Biologically active peptide kss1b and its preparation method and application

A kss1b, biologically active peptide technology, applied in the preparation methods of peptides, chemical instruments and methods, peptides, etc., can solve the problems of impossible activity, high technical threshold, low degree of product homogeneity, etc., and achieve obvious selectivity. Effect
CN108840910BActive Publication Date: 2021-12-14INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
Publication Date
2021-12-14

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Abstract

The invention belongs to the technical field of polypeptide preparation, and specifically discloses a bioactive peptide kss1b, a preparation method and application thereof. The present invention provides an ideal kss1b recombinant expression scheme using Escherichia coli by selecting a suitable expression strain and a suitable carrier and adopting an automatic induction method. This method can stably obtain 1 mg / L highly pure recombinant kss1b (rkss1b), the amino acid sequence of which is shown in SEQ ID NO.11. rkss1b can significantly inhibit TTX-S-type voltage-sensitive sodium channel (VGSC) current on rat DRG cells at 1 μM level.
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Description

technical field

[0001] The invention belongs to the technical field of polypeptide preparation, in particular, it relates to bioactive peptide kss1b and its preparation method and application. Background technique

[0002] The traditional Chinese medicine centipede is to use the whole body of the giant centipede with few spines of the Centipede family, and the main active component of it is centipede toxin. The centipede toxin has high medicinal value, and has the functions of attacking poison and dispelling stagnation, quenching wind and relieving pain, and anti-cancer. Its medicinal value is highly valued by domestic and foreign medical experts. Centipede toxin contains quite a lot of small peptide molecules, which have different physiological activities and are potential medicinal molecules. However, compared with scorpion toxin, there are fewer researches on the activity of single active peptide molecules derived from centipede in my country.

[0003] kss1b is a toxin o...

Claims

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