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A[beta]1-42 detection reagent strip and application of same

A technology for detection reagents and reagent strips, applied in the field of protein detection, can solve the problems of inapplicability for early diagnosis of Alzheimer's disease, low signal-to-noise ratio, poor sensitivity, etc., to prevent cross-contamination, avoid contamination, and increase accuracy. Effect

Inactive Publication Date: 2018-11-16
苏州仁端生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, enzyme-linked immunoassay cannot avoid the interference of the background signal of the external excitation light source, the signal-to-noise ratio is low, and the sensitivity is poor, so it is not suitable for the early diagnosis of Alzheimer's disease

Method used

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  • A[beta]1-42 detection reagent strip and application of same
  • A[beta]1-42 detection reagent strip and application of same
  • A[beta]1-42 detection reagent strip and application of same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] The assembly of embodiment 1 reagent strip

[0057] The southeast isometric schematic diagram of the reagent strip of this embodiment is as follows: figure 1 As shown, the right view schematic diagram is shown as figure 2 As shown, the schematic diagram of partial southeast isometric section is as follows image 3 As shown, the schematic diagram of the overall southeast isometric section is as follows Figure 4 shown.

[0058] The reagent strip 1 includes a liquid storage chamber layer, a flow channel layer and a reaction chamber layer arranged sequentially from top to bottom;

[0059] The liquid storage chamber layer is provided with seven liquid storage chambers 4, which are respectively a magnetic particle liquid storage chamber 10, a first cleaning liquid storage chamber 11, a second cleaning liquid storage chamber 12, a detection antibody liquid storage chamber 13, and a second cleaning liquid storage chamber. Three cleaning liquid storage chambers 14, fourth ...

Embodiment 2

[0077] Example 2 Aβ 1-42 Testing of Standards

[0078] The reagent strip of this embodiment is formed by injection molding of polymethyl methacrylate (PMMA), and the liquid storage bag is prepared by using aluminum-plastic composite material.

[0079] 50 μL of anti-Aβ 1-42 Magnetic particle solution, 100 μL first washing solution, 100 μL second washing solution, 100 μL alkaline phosphatase-labeled anti-Aβ 1-42 After the antibody, 100 μL of the third cleaning solution, 100 μL of the fourth cleaning solution and 100 μL of the alkaline phosphatase substrate AMPPD reaction solution are injected into the storage bag, it is sealed by welding technology and placed in the magnetic particle storage chamber 10 and the first cleaning solution respectively. In the liquid storage chamber 11, the second cleaning liquid storage chamber 12, the detection antibody liquid storage chamber 13, the third cleaning liquid storage chamber 14, the fourth cleaning liquid storage chamber 15 and the ch...

Embodiment 3

[0091] Example 3 Aβ 1-42 Testing of Clinical Samples

[0092] The reagent strip of this embodiment is formed by injection molding of acrylonitrile-butadiene-styrene copolymer (ABS), and the liquid storage bag is made of aluminum-plastic composite material.

[0093] 50 μL of anti-Aβ 1-42 Magnetic particle solution, 100 μL first washing solution, 100 μL second washing solution, 100 μL alkaline phosphatase-labeled anti-Aβ 1-42 After the antibody, 100 μL of the third cleaning solution, 100 μL of the fourth cleaning solution and 100 μL of the alkaline phosphatase substrate AMPPD reaction solution are injected into the storage bag, it is sealed by welding technology and placed in the magnetic particle storage chamber 10 and the first cleaning solution respectively. In the liquid storage chamber 11, the second cleaning liquid storage chamber 12, the detection antibody liquid storage chamber 13, the third cleaning liquid storage chamber 14, the fourth cleaning liquid storage chamber...

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Abstract

The invention provides an A[beta]1-42 detection reagent strip and an application of same. The reagent strip (1) includes, successively from top to bottom, a liquid storage chamber layer, a flow channel layer, and a reaction chamber layer. At least one liquid storage chamber (4) is disposed in the liquid storage chamber layer. At least one reaction chamber (2) is disposed in the reaction chamber layer. A microfluidic channel (8) is disposed in the flow channel layer and communicates the liquid storage chamber (4) with the reaction chamber (2). The liquid storage chamber (4) is filled with a detection reagent which includes any one of, or a combination of at least two of, A[beta]1-42 capture antibody coupling magnetic particles, a cleaning liquid, an A[beta]1-42 detection antibody, or a reaction solution. On the basis of microfluidic technology, the detection reagents are integrated in the reagent strip, so that reagent pollution is avoided and transportation cost is reduced. The reagentstrip can be loaded on a detection device to perform automatic molecular detection. The reagent strip can eliminate experimental error caused by pollution existing in an opened system and manual operation, and has important application value in early-stage detection of the A[beta]1-42.

Description

technical field [0001] The invention belongs to the field of protein detection and relates to an Aβ 1-42 Detection reagent strip and its application, especially related to a kind of Aβ based on magnetic particles and chemiluminescence technology 1-42 Detection reagent strips and their applications. Background technique [0002] According to statistics, there are nearly 30 million Alzheimer's disease (Alzheimer disease, AD) patients in the world. The harm of Alzheimer's disease to the elderly is extremely serious. The average survival period after diagnosis is only 10 years. It is still a malignant senile mental disease that cannot be cured in the medical field. For people over the age of 60, the incidence of Alzheimer's disease increases dramatically with age. In China, the incidence rate of Alzheimer's disease among people over 65 years old is 7%, that of people over 85 years old is 25%, and that of people over 95 years old is as high as 60%. Early Alzheimer's disease h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N21/76
CPCG01N21/76G01N33/68
Inventor 周小进缪志刚
Owner 苏州仁端生物医药科技有限公司
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