Supercharge Your Innovation With Domain-Expert AI Agents!

Method for protecting catalyzing activity of nanometer platinum particles by glycerin

A catalytic activity and nano-platinum technology, which is applied in the field of nano-platinum particles, can solve the problems of reduced catalytic activity of nano-platinum and limitation of nano-platinum, and achieve good biocompatibility, good biocompatibility, and stable catalytic activity.

Active Publication Date: 2018-11-23
XIAMEN UNIV
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the problem of reduced catalytic activity of nano-platinum limits the application of nano-platinum and needs to be solved urgently.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for protecting catalyzing activity of nanometer platinum particles by glycerin
  • Method for protecting catalyzing activity of nanometer platinum particles by glycerin
  • Method for protecting catalyzing activity of nanometer platinum particles by glycerin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Synthesis of nano-platinum particles and stability study of the catalytic activity of nano-platinum particles resuspended in different solvents

[0028] Take a round bottom flask, soak it in aqua regia overnight and wash it with deionized water. Draw 45 mL of deionized water into the round bottom flask, and add 0.5 mL of 100 mmol / L chloroplatinic acid aqueous solution, stir and heat. After boiling, quickly add 5 mL of 0.4 mol / L ascorbic acid aqueous solution, and continue stirring. After the solution turns black, continue to heat and stir for 30 minutes to promote the stability of the platinum nanoparticles. Then, it was cooled to room temperature under stirring to obtain a nano-platinum particle solution, and the platinum element concentration in the nano-platinum particle solution was 1 mmol / L. Take 1 μL of nano-platinum particle solution, drop it on the supporting copper grid, and take the transmission electron microscope picture, the results are as foll...

Embodiment 2

[0030] Example 2: Protective effect of glycerol on the catalytic activity of surface-modified biotin nano-platinum particles

[0031] In order to further apply glycerol-protected platinum nanoparticles to the fields of biodetection and bioanalysis, it is necessary to modify functional molecules, such as biotin, on the surface of platinum nanoparticles. The steps to modify biotin are as follows:

[0032] Nano-platinum particles were newly synthesized according to the method in Example 1. Add 60 μL of 1% Tween 20 aqueous solution and 30 μL of 100 μmol / L mercapto-methoxypolyethylene glycol (mPEG-SH) to 6 mL of the newly synthesized nano-platinum particle solution, mix well and then add 120 μmol / L L of biotinylated mercaptopolyethylene glycol (biotin-PEG-SH) 60μL and 0.2mol / L phosphoric acid aqueous solution (H 3 PO 4 ) 300 μL, mix well and incubate at 37°C for 1 h. After incubation, centrifuge at 14,000 rpm for 5 min, remove the supernatant, and precipitate biotin-modified nano...

Embodiment 3

[0034] Embodiment 3: MTS method measures the cytotoxicity of the nano-platinum of glycerol protection

[0035] Taking human colorectal cancer cell SW480 as the model cell, MTS[3-(4,5-diethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-etrazolium,inner salt ] method to measure the viability of SW480 cells incubated with platinum nanoparticles protected by different solvents to test the cytotoxicity of platinum nanoparticles protected by glycerol.

[0036] Nano-platinum particles were newly synthesized according to the method in Example 1. Take 2 parts of the newly synthesized platinum nanoparticle solution, 50mL each, and centrifuge at 14000rpm for 5min to remove the supernatant. Glycerin, spare.

[0037] SW480 cells were digested and dispersed in DMEM medium (containing 10% fetal bovine serum, 1% double antibody), mixed gently and added to 96-well plates, 95 μL / well, and three groups of experiments were set up, respectively: control group, PtNPs- In the PBS...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for protecting catalyzing activity of nanometer platinum particles by glycerin. The method is characterized in that the nanometer platinum particles are dispersed intoa 0.1 to 100% glycerin aqueous solution, or a solvent of a solution containing the nanometer platinum particles is replaced by the 0.1 to 100% glycerin aqueous solution, so that the activity of the nanometer platinum catalyzing the decomposing of hydrogen peroxide can be effectively protected, and the decreasing and even loss of the activity in the storage process is avoided. The nanometer platinum particle can be used for modifying the functional molecules at the surface, can reach good biocompatibility, and can be applied to the fields of biological analysis or biomedicines.

Description

technical field [0001] The invention belongs to the technical field of preparing nano-platinum particles, in particular to a method for protecting the catalytic activity of nano-platinum particles by using glycerol, which can obtain highly stable and good biocompatibility nano-platinum particles. Background technique [0002] Nanoparticles have a high specific surface area and diverse shapes, so they have strong catalytic activities such as catalyzing the decomposition of hydrogen peroxide to generate oxygen, and have become one of the research hotspots in recent years. In particular, nano-platinum particles with a variety of functional molecules on the surface are widely used in electrocatalysis, bioanalysis, chemical / biological sensing, fuel cells and other fields. However, the stability of catalytic activity of nano-platinum is poor. Due to the agglomeration of nanoparticles and other reasons, the phenomenon of irregular reduction in catalytic activity often occurs durin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): B01J33/00B01J35/02B01J31/28B01J23/42
CPCB01J23/42B01J31/28B01J33/00B01J35/40
Inventor 杨朝勇陈小锋杜雅慧朱志
Owner XIAMEN UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com