Method for labeling heterologous protein expressed by insect protein or insect expression system

A protein and glycoprotein technology, applied in the field of labeling insect proteins or exogenous proteins expressed by insect expression systems, can solve the problems of long implementation period, unfavorable promotion, complicated operation, etc.
CN108728397AActive Publication Date: 2018-11-02INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
Publication Date
2018-11-02

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Abstract

The invention relates to a method for labeling heterologous protein expressed by insect protein or an insect expression system. The method comprises the following steps: doping azido-doped peracetylated N-acetylglucosamine into glycosyl of protein expressed by insects under the assistance of a metabolic pathway of the insects; subsequently, carrying out click chemistry reaction, and coupling a detachable marker to the protein, thereby realizing labeling of the protein from the insects. The method can be used for in-situ labeling of insect cells or labeling of the heterologous protein expressedby the insect expression system.
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Description

technical field

[0001] The present invention relates to a method for labeling proteins of insect origin by bioorthogonal reactions. The method can realize the in-situ labeling of the protein in the insect cell, and can also realize the labeling of the exogenous protein expressed by the insect baculovirus expression system. Background technique

[0002] Protein is the most abundant biomacromolecule in cells, and it participates in almost all life processes. Therefore, the study of the structure, function and interaction of proteins is an important task in the field of life sciences. In many cases, proteins need to be labeled for real-time and / or quantitative observation. Currently, the most commonly used methods for labeling proteins include fluorescent protein, radioactive isotopes, and non-specific modification of fluorescent groups to lysine or tyrosine in proteins through chemical reactions. However, these methods are often complicated to operate, and may also affect t...

Claims

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