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Solution for diluting horse radish peroxidase labeled antigen and preparation method thereof

A horseradish peroxidase and labeling technology, which is applied in measurement devices, instruments, scientific instruments and other directions to reduce non-specific binding

Inactive Publication Date: 2018-10-19
SUZHOU YOUJUN ENVIRONMENTAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In chemiluminescence immunoassay, the detection of antibodies by double-antigen sandwich method is a commonly used technique. In this technique, after the horseradish peroxidase-labeled antigen is formulated into a working solution, problems such as stability, linearity, and specificity are prone to occur.

Method used

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  • Solution for diluting horse radish peroxidase labeled antigen and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0015] A solution for dilute horseradish peroxidase labeling antigen, which is composed of the following components in parts by weight: 65 parts of dipotassium hydrogen phosphate, 32 parts of disodium edetate, 32 parts of citric acid, chlorine Potassium chloride 16 parts, sucrose 29 parts, magnesium chloride 15 parts, sodium hydroxide 6 parts, gentamicin sulfate 3 parts, bovine serum albumin 12 parts, sodium azide 2 parts, deionized water 290 parts.

[0016] A preparation method for diluting horseradish peroxidase-labeled antigen solution, comprising the following steps:

[0017] Step 1. Preheat deionized water to 33°C, then add dipotassium hydrogen phosphate, disodium edetate, citric acid, potassium chloride, sucrose, magnesium chloride, hydroxide Sodium, stirred until clear;

[0018] Step 2, adjusting the pH of the solution obtained in step 1 to 6.2;

[0019] Step 3: Stir the liquid obtained in Step 2 at a stirring speed of 120rpm, and add gentamicin sulfate, bovine serum ...

Embodiment 2

[0021] A solution for dilute horseradish peroxidase labeling antigen, which is composed of the following components in parts by weight: 55 parts of dipotassium hydrogen phosphate, 23 parts of disodium edetate, 25 parts of citric acid, chlorine 18 parts of potassium chloride, 26 parts of sucrose, 12 parts of magnesium chloride, 7 parts of sodium hydroxide, 3 parts of gentamicin sulfate, 15 parts of bovine serum albumin, 2 parts of sodium azide, and 260 parts of deionized water.

[0022] A preparation method for diluting horseradish peroxidase-labeled antigen solution, comprising the following steps:

[0023] Step 1. Preheat deionized water to 35°C, then add dipotassium hydrogen phosphate, disodium edetate, citric acid, potassium chloride, sucrose, magnesium chloride, hydroxide Sodium, stirred until clear;

[0024] Step 2, adjusting the pH of the solution obtained in step 1 to 6.3;

[0025] Step 3: Stir the solution obtained in Step 2 at a stirring speed of 120rpm, and add gen...

Embodiment 3

[0027] A solution for dilute horseradish peroxidase labeling antigen, which is composed of the following components in parts by weight: 65 parts of dipotassium hydrogen phosphate, 31 parts of disodium edetate, 29 parts of citric acid, chlorine 17 parts of potassium chloride, 28 parts of sucrose, 15 parts of magnesium chloride, 3 parts of sodium hydroxide, 2 parts of gentamicin sulfate, 15 parts of bovine serum albumin, 2 parts of sodium azide, and 320 parts of deionized water.

[0028] A preparation method for diluting horseradish peroxidase-labeled antigen solution, comprising the following steps:

[0029] Step 1. Preheat deionized water to 36°C, then add dipotassium hydrogen phosphate, disodium edetate, citric acid, potassium chloride, sucrose, magnesium chloride, and hydroxide at a stirring speed of 100 rpm. Sodium, stirred until clear;

[0030] Step 2, adjusting the pH of the solution obtained in step 1 to 6.2;

[0031] Step 3: Stir the solution obtained in Step 2 at a s...

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Abstract

The invention discloses a solution for diluting horse radish peroxidase labeled antigen and a preparation method thereof. The solution comprises the following components in parts by weight: 50 to 70 parts of dipotassium phosphate, 21 to 38 parts of ethylene diamine tetraacetic acid, 19 to 37 parts of citric acid, 11 to 21 parts of potassium chloride, 18 to 37 parts of saccharose, 9 to 19 parts ofmagnesium chloride, 2 to 9 parts of sodium hydroxide, 1 to 6 parts of gentamicin sulphate, 9 to 18 parts of bovine serum albumin, 1 to 4 parts of sodium azide, and 200 to 400 parts of deionized water.The solution is formed through synergistic effect among all the components, and is used for diluting the horse radish peroxidase labeled antigen into operating fluid, the performances of the operating fluid still meet specifications in the situation of long-term storage of the operating fluid, and nonspecific binding is reduced.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic reagents, in particular to a solution for diluting horseradish peroxidase-labeled antigens and a preparation method thereof. Background technique [0002] With the continuous advancement of medical technology, the window period of various diseases is continuously shortened, and the emergence of various signs and indicators makes it possible to detect various causes of disease early, so as to achieve early detection and early treatment. Among them, chemiluminescence technology is favored by medical practitioners and users because of its high sensitivity, easy operation, and fast detection speed. The cationic isozyme extracted from horseradish is widely used in chemiluminescence immunoassay because of its stability and high cost performance. In chemiluminescence immunoassay, it is a common technique to detect antibodies by the double-antigen sandwich method. In this technique, after the horseradi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/535
CPCG01N33/535
Inventor 凌云凌天阳金可心夏戊君
Owner SUZHOU YOUJUN ENVIRONMENTAL SCI & TECH