Preparation method of microneedle array biosensor

Abstract

The invention discloses a preparation method of a microneedle array biosensor. The preparation method of the microneedle array biosensor comprises the following steps: making a conductive microneedlearray with rough surface; modifying a biological recognition molecule on the rough surface of the microneedle array; adding a degradable macromolecular material on the surface, on which the biologicalrecognition molecule is modified, of the microneedle array. The microneedle array in the microneedle array biosensor has the rough surface, so that protection of the biological recognition molecule modified on the surface of the microneedle array in the puncture process is benefited; furthermore, the degradable micromolecular material added on the outer layer of the microneedle array can realizesecondary protection of the biological recognition molecule.

Description

technical field [0001] The invention relates to the field of biomedical engineering, in particular to a method for preparing a microneedle array biosensor. Background technique [0002] The microneedle array biosensor is a biorecognition molecule modified on the surface of the microneedle array. In the in vivo detection, after the microneedle array biosensor punctures into the skin, the biorecognition molecule directly contacts with the body fluid, and the biorecognition molecule will specifically interact with the tissue fluid. The biomolecules are specifically combined, and electrons are generated at the same time, and the concentration of biomolecules is expressed in the form of electrical signals. [0003] The use of microneedle array biosensors for in vivo detection has the advantages of little damage to human skin, no pain or slight pain, skin can be restored to its original state in a short time, and microneedle arrays inserted into the human dermis will not cause bio...

AI Technical Summary

Problems solved by technology

For example, for blood glucose detection, which is common in in vivo detection, the use of microneedle array biosensors for blood glucose detection does not require blood collection, and can directly measure the glucose content in interstitial fluid. The glucose content in interstitial fluid is basically the same as the blood glucose content in blood. There is a delay of 5-10 minutes, and if the common blood glucose meter on the market is used for blood sugar testing, patients need to measure blood sugar anytime and anywhere by fingertip blood, but fingertip blood will cause pain, and fingertip blood Bloody wounds cannot heal within minutes and are prone to infection

[0004] Although microneedle array biosensors can be used for in vivo detection, the materials traditionally used to make microneedle arrays are mainly metals, single crystal silicon and polymers, and single crystal silicon microneedle arrays are relatively brittle, causing the microneedles to easily break

Polymer microneedle arrays are not strong enough to pierce the stratum corneum. Metal microneedle arrays are relatively mature and safe, but these types of microneedle arrays all have a common shortcoming, that is, the prepared microneedle arrays The arrays are all smooth surfaces, and the biorecognition molecules modified on the surface of the microneedle array will fall off due to friction during the puncture process, causing the microneedle array entering the body to fail to achieve the purpose of in vivo detection

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