Unlock instant, AI-driven research and patent intelligence for your innovation.

Nanogold-based NIMPLY logic gate and application thereof in visual detection of DNA

A nano-gold and logic gate technology, applied in the field of analysis and detection, can solve the problems of difficult integration of logic gates and complicated modification process, and achieve the effects of simple and fast preparation process, diversified signal reading, and high detection sensitivity

Inactive Publication Date: 2018-11-02
JINAN UNIVERSITY
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, many of the current DNA logic gates have the disadvantage of complex modification process, and there are limitations in the construction of multi-level logic gates based on unmodified gold nanoparticles, and the integration between different logic gates is also difficult.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nanogold-based NIMPLY logic gate and application thereof in visual detection of DNA
  • Nanogold-based NIMPLY logic gate and application thereof in visual detection of DNA
  • Nanogold-based NIMPLY logic gate and application thereof in visual detection of DNA

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Preparation of gold nanoparticles: All glassware used in the following process were soaked in aqua regia, ultrasonicated twice with distilled water, rinsed with ultrapure water, and dried. An aqueous solution of chloroauric acid (0.1 M, 0.25 mL) was mixed with 100 mL of ultrapure water and heated to boiling under reflux under magnetic stirring. Then trisodium citrate solution (1%, 1.5 mL) was quickly added into the mixed solution for reaction under stirring, and the stirring reaction was continued until wine-red nano-gold sol was generated. The reaction solution was gradually cooled to room temperature under stirring, and stored in the dark at 4° C., which could remain relatively stable for at least 20 days. The average particle diameter of the gold nanometer obtained by transmission electron microscopy is 18nm, and the concentration calculated according to Lambert-Beer's law is 0.30nM.

[0030] (2) Construction of NIMPLY logic gate: Add 50 μL of ultrapure water to...

Embodiment 2

[0032] (1) The preparation of nano gold is the same as in Example 1.

[0033] (2) Construction of NIMPLY logic gate: Add 25 μL of DNA with a concentration of 0.8 μg / mL to 25 μL of ultrapure water, react at room temperature for 5 minutes, then add to 450 μL of nano-gold, mix well, and immediately observe with the naked eye, the solution turns red. That is, when the input signal form is (0 / 1), the output signal is 0 (see figure 1 ).

Embodiment 3

[0035] (1) The preparation of nano gold is the same as in Example 1.

[0036] (2) Construction of NIMPLY logic gate: Add 25 μL of ultrapure water to 25 μL of palmatine with a concentration of 90 μM, react at room temperature for 5 minutes, then add to 450 μL of nano-gold, mix well, and immediately observe with the naked eye, the solution turns blue . That is, when the input signal is in the form of (1 / 0), the output signal is 1 (see figure 1 ).

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the technical fields of analysis and detection and discloses a nanogold-based NIMPLY logic gate and application thereof in the visual detection of DNA. An input signal 1 of the NIMPLY logic gate is palmatine, an input signal 2 is DNA, a signal converter is nanogold, and an output signal is the color of the nanogold. The application of the nanogold-based NIMPLY logic gate in the visual detection of DNA comprises the steps of respectively adding solutions with different concentrations, uniformly mixing, reacting at the room temperature, adding a signal converter solution, and judging the concentration of DNA according to the color of the nanogold of the output signal. The rapid visual concentration detection of DNA can be carried out by virtue of the NIMPLY logic gate according to the changes of the color of a nanogold sol and an ultraviolet absorption spectrum, so that the NIMPLY logic gate has the advantages of simplicity and convenience in operation, high detection sensitivity and the like and has relatively good application prospects in the fields of bioanalysis and the like.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and in particular relates to a NIMPLY logic gate based on nano-gold and the visual detection applied to DNA. Background technique [0002] Molecular logic gates are a concept similar to logic gates in digital circuits. In molecular logic gates, the input signal and output signal are two or more complex operations at the molecular or supramolecular level. DNA molecular logic gates use DNA molecules as operating elements, and use DNA to describe the input signals of logic gates to realize logic operations at the DNA molecular level. In recent years, various DNA logic gates that perform basic logic operations, such as NOT, AND, OR, NOR, and NAND, have been successively constructed. Among these logic gate systems, nanogold-based methods have attracted extensive attention due to their unique optical properties. When the gold nanoparticles are dispersed, the color is wine red, while t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33G01N21/78B82Y5/00B82Y15/00B82Y30/00
CPCB82Y5/00B82Y15/00B82Y30/00G01N21/33G01N21/78
Inventor 周海波江正瑾胡紫微袁凯松简敬一包芷君王梓涛
Owner JINAN UNIVERSITY