Method for culturing apple triploid germ plasm

A triploid and germplasm technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low emergence rate of hybrids, achieve good disinfection, facilitate rapid growth, and assist rapid growth Effect

Inactive Publication Date: 2018-11-06
SHANDONG INST OF POMOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the present invention provides a kind of method for cultivating apple triploid germplasm, to solve the problem that the emergence rate of hybrids is too low at present, in vitro test tube culture of apple hybrid seeds, the combination of proliferation and ploidy identification, directional The method of cultivating polyploids lays a technical foundation for the creation of new apple triploid varieties with excellent comprehensive traits

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Aseptic treatment of apple hybrid seeds

[0034] Select the mature fruit of 'Tetraploid Gala'×'Hanfu', remove the pulp, and take out the hybrid seeds, a total of 540 seeds, put the seeds into 100ml triangular flasks, and soak them in 70% (V / V) ethanol for 30s in turn , soak in 0.1% (M / V) mercuric chloride for 5 minutes, and rinse with sterile water for 3 times.

Embodiment 2

[0036] Hybrid Embryo Culture in Vitro

[0037] According to the conventional method, F14 medium, 1g / L activated carbon, hydrolyzed milk 500mg / L protein, 150mg / L ascorbic acid, 15g / L sucrose, 0.4mg / L6-BA, 0.2mg / LNAA, 6g / L agar were prepared into the culture medium , use 0.7% agar strips to solidify, adjust the pH of the medium to 5.8 with 1mol / L NaOH, divide the medium into test tubes, autoclave at 121°C for 20 minutes at 1 atmosphere, and then transfer to sterilized In the triangular flask of bacteria;

[0038] The hybrid embryos stripped of the seed coat were inoculated in an Erlenmeyer flask filled with culture medium, and cultured at a temperature of 25±2°C, a light intensity of 1500 lx, and a light cycle of 14 hours / day.

Embodiment 3

[0040] Ploidy Identification and Multiplication of Hybrid Seedlings

[0041] After 30 days of in vitro culture, hybrid embryos develop into full seedlings with a height of 10 cm. The leaves and root tips are collected under sterile conditions for ploidy identification, and the roots of the plants identified as triploid are removed and cut into 1.5 cm stem segments. Transfer to the subculture medium prepared by MS medium, 0.4mg / L6-BA, 0.2mg / LIAA, 30g / L sucrose, subculture once a month.

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Abstract

The invention discloses a method for culturing apple triploid germ plasm. The method comprises the steps: choosing a tetraploid gala apple*hanfu apple mature fruit, removing pulp, taking seeds out, disinfecting the seeds, peeling seed husks, inoculating to a culture medium to be cultured, culturing hybrid embryo in vitro for 30 days, performing ploidy determination on seedlings, removing root systems which are identified as triploid plant system, transferring into a subculture culture medium, performing subculture once about one month, inoculating to a rooting culture medium to grow roots wheneach plant system is multiplied to 10 plants or more, transplanting to a field nutritional bowl after rooting and performing general field management to obtain the apple triploid germ plasm. According to the culture method disclosed by the invention, polyploidy can be directionally cultured; the culture method has simpleness in operation; a polyploidy hybrid seedling rate can reach 90% or more; atriploid resource rate occupies 30% of seedling; the problems that apple polyploidy hybrid field general seeding cannot achieve seedling emergence or has a low seedling emergence rate are solved, andeffective technical support is provided for creating an apple polyploidy novel variety with excellent comprehensive characters.

Description

technical field [0001] The invention relates to the field of agricultural breeding, and relates to a method for cultivating triploid germplasm of fruit trees, in particular to a method for cultivating triploid germplasm of apples. Background technique [0002] Apple belongs to Rosaceae (Rosaceae) Malus, because of its strong ecological adaptability, high fruit nutritional value, good storability, and long supply cycle, it is widely cultivated all over the world. At present, the apple varieties cultivated in the world are mainly diploid varieties, but there are also triploid varieties with excellent performance traits. Apple polyploids generally have the characteristics of vigorous growth, large fruit with few or no seeds, high yield, strong adaptability and resistance to stress, etc., and can use asexual reproduction to fix their excellent traits and keep them stable without separation , long-term use in production. In view of the above advantages, the polyploidy breeding ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 何平李林光王海波常源升
Owner SHANDONG INST OF POMOLOGY
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