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A method of using glycerin to protect the catalytic activity of nano-platinum particles

A catalytic activity, nano-platinum technology, applied in the field of nano-platinum particles, can solve the problems of reducing the catalytic activity of nano-platinum, restricting nano-platinum, etc., and achieve the effect of good biocompatibility, good biocompatibility and wide application range

Active Publication Date: 2020-11-20
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the problem of reduced catalytic activity of nano-platinum limits the application of nano-platinum and needs to be solved urgently.

Method used

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  • A method of using glycerin to protect the catalytic activity of nano-platinum particles
  • A method of using glycerin to protect the catalytic activity of nano-platinum particles
  • A method of using glycerin to protect the catalytic activity of nano-platinum particles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Synthesis of nano-platinum particles and stability study of the catalytic activity of nano-platinum particles resuspended in different solvents

[0028] Take a round bottom flask, soak it in aqua regia overnight and wash it with deionized water. Draw 45 mL of deionized water into the round bottom flask, and add 0.5 mL of 100 mmol / L chloroplatinic acid aqueous solution, stir and heat. After boiling, quickly add 5 mL of 0.4 mol / L ascorbic acid aqueous solution, and continue stirring. After the solution turns black, continue to heat and stir for 30 minutes to promote the stability of the platinum nanoparticles. Then, it was cooled to room temperature under stirring to obtain a nano-platinum particle solution, and the platinum element concentration in the nano-platinum particle solution was 1 mmol / L. Take 1 μL of nano-platinum particle solution, drop it on the supporting copper grid, and take the transmission electron microscope picture, the results are as foll...

Embodiment 2

[0030] Example 2: Protective effect of glycerol on the catalytic activity of surface-modified biotin nano-platinum particles

[0031] In order to further apply glycerol-protected platinum nanoparticles to the fields of biodetection and bioanalysis, it is necessary to modify functional molecules, such as biotin, on the surface of platinum nanoparticles. The steps to modify biotin are as follows:

[0032] Nano-platinum particles were newly synthesized according to the method in Example 1. Add 60 μL of 1% Tween 20 aqueous solution and 30 μL of 100 μmol / L mercapto-methoxypolyethylene glycol (mPEG-SH) to 6 mL of the newly synthesized nano-platinum particle solution, mix well and then add 120 μmol / L L of biotinylated mercaptopolyethylene glycol (biotin-PEG-SH) 60μL and 0.2mol / L phosphoric acid aqueous solution (H 3 PO 4 ) 300 μL, mix well and incubate at 37°C for 1 h. After incubation, centrifuge at 14,000 rpm for 5 min, remove the supernatant, and precipitate biotin-modified nano...

Embodiment 3

[0034] Embodiment 3: MTS method measures the cytotoxicity of the nano-platinum of glycerol protection

[0035] Taking human colorectal cancer cell SW480 as the model cell, MTS[3-(4,5-diethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-etrazolium,inner salt ] method to measure the viability of SW480 cells incubated with platinum nanoparticles protected by different solvents to test the cytotoxicity of platinum nanoparticles protected by glycerol.

[0036] Nano-platinum particles were newly synthesized according to the method in Example 1. Take 2 parts of the newly synthesized platinum nanoparticle solution, 50mL each, and centrifuge at 14000rpm for 5min to remove the supernatant. Glycerin, spare.

[0037] SW480 cells were digested and dispersed in DMEM medium (containing 10% fetal bovine serum, 1% double antibody), mixed gently and added to 96-well plates, 95 μL / well, and three groups of experiments were set up, respectively: control group, PtNPs- In the PBS...

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Abstract

The invention discloses a method of using glycerin to protect the catalytic activity of platinum nanoparticles. The platinum nanoparticles are dispersed in a 0.1-100% glycerin aqueous solution, or the solvent of the solution containing platinum nanoparticles is replaced with 0.1-100% glycerol. The aqueous solution can effectively protect the activity of nanoplatinum in catalyzing hydrogen peroxide decomposition and prevent the activity from being reduced or even lost during storage. The platinum nanoparticles obtained by the present invention can modify functional molecules on the surface and have good biocompatibility, so they can be used in fields such as bioanalysis or biomedicine.

Description

technical field [0001] The invention belongs to the technical field of preparing nano-platinum particles, in particular to a method for protecting the catalytic activity of nano-platinum particles by using glycerol, which can obtain highly stable and good biocompatibility nano-platinum particles. Background technique [0002] Nanoparticles have a high specific surface area and diverse shapes, so they have strong catalytic activities such as catalyzing the decomposition of hydrogen peroxide to generate oxygen, and have become one of the research hotspots in recent years. In particular, nano-platinum particles with a variety of functional molecules on the surface are widely used in electrocatalysis, bioanalysis, chemical / biological sensing, fuel cells and other fields. However, the stability of catalytic activity of nano-platinum is poor. Due to the agglomeration of nanoparticles and other reasons, the phenomenon of irregular reduction in catalytic activity often occurs durin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J33/00B01J35/02B01J31/28B01J23/42B01J35/00
CPCB01J23/42B01J31/28B01J33/00B01J35/40
Inventor 杨朝勇陈小锋杜雅慧朱志
Owner XIAMEN UNIV
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