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Antibacterial peptide for killing oral cariogenic bacteria and inhibiting formation of dental plaque and application thereof

A technology of antimicrobial peptides and cariogenic bacteria, which is applied in the field of antimicrobial peptides for killing oral cariogenic bacteria and inhibiting the formation of dental plaque. The effect of overcoming the problem of drug resistance

Inactive Publication Date: 2018-10-26
ANHUI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem solved by the present invention is that traditional antibacterial agents cannot sustain antibacterial or antibacterial, and lack the ability to combine with teeth

Method used

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  • Antibacterial peptide for killing oral cariogenic bacteria and inhibiting formation of dental plaque and application thereof
  • Antibacterial peptide for killing oral cariogenic bacteria and inhibiting formation of dental plaque and application thereof
  • Antibacterial peptide for killing oral cariogenic bacteria and inhibiting formation of dental plaque and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Preparation of antimicrobial peptides, antimicrobial peptide A, antimicrobial peptide B, and antimicrobial peptide C of the amino acid sequence shown in SEQ ID NO:1:

[0046] (1) Weigh 2g of 2Cl resin, the degree of substitution is 0.2mmoL / g, and soak it in dichloromethane (DCM) for 15min in the reactor;

[0047] (2) Wash the resin with N,N-dimethylformamide (DMF) 3 times the volume of the resin, and then drain it, repeating this four times;

[0048] (3) Weigh 260 mg of 0.4mmoL of Fmoc-L-arginine (Fmoc-L-Arg(pbf)-OH) into the reactor, add 1.2mmoL of N,N-diisopropylethylamine (DIEA ), adding N,N-dimethylformamide (DMF) and dichloromethane (DCM) to react;

[0049](4) After 2 hours, cap the head with methanol for 0.5 hours, where the volume ratio of methanol to N,N-diisopropylethylamine (DIEA) is 1:1, and then use 3 times the volume of resin N,N-dimethyl Dimethyl formamide (DMF) washed four times, drained for later use;

[0050] (5) Add 20% piperidine to the reactor, wh...

Embodiment 2

[0065] Determination of the minimum inhibitory concentration of antimicrobial peptide A, antimicrobial peptide B, and antimicrobial peptide C:

[0066] (1) prepare an agar culture dish containing brain heart infusion broth (BHI);

[0067] (2) Prepare a number of circular filter paper sheets with a diameter of 6 mm, sterilize with high pressure steam, and dry;

[0068] (3) Cultivate Streptococcus mutans and adjust the concentration of the bacteria solution to 1×10 6 cfu / mL;

[0069] (4) Use a sterilized cotton swab to dip in the bacterial liquid and spread it evenly on the agar medium for 3 times, rotate the culture dish 60 degrees every time, and finally spread the cotton swab around the edge of the culture dish for one week.

[0070] (5) Add different concentrations of antimicrobial peptide A, antimicrobial peptide B, and antimicrobial peptide C to the filter paper, and the dropping amount is 20 μL. The filter paper treated with 20 μL sterile water is used as the control gr...

Embodiment 3

[0074] Effects of antimicrobial peptide A, antimicrobial peptide B, and antimicrobial peptide C on the growth of dental plaque:

[0075] (1) Preparation of enamel slices: several bovine incisors were obtained, sterilized with 3% sodium azide, and soft tissues were removed, rinsed with sterile water. The incisors were prepared into 5 mm × 5 mm × 2 mm enamel slices, which were polished successively with 600, 1200, and 2400-mesh sandpaper; then the enamel slices were ultrasonically cleaned with acetone, absolute ethanol, and sterile water for 5 minutes each; the treated enamel slices were After being sterilized by high pressure steam, place it in a refrigerator at 4°C and store it for later use;

[0076] (2) Cultivate Streptococcus mutans and prepare 10 6 cfu / mL bacteria solution, ready for use;

[0077] (3) Treatment of enamel slices: enamel slices were treated with sterile water, 80 μg / mL of antimicrobial peptides with the amino acid sequence shown in SEQ ID NO:1, 80 μg / mL of...

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Abstract

The invention discloses antibacterial peptide for killing oral cariogenic bacteria and inhibiting formation of dental plaque and application thereof, and relates to the field of microorganism application. The antibacterial peptide is provided based on the situation that a traditional antibacterial agent cannot continuously resist bacteria and is lack of ability of combining with teeth. The antibacterial peptide is formed by an amino acid sequence which is as shown in SEQ ID NO: 1 and a group having a function of combining with the surfaces of the teeth through polymerizing. The antibacterial peptide disclosed by the invention has the beneficial effects that the antibacterial peptide has the capability of combining with the surfaces of the teeth and can be applied to treatment and prevention of a dental caries disease.

Description

technical field [0001] The invention relates to the field of microbial application, in particular to an antibacterial peptide for killing oral cariogenic bacteria and inhibiting the formation of dental plaque and its application. Background technique [0002] Caries is the most common oral disease and has a significant impact on public health. Studies have shown that caries is a chronic progressive destructive disease of tooth hard tissue caused by a variety of bacterial infections, among which Streptococcus mutans is considered to be the most important pathogenic bacteria of caries. Streptococcus mutans can produce acid and can survive in an acidic environment for a long time. Streptococcus mutans infects the surface of the tooth and forms a lower acidic environment locally, which in turn causes the demineralization of the hard tissue of the tooth and the dissolution of organic matter, eventually forming caries . Under normal circumstances, Streptococcus mutans produces a...

Claims

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Application Information

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IPC IPC(8): A61K38/10A61K47/54A61P1/02A61P31/04A61K8/64A61Q11/00
CPCA61K8/64A61K38/10A61Q11/00A61K47/54A61K47/542A61P1/02A61P31/04
Inventor 曹颖李全利章礼玉戴若曦王晴晴张乐方泽辉吴乐平邵辉
Owner ANHUI MEDICAL UNIV
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