Preparation method of sulfur autotrophic denitrification bacterium immobilized particles

A denitrifying bacteria, sulfur autotrophic technology, applied in biochemical equipment and methods, microorganism-based methods, immobilized on/in organic carriers, etc., can solve the problems of low efficiency, easy loss of free bacteria, etc. Wide range of sources, favorable for adhesion, density-increasing effects

Pending Publication Date: 2018-11-30
CHINA UNIV OF GEOSCIENCES (BEIJING)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to solve the problems of low efficiency of sulfide autotrophic denitrification and easy loss of free bacteria in the prior art, and proposes a preparation method of immobilized granules of sulfur autotrophic denitrifying bacteria, aiming at improving the efficiency of sulfur autotrophic denitrification bacteria. Improve the mechanical properties of denitrifying bacteria immobilized particles, reduce the adaptation time of microorganisms, and improve the activity of microorganisms

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  • Preparation method of sulfur autotrophic denitrification bacterium immobilized particles
  • Preparation method of sulfur autotrophic denitrification bacterium immobilized particles
  • Preparation method of sulfur autotrophic denitrification bacterium immobilized particles

Examples

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Embodiment 1

[0030] A kind of preparation method of sulfur autotrophic denitrifying bacteria immobilized granule that the present invention proposes, comprises the following steps:

[0031] S1. Add 10g of polyvinyl alcohol and 0.67g of xanthan gum into 100ml of deionized water, heat and stir at a temperature of 40°C, and obtain a mixed solution after fully dissolving and cooling to room temperature. Add 0.7g of a particle size of 60 The purpose rice husk is placed in 2ml of bacterial solution with a sludge sedimentation ratio of 20%. After hanging the film in a constant temperature incubator for 24 hours, it is added to the mixed solution together with 10g of pyrite with a particle size of 100 mesh and stirred evenly. forming material A;

[0032]S2. Melt 50 g of boric acid and 10 g of calcium chloride into 1000 ml of deionized water, heat to fully dissolve, then cool at room temperature until the boric acid is saturated and precipitated, and use 0.5 mol / L of sodium hydroxide solution to ad...

Embodiment 2

[0035] A kind of preparation method of sulfur autotrophic denitrifying bacteria immobilized granule that the present invention proposes, comprises the following steps:

[0036] S1. Add 10g of polyvinyl alcohol and 0.67g of xanthan gum into 100ml of deionized water, heat and stir at a temperature of 50°C, and obtain a mixed solution after fully dissolving and cooling to room temperature. The purpose rice husk is placed in 1ml of bacterial solution with a sludge sedimentation ratio of 30%. After hanging the film in a constant temperature incubator for 36 hours, it is added to the mixed solution together with 5g of pyrite with a particle size of 140 mesh. After stirring evenly forming material A;

[0037] S2. Melt 25g of boric acid and 5g of calcium chloride into 500ml of deionized water, heat to fully dissolve, then cool at room temperature until the boric acid is saturated and precipitated, use 0.3mol / L sodium hydroxide solution to adjust the pH to 7.2, The supernatant was tak...

Embodiment 3

[0040] A kind of preparation method of sulfur autotrophic denitrifying bacteria immobilized granule that the present invention proposes, comprises the following steps:

[0041] S1. Add 8g of polyvinyl alcohol and 0.67g of xanthan gum into 100ml of deionized water, heat and stir at a temperature of 40°C, and obtain a mixed solution after fully dissolving and cooling to room temperature. Add 0.35g of a particle size of 60 The purpose rice husk is placed in 1ml of bacterial solution with a sludge sedimentation ratio of 20%. After hanging the film in a constant temperature incubator for 12 hours, it is added to the mixed solution together with 5g of pyrite with a particle size of 100 mesh and stirred evenly. forming material A;

[0042] S2. Melt 25g of boric acid and 5g of calcium chloride into 500ml of deionized water, heat to fully dissolve, then cool at room temperature until the boric acid is saturated and precipitated, use 0.3mol / L sodium hydroxide solution to adjust the pH t...

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Abstract

The invention belongs to field of biological treatment of nitrate pollution in groundwater, and particularly relates to a preparation method of sulfur autotrophic denitrification bacterium immobilizedparticles. The preparation method of the sulfur autotrophic denitrification bacterium immobilized particles comprises the following steps that S1, polyvinyl alcohol and xanthan gum are added into deionized water, agricultural waste and sulfide which are subjected to biofilm culturing are added, and thus a material A is formed; S2, boric acid and calcium chloride are molten in the deionized water,a sodium hydroxide solution is added to adjust the pH value, then a supernatant is taken, and thus a solution B is formed; and S3, the material A is taken to be dripped into the solution B to form embedded particles, and after a cross-linking reaction is conducted, the sulfur autotrophic denitrification bacterium immobilized particles are obtained. According to the preparation method of the sulfur autotrophic denitrification bacterium immobilized particles, the mechanical properties and salt stability of the sulfur autotrophic denitrification bacterium immobilized particles can be improved, the denitrification start time is shortened, the denitrification efficiency is improved, and meanwhile, a new idea is provided for secondary utilization of the agricultural waste.

Description

technical field [0001] The invention belongs to the field of biological treatment of groundwater nitrate pollution, in particular to a method for preparing immobilized granules of sulfur autotrophic denitrifying bacteria. Background technique [0002] Nitrate exists widely in nature, and it itself does not cause great harm to the human body, but the nitrite it transforms into in the human body is very toxic. Among them, methemoglobinosis is caused by long-term consumption of food contaminated by nitrite nitrogen. groundwater results. Hemoglobin, which is used to transport oxygen in the blood, is an iron-based compound that can react with nitrite to form methemoglobin, which cannot transport oxygen, thereby affecting the ability to transport oxygen. And nitrite has a greater impact on infants less than three months old than adults, making the content of methemoglobin in infants higher than that of adults. At the same time, nitrite will also react with nitrogen oxide compoun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/08C12N11/10C12R1/01
CPCC12N1/20C12N11/08C12N11/10
Inventor 冯传平薛立静陈男
Owner CHINA UNIV OF GEOSCIENCES (BEIJING)
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