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Method for measuring honeysuckle active ingredient by adopting HPLC

A technology of active components and honeysuckle, applied in the field of determination of active components of honeysuckle, can solve rare problems and achieve high recovery rate

Inactive Publication Date: 2018-11-16
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Researchers generally detect many types of single chemical components, but it is rare to detect multiple active components in honeysuckle simultaneously.

Method used

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  • Method for measuring honeysuckle active ingredient by adopting HPLC
  • Method for measuring honeysuckle active ingredient by adopting HPLC
  • Method for measuring honeysuckle active ingredient by adopting HPLC

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Preparation of 50% methanol solution of honeysuckle

[0037] After the honeysuckle powder is purified, weigh 1.000g of honeysuckle powder, put it in a 50mL conical flask with a stopper, add 50mL of 50% methanol, weigh it, ultrasonically treat it (power 600W, frequency 40KHZ, temperature 40°C) for 30min, and let it cool , Weigh the weight, make up the lost weight with 50% methanol, shake well, filter, set aside, take the continued filtrate and pass through a 0.22 μm organic filter membrane to obtain the product. Six parallel samples were prepared.

[0038] Preparation of reference solution

[0039] Accurately weigh 2.18mg neochlorogenic acid, 1.05mg cryptochlorogenic acid, 4.04mg rutin, 2.42mg luteolin, 1.53mg isochlorogenic acid B, put them into a 250mL brown volumetric flask, and dilute to volume with 50% methanol solution , dissolved, and mixed to obtain the first reference substance solution; respectively weigh 6.16mg of chlorogenic acid, 0.97mg of isochlorogenic aci...

Embodiment 2

[0048] A kind of method adopting HPLC method to measure honeysuckle active component, comprises the steps:

[0049] 1) Specific test

[0050] Under chromatographic conditions, 10 μL of honeysuckle 50% methanol solution and reference substance solution were injected into the high-performance liquid chromatograph for detection, and the results are shown in figure 1 (a) and (b); by figure 1 It can be seen that the resolution of adjacent chromatographic peaks of the two solutions is greater than 1.5, and at the corresponding retention time of each active component of the reference substance, each active component of the sample also has corresponding chromatographic peaks to appear; The active components in are separable, refer to the Pharmacopoeia of the People's Republic of China, 2010, Appendix 37.

[0051] Calculation formula of resolution R: R=2(t R2 -t R1 ) / (W1+W2); where, t R2 : the retention time of the latter peak in the adjacent two peaks; t R1 : the retention time ...

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Abstract

The invention discloses a method for measuring honeysuckle active ingredient by adopting HPLC (High Performance Liquid Chromatography). The method comprises the following steps: performing specificitytest, linear relation examination, accuracy test, repeatability test, stability test and sampling recovery test respectively under the chromatographic condition; the chromatographic condition is thata detector adopts a diode array detector, a chromatographic column is a chromatographic column (150mm*4.6mm, 5um) of UMC-Pack ODS-A C18, the moving phase is acetonitrile (A) to 0.6% of acetic acid (B), wherein the volume fraction of A is 8% to 28%, the washing time is 0 to 30 min, and the volume fraction of B is 92% to 72%, the detection wavelength is 330 nm, the flow rate is 1.0 mL / min, and thecolumn temperature is 25 degrees centigrade; and the proportional relation of active ingredients in sample solution and those in reference substance solution is the same. According to the method provided by the invention, HPLC (High Performance Liquid Chromatography) and UV detection are used together, eight effective constituents and the content thereof of honeysuckle 50-percent methanol extractcan be detected; methodological investigation shows that the method has the advantages of goodness, stability, accuracy, sensitivity, high efficiency and high recovery.

Description

technical field [0001] The invention belongs to the technical field of detection of traditional Chinese medicine components. More specifically, it relates to a method for determining the active components of honeysuckle by HPLC. Background technique [0002] Honeysuckle is recorded by (1963 edition) Liang · "Famous Doctors", it is the dry flower bud of Lonicerae plant, which is drought-resistant and cold-resistant. (1977 Edition) "Chinese Pharmacopoeia", Lonicera japonica Thunb., Lonicera confusa DC., etc. were selected as the original plants of honeysuckle. (2005 edition) "Chinese Pharmacopoeia" divides honeysuckle into honeysuckle and mountain silver, and includes a variety of related honeysuckle plants. It is determined that the authentic product of medicinal honeysuckle is Lonicera japonica Thunb. Lonicera japonica Thunb. has a high yield in China, is widely distributed, and is cheap and easy to obtain, which provides a source guarantee for subsequent research. [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8809
Inventor 王建舫贺敦严朴美憬崔德凤
Owner BEIJING UNIV OF AGRI
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