Preparation method of liquid medical fertilizer for hydroponic flowers and medical fertilizer
A technology of medicinal fertilizer and liquid state, applied in the field of preparation of liquid medicinal fertilizer, can solve problems such as low N and P contents, and achieve the effect of saving procurement cost and being beneficial to environmental protection
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[0036] The preparation method of described biochemical fulvic acid is as follows:
[0037] a. Activate Aspergillus niger on the slant medium PDA, then shake the flask for 2-3 days at 20-25°C, the hyphae will evenly form balls, and obtain the shake flask seed solution C for later use; wherein the shake flask medium is glucose 8-10g / L, bran extract 25-27g / L;
[0038] b. Activate Geotrichum candidum on the slant medium PDA, and then shake the flask for 2-3 days at 25-27°C to obtain the shake flask seed liquid D; wherein, the shake flask medium is glucose 8-10g / L, bran Skin extract 25-27g / L;
[0039] c. After activating Bacillus pumilus, Penicillium and Pseudomonas on the slant medium NBY respectively, culture the shake flask at 32-35°C for 15-20h, and then mix according to 3:1:2 to obtain the shake flask seed liquid E; where the shake flask medium is glucose 32-35g / L, beef peptone 13-15g / L, yeast extract 1.0-1.5g / L, sodium chloride 5-6g / L, potassium dihydrogen phosphate 0.5-0.8...
Embodiment 1
[0046] Preparation of biochemical fulvic acid
[0047] a. Activate Aspergillus niger on the slant medium PDA, then shake the flask for 50 h at 25°C, the mycelium will evenly form balls, and obtain the shake flask seed liquid C for subsequent use; wherein, the shake flask culture medium contains glucose 10g / L, Bran extract 25g / L;
[0048] b. Activate Geotrichum candidum on the slant medium PDA, and then shake the flask at 27°C for 40 hours to obtain the shake flask seed solution D; wherein, the shake flask medium contains 10 g / L of glucose and 25 g / L of bran extract ;
[0049] c. After activating Bacillus pumilus, Penicillium and Pseudomonas on the slant medium NBY respectively, shake the flask for 20 hours at 32°C, and then mix at 3:1:2 to obtain the shake flask seed solution E; The bottle medium contains glucose 35g / L, beef peptone 15g / L, yeast extract 1.5g / L, sodium chloride 5g / L, potassium dihydrogen phosphate 0.7g / L, pH7.0;
[0050] d, configure the basic medium, wherei...
Embodiment 2
[0058] Preparation of biochemical fulvic acid
[0059] a. Activate Aspergillus niger on the slant medium PDA, then shake the flask for 70 hours at 20°C, the mycelium will evenly form balls, and obtain the shake flask seed liquid C for subsequent use; wherein, the shake flask medium contains glucose 8g / L, bran Skin extract 26g / L;
[0060] b. Activate Geotrichum candidum on the slant medium PDA, and then shake the flask at 35°C for 55 hours to obtain the shake flask seed liquid D; wherein, the shake flask medium contains 8 g / L of glucose and 26 g / L of bran extract ;
[0061] c. After respectively activating Bacillus pumilus, Penicillium and Pseudomonas on the slant medium NBY, culture the shake flask at 35°C for 16h, and then mix at 3:1:2 to obtain the shake flask seed solution E; The bottle medium contains 34g / L glucose, 14g / L beef peptone, 1.3g / L yeast extract, 5.5g / L sodium chloride, 0.7g / L potassium dihydrogen phosphate, and pH7.0;
[0062] d, configure the base medium, w...
PUM
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