Method using bean dregs and bean curd yellow serofluid to prepare red yeast rice containing bacillus natto
A technology of natto bacteria and yellow syrup water, which is applied in the field of preparing red yeast rice containing natto bacteria, can solve the problems of high cost of culture medium, limited hypolipidemic effect, unfavorable large-scale production and application, etc. The effect of shortening fermentation time
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Embodiment 1
[0037] Monascus purple BNCC189220 ( Monasucs purpureus BNCC189220) liquid culture:
[0038] Liquid medium composition: Okara 4.5% (w / v), KH 2 PO 4 0.7% (w / v), CaCl 2 0.001% (w / v), the balance is tofu yellow slurry water, pH value is 3.5;
[0039] At the same time, set the basic medium as the control group, the composition is: glucose 4.5% (w / v), soybean peptone 8% (w / v), KH 2 PO 4 0.7% (w / v), CaCl 2 0.001% (w / v), the balance is distilled water, and then the two liquid cultures are sterilized at 115°C for 15 minutes, and after cooling to 35°C, 15mL of red yeast spore suspension is inoculated, and then at 32°C and After culturing at 180r / min for 72h, it was used as a liquid seed of red yeast rice.
[0040] After culturing for 72 hours, the biomass of the red yeast rice mycelium (marked as No. 2) in the control group reached 6.97g / ml, while the biomass of the red yeast rice mycelium (marked as No. 1) added with bean dregs and tofu yellow pulp water The amount can rea...
Embodiment 2
[0042] Bacillus subtilis ( Bacillus subtilis ) liquid culture:
[0043] Liquid medium composition: Okara 2% (w / v), KH 2 PO 4 0.7% (w / v), CaCl 2 0.001% (w / v), the balance is tofu yellow slurry water, the pH value is 6.5~7.5.
[0044] At the same time, set the basic medium as the control group, the composition is: glucose 2% (w / v), soybean peptone 10% (w / v), KH 2 PO 4 0.7% (w / v), CaCl 2 0.001% (w / v), the balance is distilled water, the pH value is 6.5~7.5. The volume of the 500mL triangular bottle is 200mL, and then the two liquid cultures are sterilized at 121°C for 20 minutes, and after cooling to 40°C , use an inoculation loop to inoculate three loops of Bacillus subtilis species that have been cultured for 20 hours from the nutrient agar, and then culture them at 35°C and 170r / min for 20 hours as a liquid seed of Bacillus natto for secondary fermentation.
[0045] After culturing for 20 hours, the OD of the liquid bacterial solution of Bacillus natto (referred to...
Embodiment 3
[0047] Solid state fermentation culture:
[0048] Weigh 500g of coarsely crushed soybeans, soak them in 1000g of nutrient solution (containing 15g / L bean dregs, and the balance is tofu yellow slurry) to absorb water for 4 hours, take it out and drain for 1 hour, and then fill the solid medium into 500mL In a glass bottle, the volume of each bottle is 180g, and then sterilized at 121°C for 40 minutes. After cooling to 35°C, each bottle is inoculated with 40mL of red yeast rice liquid seed added with bean dregs and tofu yellow syrup, and shaken well. Place it at 32°C for 6 days, then transfer it to 25°C for 20 days, then inactivate it at 121°C for 8 minutes, take it out and cool it to 35°C, add tofu water and bean dregs to each bottle of Bacillus natto liquid Sow 15ml, and ferment at 32°C for 72 hours. After the fermentation is finished, freeze and vacuum dry to obtain a red yeast rice product containing Bacillus natto.
[0049] It was determined that the activity of nattokina...
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