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Extraction method of adipose-derived stem cells

An adipose stem cell and extraction method technology, applied in the field of adipose stem cell extraction, can solve the problems of low cell adhesion rate and low cell extraction rate, and achieve the effects of improving extraction efficiency, stable chemical properties, and increasing extraction amount

Active Publication Date: 2018-11-06
CHENGDU QINGKE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the above-mentioned deficiencies in the prior art, the present invention provides a method for extracting adipose stem cells, which can effectively solve the problem of low cell attachment rate of adipose stem cells due to the existence of fat oil droplets in the extraction process. and the problem of low cell extraction rate

Method used

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  • Extraction method of adipose-derived stem cells
  • Extraction method of adipose-derived stem cells
  • Extraction method of adipose-derived stem cells

Examples

Experimental program
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Effect test

Embodiment 1

[0027] A method for extracting fat stem cells, comprising the following steps:

[0028] (1) collection of human adipose tissue;

[0029] (2) After fully mixing the collected adipose tissue with an equal volume of surfactant, centrifuge in a centrifuge at 1800rpm for 4min;

[0030] The surfactant used comprises the following components by weight: 0.3 part of hydroxyethyl starch 550, 0.5 part of lecithin, 0.1 part of polysorbate and 99.1 parts of PBS solution;

[0031] (3) Take the upper adipose tissue centrifuged in step (2), fully mix it with an equal volume of adipose tissue protective agent, and then centrifuge in a centrifuge at 1800 rpm for 4 minutes;

[0032] The adipose tissue protective agent used comprises the following components by weight: 0.1 part of trehalose, 0.01 part of reduced glutathione, 1 part of human albumin, 0.5 part of histidine, 0.02 part of sorbic acid, 0.1 part of VC and mixed Sugar electrolyte injection 93.5 parts;

[0033] (4) Inoculate the adipo...

Embodiment 2

[0037] A method for extracting fat stem cells, comprising the following steps:

[0038] (1) collection of human adipose tissue;

[0039] (2) After fully mixing the collected adipose tissue with an equal volume of surfactant, centrifuge in a centrifuge at 2200rpm for 6min;

[0040] The surfactant used comprises the following components by weight: 0.5 parts of hydroxyethyl starch 550, 2.5 parts of lecithin, 0.2 parts of polysorbate and 99.8 parts of PBS solution;

[0041] (3) Take the upper adipose tissue after centrifugation in step (2), fully mix it with an equal volume of adipose tissue protection agent, and then centrifuge in a centrifuge at 2200 rpm for 6 minutes;

[0042] The adipose tissue protective agent used comprises the following components in parts by weight: 0.3 part of trehalose, 0.1 part of reduced glutathione, 5 parts of human albumin, 1 part of histidine, 0.03 part of sorbic acid, 1 part of Vc and mixed Sugar electrolyte injection 99 parts;

[0043] (4) Inoc...

Embodiment 3

[0047] A method for extracting fat stem cells, comprising the following steps:

[0048] (1) collection of human adipose tissue;

[0049] (2) After fully mixing the collected adipose tissue with an equal volume of surfactant, centrifuge in a centrifuge at 2000rpm for 5min;

[0050] The surfactant used comprises the following components by weight: 0.3 part of hydroxyethyl starch 550, 1 part of lecithin, 0.1 part of polysorbate and 98.6 parts of PBS solution;

[0051] (3) Take the upper adipose tissue centrifuged in step (2), fully mix it with an equal volume of adipose tissue protection agent, and then centrifuge it in a centrifuge at 2000 rpm for 5 minutes;

[0052] The adipose tissue protective agent used comprises the following components in parts by weight: 0.2 part of trehalose, 0.05 part of reduced glutathione, 2 parts of human albumin, 1 part of histidine, 0.025 part of sorbic acid, 0.5 part of Vc and mixed Sugar electrolyte injection 96.2 parts;

[0053] (4) Inoculate...

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Abstract

The invention discloses an extraction method of adipose-derived stem cells. The extraction method comprises the following steps: acquiring adipose tissues, treating the adipose tissues with a surfactant, treating the adipose tissues with an adipose tissue protectant, performing inoculated culturing and the like. The used surfactant comprises the following components in parts by weight: 0.3 part ofhydroxyethyl starch 550, 1 part of lecithin. 0.1 part of polysorbate and 98.6 parts of a PBS solution. The used adipose tissue protectant comprises the following components in parts by weight: 0.2 part of trehalose, 0.05 part of reduced glutathione, 2 parts of human albumin, 1 part of histidine, 0.025 part of sorbic acid, 0.5 part of VC, and 96.2 parts of a carbohydrate and electrolyte injection.The extraction method can effectively solve the problems that in the extraction process of the adipose-derived stem cells, due to the existence of fat droplets, the cell attachment rate is low and the cell extraction rate is low.

Description

technical field [0001] The invention relates to the technical field of adipose stem cell extraction, in particular to a method for adipose stem cell extraction. Background technique [0002] Adipose tissue-derived stem cells (ADSCs) are a type of stem cells that can self-renew and reproduce, and have the multilineage differentiation potential of general stem cells and the stable multigenerational proliferation ability in vitro. Not only can it differentiate into adipocytes, osteoblasts, chondrocytes, muscle cells, nerve cells, etc. under the action of different inducing factors, but it can also secrete a large number of multi-type cytokines and promote angiogenesis and apoptosis factors. And it can act on fibroblasts through paracrine, promote the secretion of type I, type III collagen and fibronectin, promote collagen synthesis, and play anti-inflammatory, anti-oxidation, anti-aging, damage repair and other functions. Adipose tissue is abundant, easy to obtain, and has no ...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
CPCC12N5/0667C12N2509/00
Inventor 高雪华海泉
Owner CHENGDU QINGKE BIOTECH
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