A kind of mercury ion detection reagent and preparation method and application thereof

A technology for detecting reagents and mercury ions, which is applied in the field of environmental chemistry, can solve the problems that the detection method cannot meet the actual needs, the requirements for the ability of the experimenters are high, and the cost of electrode production is high, so as to achieve industrial application, good selectivity, and convenient operation. Effect

Active Publication Date: 2021-03-23
HUNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The microbial detection method has great advantages in the simultaneous analysis of a large number of samples. Its advantages are simple operation, economical and practical, and certain sensitivity. However, this method can only detect biologically active substances, and the analysis takes a long time and has poor specificity.
Electrochemical determination methods also have limitations such as high electrode production costs, and the detection procedures of immunoassay, inductively coupled plasma (IC-MS), atomic absorption spectrometry (AAS), and atomic fluorescence spectrometry (AFS) are more complicated and difficult for experimenters. The ability requirements are high, and it is not easy to detect Hg 2+ The existence of these problems makes the existing detection methods still unable to meet the actual needs
In addition, the existing detection methods still have problems such as poor detection sensitivity, difficult operation, high detection cost, harsh detection conditions and difficult to control, and easy to cause environmental pollution.

Method used

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  • A kind of mercury ion detection reagent and preparation method and application thereof
  • A kind of mercury ion detection reagent and preparation method and application thereof
  • A kind of mercury ion detection reagent and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0046] A mercury ion detection reagent, the detection reagent comprises nano gold particle solution and dithiothreitol solution.

[0047] In this embodiment, the initial concentration of the gold nanoparticle solution is 2.2nM; the initial concentration of the dithiothreitol solution is 20mM.

[0048] In this embodiment, the volume ratio of the dithiothreitol solution and the gold nanoparticle solution is 1:200.

[0049] In this embodiment, the particle diameter of the gold nanoparticles in the gold nanoparticles solution is 13nm.

[0050] In this embodiment, the pH value of the detection reagent is 6.6.

[0051] A preparation method of the mercury ion detection reagent of the above-mentioned embodiment of the present invention, comprising the following steps:

[0052] (1) Preparation of nano-gold particle solution: take 91mL of chloroauric acid solution (solvent is water) with a concentration of 0.1g / L, heat it to boiling on an electric furnace and keep it in a boiling stat...

Embodiment 2

[0060] A preparation method for mercury ion detection reagent, comprising the following steps:

[0061] (1) Preparation of gold nanoparticles solution: same as in Example 1.

[0062] (2) Preparation of detection reagents:

[0063] Take 32 parts of the gold nanoparticle solution in step (1), each 2 mL, and divide them into two groups.

[0064] The first group: adding a volume of 10 μL and an initial molar concentration of DETL solution of 20 mM to each part of gold nanoparticle solution, standing for 2 hours under dark conditions, centrifuging and redissolving three times at a speed of 12000 r / min (centrifugal redissolving With a concentration of 10mM, pH is respectively 4.0, 4.3, 4.6, 5.0, 5.3, 5.6, 6.0, 6.3, 6.6, 7.0, 7.3, 7.6, 8.0, 8.3, 8.6, 9.0 phosphate buffer), each 15min, Detection reagents with pHs of 4.0, 4.3, 4.6, 5.0, 5.3, 5.6, 6.0, 6.3, 6.6, 7.0, 7.3, 7.6, 8.0, 8.3, 8.6, and 9.0 were obtained.

[0065] The second group: adding 10 μL of deionized water to each part ...

Embodiment 3

[0071] The application of a mercury ion detection reagent in detecting mercury ions comprises the following steps:

[0072] Take 10 parts of the mercury ion detection reagent prepared in Example 1, and add 30 μL of mercury ion solution with a concentration of 10 μM to each detection reagent to form a reaction system and start the reaction. The temperature during the reaction is 15 ° C, 18 °C, 20 °C, 24 °C, 28 °C, 30 °C, 32 °C, 34 °C, 38 °C and 40 °C, and the pH value of the system during the reaction was 6.6. After reacting for 20 minutes, detect the ultraviolet-visible absorption spectra of each group of systems, and compare the absorption values ​​at 650nm and 525nm to obtain a ratio (A 650 / A 525 ), see the results Figure 4 .

[0073] Figure 4 It is a histogram of the ratio of absorbance at 650 nm to 525 nm corresponding to different reaction temperatures in Example 3 of the present invention. Such as Figure 4 As shown, with the increase of temperature, A 650 / A ...

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Abstract

The invention discloses a mercury ion detection reagent as well as a preparation method and application thereof. The detection reagent is prepared from a gold nanoparticle solution and a dithiothreitol solution. The preparation method comprises the steps of adding the dithiothreitol solution into the gold nanoparticle solution, oscillating under the condition of keeping out of the sun, centrifuging and re-dissolving to obtain the detection reagent. The detection reagent provided by the invention has the advantages of being high in sensitivity, good in selectivity, strong in specificity and harmless to the environment, and the like; the detection reagent has a wide mercury ion detection range and is low in lower limit of detection, wherein the detection limit is 0.024mu M; the detection reagent can meet the standard limit value of the national standard; the preparation method of the detection reagent is simple in process and convenient to operate. The detection reagent provided by the invention can be used for qualitative and quantitative detection of the mercury ions, does not require special expensive instruments, has the advantages of being simple in process, convenient to operate, simple and rapid, mild in detection conditions, easy to control, low in cost, and the like, and has the advantages of high detection sensitivity, good detection selectivity, strong detection specificity and the like for the mercury ions, thus having very good use value and application prospect.

Description

technical field [0001] The invention belongs to the technical field of environmental chemistry, and relates to a mercury ion detection reagent, a preparation method and an application thereof. Background technique [0002] Mercury in blood and tissues can combine with sulfhydryl groups in proteins and enzyme systems, inhibit their function, and even inactivate them, such as combining with sulfhydryl-containing lipoic acid, pantetheine and coenzyme A, etc., affecting the brain Metabolism of pyruvate. Mercury acts on reduced glutathione, impairing its redox function. There are many non-functional sulfhydryl groups in the body, which constitute the hydrophobic part of the globulin. If combined with mercury, the volume will shrink, the entire molecule will be distorted and deformed, and the enzyme will lose its activity. High concentrations of mercury can also combine with amino groups, hydroxyl groups, phosphoryl groups, carboxyl groups, etc. in enzymes, causing corresponding...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31G01N21/33G01N21/78
CPCG01N21/31G01N21/33G01N21/78
Inventor 刘希贵黄丹莲赖萃许飘曾光明秦蕾易欢柳诗语张玉锦
Owner HUNAN UNIV
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