Preparation and application of bacterial strain HCX-01 of verticillium dahliae capable of preventing and treating cotton verticillium wilt

Abstract

The invention discloses preparation and application of a bacterial strain HCX-01 of verticillium dahliae capable of preventing and treating cotton verticillium wilt. The bacterial strain disclosed bythe invention is bacillus safensis HCX-01, belongs to clostridium selected from a resistant cotton root soil strain in a cotton test field of Nanyang Normal University and has a preservation number ofCCTCC NO. M2018153. The bacterial strain HCX-01 provided by the invention is applied to not only the inhibition of verticillium dahliae, but also the prevention and the treatment of the cotton verticillium wilt. The preparation and the application of the bacterial strain HCX-01, disclosed by the invention, have the beneficial effects that the HCX-01 can be cultured on an artificial culture medium, is easy to obtain and culture and facilitates factory production, popularization and application; after being treated by fermentation liquor of the HCX-01 for 12 hours, a verticillium dahliae sporesuspension has a spore germination rate of only 9.7 percent, which has a better inhibiting effect than that with the blank 39.6 percent spore germination rate; the harm of the verticillium dahliae tocotton is effectively reduced; meanwhile, compared with a chemical agent, the HCX-01 solves the problems of environmental protection and the like.

Description

technical field [0001] The invention belongs to the technical field of biological control of plant diseases, and in particular relates to the preparation and application of a Verticillium dahlia strain HCX-01 capable of preventing and treating Verticillium dahliae. Background technique [0002] Verticillium wilt of cotton is one of the most important diseases in cotton production. Known as the "cancer" of cotton, the disease is a soil-borne vascular disease caused by Verticillium dahliae. The pathogenic bacteria have strong survival ability, and the sclerotia can survive for more than 10 years without a host in the soil , the pathogenic bacteria population is rich in genetic diversity and easy to mutate. In addition, the host range of Verticillium dahliae is very wide, foreign reports can damage 660 species of plants in 38 families, including 184 species of crops and 153 species of weeds. my country has identified at least 80 species of host plants in 20 families. Field cr...

AI Technical Summary

Problems solved by technology

Verticillium wilt not only reduces the yield of cotton, but also reduces the fiber quality, seed yield and seed oil production of cotton, which in turn causes serious economic losses

[0003] At present, although cotton verticillium wilt can be controlled through traditional methods such as crop rotation, soil improvement and chemical control, these methods have certain limitations and shortcomings, and their operability and adaptability are not very effective. In recent years, with the enhancement of people's environmental awareness, the application of chemical drug reagents has been gradually restricted. In addition to continuing to explore low toxicity, low residue, and high selectivity chemical pesticides, people pay more attention to the development of anti-bacterial agents as a supplement or alternatives

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