Method for determinating glyphosate, aminomethylphosphonic acid and glufosinate pesticide residues in tea leaves

A technology for aminomethylphosphonic acid and pesticide residues, which can be used in measuring devices, instruments, scientific instruments, etc., and can solve the problems of high detection limit and unreachable

Inactive Publication Date: 2018-10-19
江苏安舜技术服务有限公司
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Problems solved by technology

[0002] The existing standard SN/T1923-2007 uses liquid chromatography-mass spectrometry to detect glyphosate residues in imported and exported foods, which is applicable to soybeans, wheat, rice, corn, sugar cane, oranges, perilla, chestnuts, tea detection and confirmation of residues of glyphosate (PM

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  • Method for determinating glyphosate, aminomethylphosphonic acid and glufosinate pesticide residues in tea leaves

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Embodiment Construction

[0013] The technical solutions in the embodiments of the present invention will be clearly and completely described below.

[0014] A method for determining glyphosate, aminomethylphosphonic acid and glufosinate-ammonium pesticide residues in tea leaves provided by the invention uses liquid chromatography tandem mass spectrometry, comprising the following steps:

[0015] 1) Weigh the sample and add 5-20mL methanol water to fully vortex, add 5-20mL dichloromethane, vortex, mix, ultrasonicate, take out, centrifuge, and take the supernatant to be purified;

[0016] 2) Put 2mL of the above supernatant on the activated CAX purification cartridge, elute with CAX eluent, collect in a 10ml round-bottomed plastic tube, blow with nitrogen to nearly dry, add 1mL of 1-5% boric acid buffer solution, and set aside;

[0017] 3) Take the spare solution, mix well, add 200 μL FMOC-Cl derivatization reagent, vortex mix for 1 min, derivatize overnight at room temperature, centrifuge at 4500 r / min...

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Abstract

The invention discloses a method for determinating glyphosate, aminomethylphosphonic acid and glufosinate pesticide residues in tea leaves. By a liquid chromatography tandem mass spectrometry, the method is characterized by comprising the following steps: 1), weighing a test sample, adding 5-20mL of methanol water, whirling thoroughly, adding 5-20mL of dichloromethane, whirling, uniformly mixing,performing ultrasonic treatment, taking out, centrifuging, and taking a supernatant for purification; 2), taking 2mL of the supernatant, eluting with a CAX eluent in an activated CAX purifying small column, collecting by using 10ml of a round-bottom plastic tube, performing nitrogen blowing till a nearly dry state, adding 1mL of a boric acid buffer solution with the concentration of 1-5% for lateruse; 3), taking the stock solution, uniformly mixing, adding 200[mu]L of an FMOC-Cl derivatization reagent, uniformly mixing by whirling for 1min, derivatizing overnight at normal temperature, centrifuging at the speed of 4500r/min for 5min, then filtering by using a 0.22[mu]m organic filtration membrane, and applying to a machine for detection. By the method, the detection limit is 0.010mg/kg.

Description

technical field [0001] The invention relates to a determination method, in particular to a determination method of glyphosate, aminomethylphosphonic acid and glufosinate-ammonium pesticide residues in tea leaves. Background technique [0002] The existing standard SN / T1923-2007 uses liquid chromatography-mass spectrometry to detect glyphosate residues in imported and exported foods, which is applicable to soybeans, wheat, rice, corn, sugar cane, oranges, perilla, chestnuts, tea detection and confirmation of residues of glyphosate (PMG) and its metabolite aminomethylphosphonic acid (AMPA) in ginseng, shrimp, fish, livestock and poultry meat, honey, spices, ginseng, but glyphosate and aminomethylphosphonic acid (AMPA) in tea The detection limit of phosphonic acid is too high, not reaching the organic detection standard of 0.010mg / kg. Contents of the invention [0003] In order to solve the problems in the above-mentioned background technology, the object of the present inve...

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 陈卫东
Owner 江苏安舜技术服务有限公司
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