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Method using bean dregs and bean curd yellow serofluid to prepare red yeast rice containing bacillus natto

A technology of natto bacteria and yellow syrup water, which is applied in the field of preparing red yeast rice containing natto bacteria, can solve the problems of high cost of culture medium, limited hypolipidemic effect, unfavorable large-scale production and application, etc. The effect of shortening fermentation time

Active Publication Date: 2018-11-02
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, red yeast rice containing Bacillus natto is mainly used in health food for reducing blood lipids. The preparation method of existing products is mainly made by simply mixing red yeast rice and natto, and providing carbon and nitrogen sources in the culture medium. The raw materials mainly include glucose and peptone, etc. This production method not only has a high cost of culture medium, but also has limited blood lipid-lowering effect, which is not conducive to large-scale production and application.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Monascus purple BNCC189220 ( Monasucs purpureus BNCC189220) liquid culture:

[0038] Liquid medium composition: Okara 4.5% (w / v), KH 2 PO 4 0.7% (w / v), CaCl 2 0.001% (w / v), the balance is tofu yellow slurry water, pH value is 3.5;

[0039] At the same time, set the basic medium as the control group, the composition is: glucose 4.5% (w / v), soybean peptone 8% (w / v), KH 2 PO 4 0.7% (w / v), CaCl 2 0.001% (w / v), the balance is distilled water, and then the two liquid cultures are sterilized at 115°C for 15 minutes, and after cooling to 35°C, 15mL of red yeast spore suspension is inoculated, and then at 32°C and After culturing at 180r / min for 72h, it was used as a liquid seed of red yeast rice.

[0040] After culturing for 72 hours, the biomass of the red yeast rice mycelium (marked as No. 2) in the control group reached 6.97g / ml, while the biomass of the red yeast rice mycelium (marked as No. 1) added with bean dregs and tofu yellow pulp water The amount can rea...

Embodiment 2

[0042] Bacillus subtilis ( Bacillus subtilis ) liquid culture:

[0043] Liquid medium composition: Okara 2% (w / v), KH 2 PO 4 0.7% (w / v), CaCl 2 0.001% (w / v), the balance is tofu yellow slurry water, the pH value is 6.5~7.5.

[0044] At the same time, set the basic medium as the control group, the composition is: glucose 2% (w / v), soybean peptone 10% (w / v), KH 2 PO 4 0.7% (w / v), CaCl 2 0.001% (w / v), the balance is distilled water, the pH value is 6.5~7.5. The volume of the 500mL triangular bottle is 200mL, and then the two liquid cultures are sterilized at 121°C for 20 minutes, and after cooling to 40°C , use an inoculation loop to inoculate three loops of Bacillus subtilis species that have been cultured for 20 hours from the nutrient agar, and then culture them at 35°C and 170r / min for 20 hours as a liquid seed of Bacillus natto for secondary fermentation.

[0045] After culturing for 20 hours, the OD of the liquid bacterial solution of Bacillus natto (referred to...

Embodiment 3

[0047] Solid state fermentation culture:

[0048] Weigh 500g of coarsely crushed soybeans, soak them in 1000g of nutrient solution (containing 15g / L bean dregs, and the balance is tofu yellow slurry) to absorb water for 4 hours, take it out and drain for 1 hour, and then fill the solid medium into 500mL In a glass bottle, the volume of each bottle is 180g, and then sterilized at 121°C for 40 minutes. After cooling to 35°C, each bottle is inoculated with 40mL of red yeast rice liquid seed added with bean dregs and tofu yellow syrup, and shaken well. Place it at 32°C for 6 days, then transfer it to 25°C for 20 days, then inactivate it at 121°C for 8 minutes, take it out and cool it to 35°C, add tofu water and bean dregs to each bottle of Bacillus natto liquid Sow 15ml, and ferment at 32°C for 72 hours. After the fermentation is finished, freeze and vacuum dry to obtain a red yeast rice product containing Bacillus natto.

[0049] It was determined that the activity of nattokina...

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PUM

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Abstract

The invention discloses a method using bean dregs and bean curd yellow serofluid to prepare red yeast rice containing bacillus natto. The method includes: inoculating red yeast rice into a red yeast rice liquid-state culture medium to perform strain expanding culture, and culturing under 30-33 DEG C for 50-92 hours to serve as the red yeast rice liquid-state strain; inoculating bacillus natto to abacillus natto liquid-state culture medium to perform strain expanding culture, and culturing under 30-35 DEG C for 10-60 hours to serve as the bacillus natto liquid-state strain; inoculating the redyeast rice liquid-state strain into a solid culture medium to perform fermentation, culturing under 30-33 DEG C for 4-6 days, culturing under 18-25 DEG C for 10-20 days, and deactivating; inoculatingthe bacillus natto liquid-state strain into the deactivated solid culture medium to perform secondary fermentation, and drying after the fermentation to obtain the red yeast rice containing the bacillus natto, wherein the bean dregs and bean curd yellow serofluid are added into the red yeast rice liquid-state culture medium, the bacillus natto liquid-state culture medium and the solid culture medium. Compared with a traditional red yeast rice liquid-state culture medium and a traditional bacillus natto liquid-state culture medium, the red yeast rice liquid-state culture medium and the bacillus natto liquid-state culture medium added with the bean curd yellow serofluid and the bean dregs can effectively promote the yield increasing of lovastatin and the enzyme activity of nattokinase.

Description

technical field [0001] The invention relates to the field of health food production, in particular to a method for preparing red yeast rice containing bacillus natto by using bean dregs and tofu yellow pulp water. Background technique [0002] Okara is a by-product of processing tofu, soy milk, and soy milk. It is rich in dietary fiber and protein, and has high nutritional value and health care functions. Practice has proved that eating bean dregs can reduce the cholesterol content in the blood, reduce the consumption of insulin by diabetics, and has the effects of lowering blood sugar, blood fat, weight loss, and preventing constipation. It has broad prospects for development and utilization. For every 1kg of tofu produced, about 1.2kg of fresh bean dregs are produced. It is speculated that my country produces approximately 2.8x10 9 kg bean dregs. At present, in the development and utilization of bean dregs in my country, except that some of them are used as feed for liv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/00A23L33/135C12P1/02C12P1/04C12R1/07C12R1/645
CPCA23L33/00A23L33/135C12P1/02C12P1/04A23V2002/00C12R2001/07C12N1/205C12R2001/645C12N1/145A23V2200/30Y02P60/87
Inventor 郝宁沈杨婷冯宇静郭格格李涛刘兆星欧阳平凯
Owner NANJING UNIV OF TECH
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