Guava enzyme liquid used as raw material for skincare product, and preparation method and application thereof
A technology of guava enzyme and pomegranate enzyme, applied in the field of fermented liquid, to achieve the effect of simple production process, short production cycle, and prevention of black spots and freckles
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Embodiment 1
[0027] (1) Raw material of guava enzyme liquid
[0028] The raw materials for preparing the guava enzyme liquid consist of the following components: 1000g of guava, 20g of quinoa, 10g of Dendrobium nobile, 10g of Dendrobium candidum, 20g of Yunnan gentian, 20g of lychee honey, and 5000g of cold boiled water.
[0029] (2) Preparation of Pichia pastoris and Lactobacillus acidophilus culture solution
[0030] The plate medium is PDA agar medium, containing 2.0% agar, and the pH is 6.0;
[0031] The shake flask seed medium is PDA liquid medium with a pH of 6.0.
[0032] The preparation method of the Pichia pastoris culture solution is as follows: inoculate the Pichia pastoris on the plate medium with an inoculation ring, cultivate it at 30°C for 18 hours until the colonies appear, pick the colonies from the plate medium and inoculate them on In the shake flask seed medium, Pichia pastoris was shaken and cultured at 30°C for 20 hours at a shaking speed of 200r / min to obtain a Pic...
Embodiment 2
[0042] (1) Raw material of guava enzyme liquid
[0043] The raw materials for preparing the guava enzyme liquid consist of the following components: 1100g guava, 30g quinoa, 20g Dendrobium nobile, 20g Dendrobium candidum, 30g Yunnan gentian, 30g litchi honey, and 5500g cold boiled water.
[0044] (2) Preparation of Pichia pastoris and Lactobacillus acidophilus culture solution
[0045] The plate medium is PDA agar medium, containing 2.0% agar, and the pH is 6.0;
[0046] The shake flask seed medium is PDA liquid medium with a pH of 6.0.
[0047] The preparation method of the Pichia pastoris culture solution is as follows: inoculate the Pichia pastoris on the plate medium with an inoculation ring, cultivate it at 30°C for 18 hours until the colonies appear, pick the colonies from the plate medium and inoculate them on In the shake flask seed medium, Pichia pastoris was shaken and cultured at 30°C for 20 hours at a shaking speed of 200r / min to obtain a Pichia pastoris culture ...
Embodiment 3
[0057] (1) Raw material of guava enzyme liquid
[0058] The raw materials for preparing the guava enzyme liquid consist of the following components: 1200g guava, 30g quinoa, 20g Dendrobium nobile, 20g Dendrobium candidum, 30g Yunnan gentian, 30g litchi honey, and 6000g cold boiled water.
[0059] (2) Preparation of Pichia pastoris and Lactobacillus acidophilus culture solution
[0060] The plate medium is PDA agar medium, containing 2.0% agar, and the pH is 6.0;
[0061] The shake flask seed medium is PDA liquid medium with a pH of 6.0.
[0062] The preparation method of the Pichia pastoris culture solution is as follows: inoculate the Pichia pastoris on the plate medium with an inoculation ring, cultivate it at 30°C for 18 hours until the colonies appear, pick the colonies from the plate medium and inoculate them on In the shake flask seed medium, Pichia pastoris was shaken and cultured at 30°C for 20 hours at a shaking speed of 200r / min to obtain a Pichia pastoris culture ...
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