Environmental-friendly extraction purification method for algal polysaccharide sulfate

Abstract

The invention relates to the field of pharmacies and discloses an environmental-friendly extraction purification method for algal polysaccharide sulfate. The method comprises the following steps: (1)crushing dry kelp into small pieces, adding 16 times of first-grade purified water, heating till refluxing, and extracting for 5 hours; (2) concentrating an extract, adding 1% calcium chloride into aconcentrated liquid, and leaving to stand for one hour; (3) carrying out primary filtration on a medicinal liquid by using a rotary drum vacuum filtering machine, centrifuging, and filtering centrifuged supernate twice by using a kieselguhr filtration aid filtering system; (4) carrying out two-step ultrafiltration on filtrate; (5) concentrating a medicinal liquid, and decoloring with activated carbon; (6) adding 10% potassium chloride into a concentrated liquid, adding ethanol for a secondary time till an alcohol content is up to 70%, leaving to stand so as to obtain precipitate, centrifuging,and washing filter cakes with 95% ethanol; (7) carrying out vacuum drying on the filter cakes for 4 hours at 60 DEG C; (8) crushing a dried substance, so as to obtain algal polysaccharide sulfate ofmilky white or a light yellow powder. Tests show that the yield of the algal polysaccharide sulfate is increased to 1.2-1.3% from conventional 0.2-0.3%, and a remarkable effect can be achieved.

Description

technical field [0001] The invention relates to the field of pharmacy, namely an environmental protection extraction and purification method of fucoidan sulfate. Background technique [0002] In the prior art, fucoidan sulfate raw material (preparation trade name; Haikun Shenxi Capsules) is a second-class drug extracted from marine plant kelp (National Drug Standard YBZ00902003-2008), which is a kind of drug with unique structure and function. Sulfate ionic polysaccharides. It has the characteristics of low raw material cost, easy source and remarkable efficacy. Fucoidan sulfate is an active ingredient extracted from marine algae kelp, and is a rare sulfate ionic polysaccharide in nature. Through the cooperation between the Institute of Oceanology, Chinese Academy of Sciences and Jilin Province Huinan Changlong Biochemical Pharmaceutical Co., Ltd., fucoidan sulfate raw materials and Haikun Shenxi Capsules have obtained the national new drug certificate and realized industr...

AI Technical Summary

Problems solved by technology

[0004] The disadvantages are: using conventional kelp soaking and extraction, the fucose leaching rate can only reach about 0.2%, adding alkali and hydrochloric acid to purify and remove impurities, consuming a large amount of acid and alkali, increasing the difficulty of sewage treatment, and polluting the environment

Three times of plate and frame filter cloth filtration, low scale production efficiency and long time-consuming; 49kg diatomaceous earth vacuum drum filtration is not easy to separate and remove the unique alginate of marine organisms, and the acid-insoluble ash content of the finished product is often too high. Absorption has an effect

Since the active ingredient is polysaccharide, the molecular weight of the finished product is not easy to control

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