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Novel method for detecting hydrogen peroxide on basis of MoS2 nanometer enzyme luminescence system

A technology of hydrogen peroxide and enzyme luminescence, which is applied in the direction of chemiluminescence/bioluminescence, and analysis through chemical reactions of materials, which can solve problems such as slow reaction, weak signal, and high detection limit

Inactive Publication Date: 2018-10-19
ZHONGBEI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the hydrogen peroxide detection system composed of MoS2 nano-type enzymes and TMB is based on the oxidation of TMB substrate and the discoloration (colorimetric detection), with weak signal, slow reaction and high detection limit. For this reason, further research is needed to develop Detection methods with better detection performance

Method used

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  • Novel method for detecting hydrogen peroxide on basis of MoS2 nanometer enzyme luminescence system
  • Novel method for detecting hydrogen peroxide on basis of MoS2 nanometer enzyme luminescence system

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Experimental program
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Embodiment

[0021] S1. Preparation of MoS2 two-dimensional nano-enzymes

[0022] Add 50 mg of MoS2 to 10 ml of aqueous solution containing 10 mg of bovine serum albumin (BSA), use a gun-type ultrasonic probe to sonicate for 6 hours under the condition of a power of 240 W, and then place it in a high-speed centrifuge at a speed of 5000 r / min for 45 minutes. Disperse for 10 minutes, then centrifuge at 15000r / min for 45 minutes, collect the supernatant, and store it at 4 degrees for later use;

[0023] S2, equipped with luminol solution and Tris-HCl buffer solution

[0024] equipped with luminol solution

[0025] Weigh 7.08mg of luminol reagent and dissolve it in 4ml of sodium hydroxide solution with a concentration of 0.1mol / L to prepare 0.1mol / L luminol mother solution, and store it in the dark at 4 degrees Celsius; use Tris-HCl The buffer solution is diluted to the required concentration.

[0026] Equipped with Tris-HCl buffer solution

[0027] Use an electronic balance to weigh 1 par...

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Abstract

The invention discloses a new method for detecting hydrogen peroxide on the basis of a MoS2 nanometer enzyme luminescence system. The new method comprises the following steps: S1, preparing MoS2 two-dimensional nanometer enzymes; S2, preparing 0.1 mol / L of a Rumino mother liquor and a Tris-HCI buffer solution; S3, diluting the prepared Rumino mother liquor with the Tris-HCI buffer solution to 5 mol / L of the Rumino mother liquor, then taking 40 micro-liters of the solution to be uniformly mixed with 40 micro-liters of hydrogen peroxide solution in a measuring cup, then adding 40 micro-liters of1 mol / L of MoS2 nanometer enzymes, reacting for 30 seconds, then putting the whole reaction system in a chemiluminescence measuring instrument, and collecting and recording an optical signal producedby reaction through a chemiluminescence instrument. The new method disclosed by the invention has the benefits that the intensity of the optical signal when the hydrogen peroxide is detected is enhanced, and the reaction time is shortened.

Description

technical field [0001] The invention relates to a method for detecting hydrogen peroxide, in particular to a novel method for detecting hydrogen peroxide based on a MoS2 nano-enzyme luminescence system. Background technique [0002] Hydrogen peroxide is an oxidant, which is a common metabolite in living organisms. Its detection and monitoring can tell whether life metabolism is normal. At the same time, one of the symptoms of certain diseases is abnormal hydrogen peroxide content, so peroxidation Hydrogen detection can provide scientific and objective evidence for monitoring the metabolism of living organisms and diagnosing diseases, and has very important research and development value. [0003] At present, it is recognized that the specific and sensitive means of detecting hydrogen peroxide is the catalase of organisms. However, due to the biological nature of catalase, its purification, storage and use conditions are harsh and the cost is high, so the demand for this kind...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76
CPCG01N21/76
Inventor 陈晓勇王新新熊继军杜栓丽贾平岗梁庭洪应平雷程李晨
Owner ZHONGBEI UNIV
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