pH-responsive iron ferric oxide nanoparticles and its preparation method and application

A technology of ferric tetroxide and nanoparticles, which is applied in microcapsules, pharmaceutical formulations, photodynamic therapy and other directions, can solve the problems of easily causing self-rejection, large toxic and side effects, and unsatisfactory treatment effect of rheumatoid arthritis. The effect of good near-infrared light absorption properties, low toxicity, and good application prospects

Active Publication Date: 2020-06-26
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The problem to be solved by the present invention is to overcome the unsatisfactory treatment effect for rheumatoid arthritis in the prior art, the large toxic and side effects, and easy to cause self-rejection, but currently there is still a lack of a photothermal material that can be used as a photothermal treatment Photothermal agents are applied to the technical problems in the treatment of rheumatoid arthritis, providing a pH-responsive ferric iron tetroxide (Fe 3 o 4 -Hb) Nanoparticles and its preparation method and application

Method used

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  • pH-responsive iron ferric oxide nanoparticles and its preparation method and application
  • pH-responsive iron ferric oxide nanoparticles and its preparation method and application
  • pH-responsive iron ferric oxide nanoparticles and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Preparation and characterization of Fe3O4 nanoparticles:

[0032] Weigh 0.5g ferrous chloride tetrahydrate (FeCl 2 4H 2O), dissolve it in 25mL deionized water, stir in a magnetic stirrer (3000-3500r / min) to dissolve, after it is completely dissolved, quickly add 2.5mL concentrated ammonia water (NH 3 ·H 2 O) Oxidize at room temperature for 10 min with stirring, then add 5 mL of 0.02 mg / mL polyethyleneimine (PEI) alcohol solution, and continue stirring for 5 min. The mixed solution was placed in a reaction kettle and reacted at 120°C for 3h. After the reaction was completed, the product was collected with an external magnetic field to collect the black precipitate, and washed several times with ethanol and deionized water to obtain surface-modified amino group ferric iron tetroxide (Fe 3 o 4 ) nanoparticles; their morphology was characterized by transmission electron microscopy (JEM-100CXII). figure 1 Fe prepared as shown in A 3 o 4 Nanoparticles are uniform in s...

Embodiment 2

[0034] pH-responsive Fe 3 o 4 - Preparation of Hb nanoparticles (Hb layer thickness is 5nm) and characterization:

[0035] Take 0.5mL of 1mg / mL human hemoglobin (Hb) solution, add 2mM EDC and 5mM NHS, and react at room temperature for 15min. Then 1.4 µL of β-mercaptoethanol was added to terminate the EDC. Add 10 mL ferric oxide nanoparticle solution (1 mg / mL) and react at room temperature for 2 h. After the reaction, the product was repeatedly washed with an external magnetic field to obtain a pH-responsive Fe 3 o 4 - Hb nanoparticles. Its morphology was characterized by transmission electron microscope TEM (JEM-100CXII), such as figure 1 The shape shown in B is uniform and spherical, and the thickness of the Hb layer is 5 nm.

Embodiment 3

[0037] pH-responsive Fe 3 o 4 - Preparation of Hb nanoparticles (Hb layer thickness is 12nm) and characterization:

[0038] Take 1 mL of 1 mg / mL human hemoglobin (Hb) solution (the rest are the same as in Example 2). Its morphology was characterized by transmission electron microscope TEM (JEM-100CXII), such as figure 1 The morphology shown in C is uniform and spherical, and the thickness of the Hb layer is 9 nm.

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Abstract

The invention belongs to the field of nano-biomaterials, and discloses pH-responsive type ferroferric oxide nanoparticles as well as a preparation method and an application thereof. The preparation method comprises the following steps: firstly, preparing a Fe3O4 nanoparticle solution that the surface is modified by amino; and then, modifying ferroferric oxide nanoparticles via a natural high polymer material, namely human hemoglobin, so that the pH-responsive type ferroferric oxide nanoparticles are prepared. The surface of the material (the pH-responsive type ferroferric oxide nanoparticles)prepared by the invention is negatively charged under a normal physiological condition, so that in vivo circulation can be prolonged and a rheumatoid arthritis focus can be passively targeted easily;the nanoparticles can achieve charge reversal from negative charges to positive charges since an inflammatory joint part is slightly acid, so that combination to a negatively charged cell membrane becomes easier, and an cell extracting rate is improved; the ferroferric oxide nanoparticles are applicable to an efficient and low-toxicity thermal therapy mode; and a new thought is provided for the treatment of rheumatoid arthritis.

Description

technical field [0001] The invention belongs to the field of nano-biological materials, and relates to a pH-responsive ferric iron tetroxide (Fe 3 o 4 -Hb) Nanoparticles and their preparation methods and applications. Background technique [0002] Rheumatoid arthritis (RA) is a chronic autoimmune disease of unknown etiology, characterized by inflammatory cell infiltration in the synovial membrane, synovial hyperplasia, pannus formation, and continuous destruction of articular cartilage and bone. Occurs in small joints such as hands, wrists, and feet, and is mainly manifested as pain, swelling, and functional decline in the affected joints. In severe cases, it can lead to joint mobility impairment and deformity. The morbidity rate of this disease is high, and the 3-year morbidity rate without treatment is as high as more than 70%, which brings a huge burden to the family and society. [0003] At present, there are mainly the following methods for the treatment of rheumatoi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/62A61K41/00A61K9/51A61K47/64A61P19/02A61P29/00
CPCA61K9/5169A61K41/0057A61P19/02A61P29/00
Inventor 沈松戈延茹张盛昌戚学勇吴琳
Owner JIANGSU UNIV
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