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Rapid quantitative method for microcystin MC-LR in growth cycle of microcystis aeruginosa

A technology of MC-LR, Microcystis aeruginosa, applied in measurement devices, color/spectral characteristic measurement, instruments, etc., can solve the problems of high sample loss, low efficiency and high cost, and achieve the effect of simple operation

Inactive Publication Date: 2018-11-20
SHANGHAI APPLIED TECHNOLOGIES COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to solve the above-mentioned deficiencies and provide a kind of microcystin MC-LR rapid quantitative method in the growth cycle of Microcystis aeruginosa. Solve technical problems such as low efficiency, high cost, high sample loss and operator safety in the traditional quantitative method of Microcystin aeruginosa MC-LR

Method used

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  • Rapid quantitative method for microcystin MC-LR in growth cycle of microcystis aeruginosa
  • Rapid quantitative method for microcystin MC-LR in growth cycle of microcystis aeruginosa
  • Rapid quantitative method for microcystin MC-LR in growth cycle of microcystis aeruginosa

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Embodiment 1

[0026] A rapid quantitative method for microcystin MC-LR in the growth cycle of Microcystis aeruginosa, the specific steps are as follows:

[0027] (1) Determination of Microcystin MC-LR by High Performance Liquid Chromatography

[0028] Take algae liquid samples newly inoculated with Microcystis aeruginosa for 0, 20, 40, 60, 80, and 90 hours, and after the algae liquid samples are processed, use high-performance liquid chromatography to measure the concentration of algal toxin MC-LR in the samples.

[0029] The conditions of the method are that the volume ratio of mobile phase methanol to water is 65:35, the flow rate is 1.0mL / min, the column temperature is 40°C, the ultraviolet detection wavelength is 238nm, the injection volume is 10μL, and the retention time is about 4min.

[0030] (2) Determination of UV absorbance of Microcystis aeruginosa liquid sample

[0031] Take the algae liquid samples newly inoculated with Microcystis aeruginosa for 0, 20, 40, 60, 80, and 90 hour...

Embodiment 2

[0038] A rapid quantitative method for microcystin MC-LR in the growth cycle of Microcystis aeruginosa, the specific steps are as follows:

[0039] (1) Cultivate the sample Microcystis aeruginosa used for toxicological experiments for 1-2 weeks, sterilize and then inoculate, the culture medium is BG-11, inoculate into a sterilized Erlenmeyer flask, the inoculation ratio is about 1:5 Or 1:6, within 96 hours after inoculation, it is desired to quickly and quantitatively measure the algal toxin content in the sample.

[0040] (2) Determination of the number of algae cells in Microcystis aeruginosa algae liquid sample

[0041] Take the algae liquid samples at two different time points within 96 hours in step (1), move a little of the algal liquid samples into the counting chamber of the hemocytometer, observe and count under 40 times of optical microscope, Figure 5 , Image 6 The number of algal cells in the algae liquid samples in the counting chamber of the hemocytometer unde...

Embodiment 3

[0044] A rapid quantitative method for microcystin MC-LR in the growth cycle of Microcystis aeruginosa, the specific steps are as follows:

[0045] The sample Microcystis aeruginosa used for toxicological experiments was cultivated for 1-2 weeks, sterilized and then inoculated, the culture solution was BG-11, and inoculated to a sterilized Erlenmeyer flask, the inoculation ratio was about 1:5 or 1: 6. Within 96 hours after inoculation, it is necessary to quickly and quantitatively measure the algal toxin content in the sample.

[0046] (2) Determination of UV absorbance of Microcystis aeruginosa liquid sample

[0047] Take algae liquid samples at two different time points within 96 hours in step (1), move the algae liquid samples into a cuvette, use a UV spectrophotometer, adjust to zero with double distilled water, and measure the corresponding time points at 680nm. The absorbance of the sample is 0.175A, 0.364A.

[0048] Utilize the proportional relationship in Example 1, th...

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Abstract

The invention relates to a rapid quantitative method for microcystin MC-LR in a growth cycle of microcystis aeruginosa. The concentration of the MC-LR within 96 hours in the growth cycle of inoculatedmicrocystis aeruginosa is determined by a high performance liquid chromatography; at the same time, the number of algae cells is calculated by utilizing a microsphere counting chamber under a microscope; absorbance determination of 680 nm is performed on the microcystis aeruginosa by utilizing an ultraviolet spectrophotometer; direct proportional linear relationships of an algal toxin, absorbanceand the number of the algae cells are separately established; and in subsequent algal toxin toxicology experiments, the concentration of the algal toxin MC-LR of a same period can be quantitatively determined by determining the absorbance or the number of the algae cells of the microcystis aeruginosa within 96 hours in the growth cycle. Compared with the prior art, the rapid quantitative method has the advantages of speediness, low cost, small sample loss and nontoxicity, and can solve the technical problems of low efficiency, high cost, huge sample loss, operator safety, and the like in a traditional quantitative method of the microcystin MC-LR.

Description

[technical field] [0001] The invention belongs to the technical field of environmental protection, in particular to a rapid quantitative method for microcystin MC-LR in the growth cycle of Microcystis aeruginosa. [Background technique] [0002] The mass reproduction of cyanobacteria leads to the lack of oxygen in the water body and the deterioration of water quality. The cyanobacteria bloom causes a sharp decrease in the biodiversity of the water body. Among them, Microcystis aeruginosa is one of the algae that form the bloom, and it can produce microcystin, which seriously threatens to other aquatic organisms and even human health. At the same time, various pollutants in the environment have complex effects on the growth of cyanobacteria. In order to effectively control cyanobacteria blooms, the monitoring of Microcystis aeruginosa is particularly important. [0003] At present, the detection methods of algal algae toxins can be divided into chemical analysis detection met...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/74G01N21/31G01N15/10
CPCG01N15/10G01N21/31G01N30/02G01N30/74G01N2015/1006G01N2015/1022
Inventor 王春晖叶璟陈佳文黄晨
Owner SHANGHAI APPLIED TECHNOLOGIES COLLEGE
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