Two-photon fluorescence probe for highly selectively and sensitively detecting mercury

A technology of hydrogen atoms and compounds, applied in the field of two-photon fluorescent probes for highly selective and sensitive detection of mercury, can solve the problems of insufficient response speed, complex synthesis, and insufficient selectivity, and achieve high sensitivity, simple synthesis, and stability good sex effect

Inactive Publication Date: 2018-09-25
UNIV OF JINAN
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still some problems with the currently reported colorimetric and fluorescent probes, including insufficient selectivity, insufficient response speed, and complex synthesis.

Method used

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  • Two-photon fluorescence probe for highly selectively and sensitively detecting mercury
  • Two-photon fluorescence probe for highly selectively and sensitively detecting mercury
  • Two-photon fluorescence probe for highly selectively and sensitively detecting mercury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029]

[0030] Dissolve 300mg (1.47mmol) of 7-hydroxy-3-acetylcoumarin in 15mL of dichloromethane, then add 635mg (3.68mmol) of phenyl thiochloroformate and stir for 4 hours at room temperature, then use a rotary evaporator for rotary evaporation. A crude product is obtained. If a purer product is to be obtained, it can be passed through a column and then recrystallized in a mixed system of dichloromethane and petroleum ether (for example, v / v, 1:10) to obtain a pure product. 231mg of white pure product was obtained, the yield was 46%.

[0031] 1 H NMR verified the correctness of the product structure.

Embodiment 2

[0033] Figure 1a are different concentrations of Hg 2+ (0, 0.05, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 1, 1.5, 2μM) on the fluorescence spectrum of the probe (5μM); Figure 1b are different concentrations of Hg 2+ (0-2μM) vs. probe (5μM) fluorescence intensity and the linear relationship between the concentration.

[0034] It can be seen that the fluorescence changes before and after adding mercury ions are very obvious. Hg in the probe solution 2+ As the concentration increases, the fluorescence intensity gradually increases, and at (0-2μM)Hg 2+ Concentration range, Hg 2+ The concentration has a good linear relationship with the fluorescence intensity. It shows that the probe has a good response to mercury ions, and the probe can realize sensitive recognition of mercury ions. The standard limit of mercury ions in my country's drinking water standards is 0.001 mg / L. Therefore, the probe of the present invention can more accurately determine the content of mercury ions...

Embodiment 3

[0036] Figure 2a is the effect of mercury ions (5μM) and other different ion analytes (25μM) on the fluorescence spectrum of the probe (5μM). Figure 2b is the influence of other different ion analytes (25μM) on the fluorescence intensity of mercury ion (5μM) and probe (5μM) at 455nm wavelength respectively.

[0037]Analytes include: 1. Mercury ions, 2. Potassium ions, 3. Sodium ions, 4. Calcium ions, 5. Magnesium ions, 6. Zinc ions, 7. Aluminum ions, 8. Nickel ions, 9. Lead ions, 10 .Cadmium ion, 11. Copper ion, 12. Fluoride ion, 13. Chloride ion, 14. Bromide ion, 15. Carbonate ion, 16. Sulfate ion, 17. Nitrate ion, 18. Nitrite ion, except The concentration of mercury ions is 5 μM, and the concentration of other analyzed ions is 25 μM. All test conditions were completed in pure water, the probe used was the probe prepared in Example 1, and all spectra were measured at 25°C 60 minutes after the analyte was added. Specifically, pipette 50 μL of 1 mM probe stock solution int...

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Abstract

The invention relates to a two-photon fluorescence probe for highly selectively and sensitively detecting mercury. Specifically, the probe provided by the invention is a 7-hydroxyl-3-acetocoumarin compound and can be used as a mercury ion colorimetric fluorescence probe for detecting mercury ions. The probe can realize at least one of the following technical effects that the mercury ions are highly selectively identified, the mercury ions can be highly sensitively analyzed, the probe has a stable property and can be preserved and used for a long period, and the probe has a relatively strong anti-interference capability.

Description

technical field [0001] The invention relates to a 7-hydroxyl-3-acetylcoumarin compound used as a fluorescent probe for mercury ions, capable of highly selective and sensitive identification of mercury ions, or it can measure the concentration of mercury ions in a sample. Background technique [0002] Mercury is a metal element with severe physiological toxicity. Due to its persistence, easy migration and high bioaccumulation, it has become one of the most concerned environmental pollutants. Inorganic mercury ions in the environment can be converted by organisms into highly toxic methylmercury under certain conditions. Inorganic mercury mainly affects the kidneys, while methylmercury mainly damages the nervous system after entering the human body, especially the central nervous system. Both can be highly enriched in biological tissues through the food chain, thus causing great harm to humans and nature. Mercury poisoning will have an extremely bad impact on the whole societ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/16C09K11/06G01N21/64
CPCC07D311/16C09K11/06C09K2211/1007C09K2211/1088G01N21/643
Inventor 段庆霞吕小钰吴柳柳彩云耿卓凡朱宝存
Owner UNIV OF JINAN
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