Method for extracting agaric polysaccharides

A technology of fungus polysaccharides and fungus, applied in the biological field, can solve the problems of long extraction time, complicated extraction process, and large amount of extraction solution, and achieve the effects of avoiding loss of activity, rapid extraction, and reduced concentration time

Inactive Publication Date: 2018-11-02
镇远县苗岭山珍有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the extraction process of this method is complicated, the extraction time i

Method used

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  • Method for extracting agaric polysaccharides

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Effect test

Embodiment 1

[0017] A method for extracting polysaccharides from fungus, the specific steps are:

[0018] Freeze-drying: freeze-dry 300 parts of fungus at -60℃ for 24 hours to obtain dry fungus;

[0019] Superfine grinding: Put the dry fungus into the airflow superfine grinding machine, using 8kg / m 2 Working pressure, superfine pulverization treatment under 10 conditions to obtain superfine fungus powder;

[0020] Enzymatic hydrolysis to break the wall: mix fungus superfine powder, sterile water, and cell wall enzyme at a mass ratio of 50:15:1, stir at 35℃ at a rate of 10r / min for 8h, and then at -60℃ , Freeze-drying for 24 hours to obtain a fungus freeze-dried powder; among them, the cell wall enzymes include pectinase, hemicellulase, and cellulase, and the mass ratio is 2:2:3.

[0021] Preparation of pre-extracted solution: Place 1500 parts of rose petals in a drying box, and dry them for 5 hours under the conditions of 0.5KPa and 45°C. After the gas is condensed, it becomes the rose cell liquid...

Embodiment 2

[0025] A method for extracting polysaccharides from fungus, the specific steps are:

[0026] Freeze-drying: freeze-dry 350 parts of fungus at -55°C for 24 hours to obtain dry fungus;

[0027] Superfine grinding: Put the dry fungus into the airflow superfine grinding machine, using 9kg / m 2 Working pressure, superfine pulverization treatment under 20Hz conditions to obtain superfine fungus powder;

[0028] Enzymatic hydrolysis and wall breaking: mix fungus superfine powder, sterile water, and cell wall enzyme at a mass ratio of 50:15:1, stir at a rate of 15r / min at 37°C for 9h, and then at -55°C , Freeze-drying for 24 hours to obtain agaric lyophilized powder; wherein the cell wall enzymes include pectinase, hemicellulase, and cellulase, and the mass ratio is 2:2:3.

[0029] Preparation of pre-extraction liquid: Place 1750 parts of rose petals in a drying box, and dry them for 6 hours under the conditions of 0.75KPa and 50°C. After the gas is condensed, it becomes the rose cell liquid; ...

Embodiment 3

[0033] A method for extracting polysaccharides from fungus, the specific steps are:

[0034] Freeze drying: freeze-dry 400 parts of fungus at -50°C for 24 hours to obtain dry fungus;

[0035] Superfine pulverization: put the dried fungus into the airflow superfine pulverizer, using 10kg / m 2 Working pressure, superfine pulverization treatment under 30Hz conditions to obtain superfine fungus powder;

[0036] Enzymatic hydrolysis and wall breaking: mix fungus superfine powder, sterile water, and cell wall enzyme at a mass ratio of 50:15:1, stir at 20r / min at 40℃ for 10h, and then at -50℃ , Freeze-drying for 24 hours to obtain agaric lyophilized powder; wherein the cell wall enzymes include pectinase, hemicellulase, and cellulase, and the mass ratio is 2:2:3.

[0037] Preparing the pre-extraction liquid: place 2000 parts of rose petals in a drying box, and dry them for 7 hours under the conditions of 1KPa and 55°C. After the gas is condensed, it becomes the rose cell liquid;

[0038] Micro...

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Abstract

The invention relates to the technical field of bio-extraction and in particular relates to a method for extracting agaric polysaccharides. The method comprises the following steps: performing freezedrying, superfine grinding, zymolytic wall breaking, pre-extract preparation, microwave extraction and concentrated drying, breaking structures between agaric cells and cell walls thereof, grinding tissue structures of the agaric, enlarging an enzymolysis contact surface, performing microwave extraction through rose cell sap and agaric freeze-dried powder, and rapidly separating out polysaccharideactive substances in agaric by virtue of beta-phenylethanol in the rose cell sap and volatile matters thereof. The used liquid-to-solid ratio is reduced, and the efficiency of extracting the agaric polysaccharides is increased.

Description

Technical field [0001] The invention relates to the field of biotechnology, in particular to a method for extracting polysaccharides from fungus. Background technique [0002] Fungus, belonging to the Basidiomycetes, Fungus, Fungus family. Dark brown color, soft taste, smooth taste, rich in nutrition, can be cooked or served cold, it is not only an ingredient for cooking delicious dishes, but also has medicinal uses such as nourishing blood and nourishing skin, making skin ruddy, preventing iron deficiency anemia, etc. effect. Among them, fungus polysaccharide is the main biologically active component in fungus, which has functions such as immune regulation, anti-tumor, anti-oxidation, lowering cholesterol, and regulating blood sugar. [0003] At present, most of the extraction techniques of fungus polysaccharides are enzymatic hydrolysis, supersound-assisted extraction, microwave-assisted extraction, superfine pulverization and other methods. There are also extraction methods th...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 陈虹霖陈彦达魏会英
Owner 镇远县苗岭山珍有限公司
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