Environmental-friendly extraction purification method for algal polysaccharide sulfate

A fucoidan and purification method technology, applied in the field of pharmaceuticals, can solve the problems of difficult separation and removal of alginate, difficulty in controlling the molecular weight of finished products, and increase the difficulty of sewage treatment, etc., to achieve the reduction of acid-insoluble ash index, improvement of product purity, and remarkable process effect Effect

Inactive Publication Date: 2018-10-02
吉林省辉南长龙生化药业股份有限公司
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AI Technical Summary

Problems solved by technology

[0004] The disadvantages are: using conventional kelp soaking and extraction, the fucose leaching rate can only reach about 0.2%, adding alkali and hydrochloric acid to purify and remove impurities, consuming a large amount of acid and alkali, increasing the difficulty of sewage treatment, and polluting the environment
Three times of plate and frame filter cloth filtration, low scale production efficiency and long time-consuming; 49kg diatomaceous earth vacuum drum filtration is not easy to separate and remove the unique alginate of marine organisms, and the acid-insoluble ash content of the finished product is often too high. Absorption has an effect
Since the active ingredient is polysaccharide, the molecular weight of the finished product is not easy to control

Method used

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  • Environmental-friendly extraction purification method for algal polysaccharide sulfate

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Effect test

Embodiment 1

[0018] The environmental protection extraction and purification method of fucoidan sulfate is characterized in that the steps are as follows:

[0019] (1) Dried kelp is broken into pieces, add 16 times the amount of primary purified water, heat to reflux, and extract for 5 hours to obtain the extract.

[0020] (2) Concentrate the extract to half the volume, add 1% calcium chloride to the concentrate, and let it stand for 1 hour to obtain the liquid medicine.

[0021] (3) The medicinal liquid is firstly filtered with a rotary drum vacuum filter, and then the filtrate is centrifuged by a butterfly centrifuge. The centrifuged clear liquid is then filtered twice with a diatomite filter-assisted filter system. Select No. 300 coarse soil for diatomaceous earth, and select No. 20 fine soil with diatomaceous earth for the second filtration.

[0022] (4) Two-step ultrafiltration of the filtrate. The first step is to perform ultrafiltration and desalination through a 5000 molecular wei...

Embodiment 2

[0028] The environmental protection extraction and purification method of fucoidan sulfate is characterized in that the steps are as follows:

[0029] Break the dried kelp into small pieces, weigh 8000kg, divide into 16 parts, each 500kg, put them in 12500L extraction tanks, add 8000kg of first-class purified water to the extraction tanks, heat and reflux for 5 hours, filter, and extract Concentrate to half, add 1% calcium chloride to the concentrate, and let stand for 1 hour. The medicinal solution is filtered with a diatomaceous earth drum, the filtrate is passed through a DHC500 butterfly centrifuge, and the centrifuged supernatant is filtered twice with a CL-B-T10 filter aid filter system. The filtrate is first desalted through the 5000 molecular weight ultrafiltration concentration desalination system, and the 10000 molecular weight ultrafiltration concentration desalination system performs secondary ultrafiltration to screen out macromolecular polysaccharides and concent...

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Abstract

The invention relates to the field of pharmacies and discloses an environmental-friendly extraction purification method for algal polysaccharide sulfate. The method comprises the following steps: (1)crushing dry kelp into small pieces, adding 16 times of first-grade purified water, heating till refluxing, and extracting for 5 hours; (2) concentrating an extract, adding 1% calcium chloride into aconcentrated liquid, and leaving to stand for one hour; (3) carrying out primary filtration on a medicinal liquid by using a rotary drum vacuum filtering machine, centrifuging, and filtering centrifuged supernate twice by using a kieselguhr filtration aid filtering system; (4) carrying out two-step ultrafiltration on filtrate; (5) concentrating a medicinal liquid, and decoloring with activated carbon; (6) adding 10% potassium chloride into a concentrated liquid, adding ethanol for a secondary time till an alcohol content is up to 70%, leaving to stand so as to obtain precipitate, centrifuging,and washing filter cakes with 95% ethanol; (7) carrying out vacuum drying on the filter cakes for 4 hours at 60 DEG C; (8) crushing a dried substance, so as to obtain algal polysaccharide sulfate ofmilky white or a light yellow powder. Tests show that the yield of the algal polysaccharide sulfate is increased to 1.2-1.3% from conventional 0.2-0.3%, and a remarkable effect can be achieved.

Description

technical field [0001] The invention relates to the field of pharmacy, namely an environmental protection extraction and purification method of fucoidan sulfate. Background technique [0002] In the prior art, fucoidan sulfate raw material (preparation trade name; Haikun Shenxi Capsules) is a second-class drug extracted from marine plant kelp (National Drug Standard YBZ00902003-2008), which is a kind of drug with unique structure and function. Sulfate ionic polysaccharides. It has the characteristics of low raw material cost, easy source and remarkable efficacy. Fucoidan sulfate is an active ingredient extracted from marine algae kelp, and is a rare sulfate ionic polysaccharide in nature. Through the cooperation between the Institute of Oceanology, Chinese Academy of Sciences and Jilin Province Huinan Changlong Biochemical Pharmaceutical Co., Ltd., fucoidan sulfate raw materials and Haikun Shenxi Capsules have obtained the national new drug certificate and realized industr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003C08B37/0063
Inventor 张翼徐向夫李志凯李宁
Owner 吉林省辉南长龙生化药业股份有限公司
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