continuous fermentation for 75h, live bacteria were counted by hemocytometer, and the number of bacteria reached 1.0×10 8 Fermentation was terminated when it was more than individual / mL;
[0055] Adjust the pH value of the liquid fermentation medium B to 7.0, carry out aseptic treatment according to the conventional operation, insert Pseudomonas stutzeri ZJ-7 according to the aseptic operation of 6% liquid mass, keep the temperature at 35°C, stir at 250rpm, and ventilate Volume 0.25M 3 / min, tank pressure 0.055MPa, after continuous fermentation for 72h, live bacteria were counted by hemocyto...
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