A method for biologically treating boron-containing wastewater and increasing the oil production of microalgae
A technology for biological treatment and microalgae oil, applied in biological water/sewage treatment, chemical instruments and methods, water/sludge/sewage treatment, etc., can solve the problems of short treatment time, unsuitable for large-scale application, and low cost.
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Embodiment 1
[0031] Based on BG11 medium, the concentration of B (in BO 3 3- -B) is 0.5mg / L, add Chlorella regularis var.minima as the experimental species to make the initial biomass 0.1g / L, add 10g / L glucose as carbon source, Perform heterotrophic culture.
[0032] During the cultivation process, the biomass gradually increased, and stabilized after the third day, and reached the maximum biomass after 6 days of cultivation, the maximum biomass was 3.8g / L, the boron removal rate reached 78%, and the boron removal capacity of Chlorella was 0.1mg / g. The oil content is 20%, and the oil output is 0.7g / L.
[0033] The boron removal ability of the chlorella refers to the mass of boron that can be removed by the chlorella per unit mass during the treatment of boron-containing wastewater. Calculated as follows:
[0034] Boron removal capacity (mg / g) = [initial boron concentration (mg / L) - boron concentration after treatment (mg / L)] / [microalgae concentration after treatment (g / L) - initial...
Embodiment 2
[0036] Adjust on the basis of BG11 medium, wherein B concentration (in BO 3 3- -B) is 10mg / L, add Chlorella regularis var.minima (purchased from the freshwater algae species bank of Chinese Academy of Sciences) as the experimental algae species to make its initial biomass 0.1g / L, add 10g / L glucose as carbon Sources were cultured heterotrophically at 25°C.
[0037] During the cultivation process, the biomass gradually increased and stabilized after the third day, and reached the maximum biomass after 6 days of cultivation, the maximum biomass was 3.8g / L, the boron removal rate reached 34%, and the boron removal capacity of Chlorella was 1.0mg / g. The oil content is 23%, and the oil yield is 0.9g / L. The growth of microalgae is basically not affected, compared with Example 1, the biomass has no significant change, the boron removal ability is increased by 9 times, the oil content is increased by 15%, and the oil output is increased by 29%.
Embodiment 3
[0039] Adjust on the basis of BG11 medium, wherein B concentration (in BO 3 3- -B) is 25mg / L, Chlorella regularis is added as the experimental algae species to make the initial biomass 0.1g / L, 10g / L glucose is added as a carbon source, and heterotrophic culture is carried out at 25°C.
[0040] During the cultivation process, the biomass gradually increased, and it tended to be stable after the third day, and reached the maximum biomass after 6 days of cultivation, the maximum biomass was 3.9g / L, the boron removal rate reached 33%, and the boron removal capacity of Chlorella was 2.8mg / g. The oil content is 34%, and the oil yield is 1.3g / L. The growth of microalgae was basically not affected, and compared with Example 1, the biomass increased slightly by 3%; the removal capacity of boron was increased by 27 times, the oil content was increased by 70%, and the oil yield was increased by 86%.
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