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Application of an isolated streptomyces nbf715 in the preparation of plant nematode insecticides

A technology of Streptomyces and Streptomyces fumigatus, applied in nematicides, plant growth regulators, applications, etc., can solve problems such as environmental pollution, nematode drug resistance, and crop phytotoxicity.

Active Publication Date: 2020-05-05
WUHAN CHUQIANG BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of these pesticides are destructive pesticides, which seriously affect the beneficial organisms in the soil. If they are used improperly, they will easily cause phytotoxicity to the stubble crops, and they require high pesticide application technology.
The use of non-fumigants for root irrigation can easily lead to nematode resistance, and at the same time pollute the environment and cause pesticide residues

Method used

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  • Application of an isolated streptomyces nbf715 in the preparation of plant nematode insecticides
  • Application of an isolated streptomyces nbf715 in the preparation of plant nematode insecticides
  • Application of an isolated streptomyces nbf715 in the preparation of plant nematode insecticides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Obtaining and Identification of Streptomyces enissocaesilis NBF715

[0023] An isolated strain of Streptomyces, isolated from the soil in Hefeng County, Enshi, belongs to Streptomyces enissocaesilis after 16s rDNA combined with physiological and biochemical identification, and was sent to the Chinese Type Culture Collection on July 7, 2017 Preserved by the center, classified and named: Streptomyces enissocaesilis (Streptomyces enissocaesilis) NBF715; deposit number is CCTCC NO: M2017414; location: Wuhan, China, Wuhan University.

[0024] In the present invention, Streptomyces enissocaesilis NBF715 may be referred to as NBF715 for short.

[0025] Morphological characteristics: The colony is round, with smooth edges and dense surface. The aerial hyphae are silver-gray, the base mycelium cork is yellow, and the colony is round, without pigment. The spore filaments are distributed in a straight chain, and the spores are oval or oval.

[0026] Physiological and biochemica...

Embodiment 2

[0034] Fermentation of Streptomyces enissocaesilis NBF715:

[0035] Preparation of Streptomyces enissocaesilis NBF715 seed solution: including activating the slant-preserved bacterial species on the ISP-2 plate, picking and inoculating it into 100 ml of seed medium, at 28°C, on a shaker at 160 rpm Cultivate for 4 days; the seed solution is obtained.

[0036] The formula of the seed culture medium is: 1L of culture medium includes 20g of mannitol, 10g of soybean peptone, 0.35g of potassium dihydrogen phosphate, 0.5g of calcium carbonate, 20 μL of soybean oil, and the rest is water.

[0037] Solid fermentation: Inoculate Streptomyces enissocaesilis (Streptomyces enissocaesilis) NBF715 seed solution in solid medium at 10%, the loading amount is 20%, and culture at 30°C for 10 days. The concentration of the culture obtained by this method is 5*10 10 Each spore / gram is the NBF715 bacterial agent, which is used in the following examples.

[0038] The solid fermentation medium is:...

Embodiment 3

[0042] Streptomyces enissocaesilis NBF715 in the control of tomato root-knot nematode

[0043] The test was carried out in a tomato continuous cropping greenhouse in September 2017. Treatment 1 was to apply NBF715 bacterial agent in holes when transplanting tomatoes, at a dosage of 3g / hole, and then cover with soil and water the roots. Treatment 2 was to irrigate NBF715 fermentation broth after transplanting, the dosage was 200mL / tree, and the control was not treated with chemicals. Each treatment of 4 plots, repeated 3 times, 20m per plot 2 . Under normal agricultural management, 15 tomato plants were randomly selected in each plot 14 weeks after spraying, and the disease level of the plants was investigated. Grading standards: Grade 0, 0-5% of roots have root knots; Grade 1, 6%-20 roots have root knots; Grade 2, 21%-45% of roots have root knots; Grade 3, 46%-70% of roots have root knots Root Knot; Grade 4, 71%-85% of roots have root knots; Grade 5, 86%-100% of roots have ...

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Abstract

The invention discloses an application of isolated streptomycete NBF715 in preparation of plant nematode insecticides. The streptomycete is found by an applicant to be useful against a variety of nematodes for the first time; compared with current use of synthetic pesticide control, the streptomycete NBF715 coming form the natural environment has the advantages of high efficiency, low toxicity, noenvironmental pollution, less proneness to drug resistance and the like, and broad application prospect is achieved.

Description

technical field [0001] The invention belongs to the technical field of crop control, and in particular relates to the application of an isolated streptomyces NBF715 in the preparation of plant nematode insecticides. Background technique [0002] Among the four major pathogens causing plant infectious diseases, plant parasitic nematodes are second only to fungi and surpass bacteria and viruses. Root-knot nematodes (Meloidogyne spp.) and cyst nematodes are important groups. [0003] Root-knot nematode occurs widely in the world, and its host range is wide, often causing large-scale crop yield reduction and causing huge losses to agricultural production. It is estimated that the annual crop loss caused by this worldwide is about 50 billion US dollars. Soybean cyst nematode is a soil-borne disease that seriously endangers soybean yield and quality. The occurrence of this disease is characterized by wide distribution, heavy damage, and many transmission routes. Generally, the y...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/28A01N43/22A01P5/00A01C1/06
CPCA01C1/06A01N43/22A01N63/00
Inventor 黄大野万中义曹春霞张亚妮刘晓艳王开梅姚经武朱志刚
Owner WUHAN CHUQIANG BIOLOGICAL TECH CO LTD