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Fabrication of photoelectrochemical sensors based on multi-level nano-zinc oxide microsphere composites

A nano-zinc oxide, photoelectrochemical technology, applied in the direction of material electrochemical variables, scientific instruments, analytical materials, etc., can solve the problems of cumbersome steps, long detection cycle, low sensitivity, etc., and achieve simple operation, low detection limit, and improved sensitivity Effect

Active Publication Date: 2020-04-28
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the methods for detecting Aβ include solid-state NMR detection method, western blotting method, etc., but these methods have the disadvantages of low sensitivity, long detection cycle, and cumbersome steps. High, good selectivity, fast and easy operation of photoelectrochemical immunoassay method, this method has the advantages of always on-line detection, convenient instrument operation, high specificity, wide detection range, etc., and has broad application prospects in the field of small molecule and protein detection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1 prepares ZnO / Ru(bpy) 3 2+ The specific steps of / Ce-CdS are:

[0044] (1) Dissolve 2 mmol of zinc sulfate heptahydrate, 8 mmol of urea and 0.4 mmol of trisodium citrate in 43 mL of deionized water, stir magnetically for 30 min to form a uniform neutral solution, and place the mixture in 100 mL of polytetrafluoroethylene Reaction in vinyl fluoride autoclave at 120°C for 6 h, the precipitate was centrifugally washed, dried in a vacuum oven for 10 h, and the obtained solid was calcined in a muffle furnace at 300°C for 2 h to finally produce multi-level nano Zinc oxide microspheres are placed in ultrapure water to obtain a ZnO suspension;

[0045] (2) Cut the ITO electrode to a size of 2.5 cm × 0.8 cm, ultrasonically clean it with detergent, acetone, ethanol and ultrapure water for 30 min, and dry it with nitrogen, then modify the ITO electrode with 10 µL of ZnO suspension , and dried at room temperature to obtain an ITO / ZnO electrode;

[0046] (3) The surf...

Embodiment 2

[0047] Example 2 Preparation of ZnO / Ru(bpy) 3 2+ The specific steps of / Ce-CdS are:

[0048] (1) Dissolve 3 mmol of zinc sulfate heptahydrate, 12 mmol of urea and 0.6 mmol of trisodium citrate in 64 mL of deionized water, stir magnetically for 30 min to form a uniform neutral solution, and place the mixture in 100 mL of polytetrafluoroethylene React in a vinyl fluoride autoclave at 120°C for 6 h, wash the precipitate by centrifugation, dry it in a vacuum oven for 12 h, and place the resulting solid in a muffle furnace for calcination at 300°C for 2 h to finally produce a multi-level nano Zinc oxide microspheres are placed in ultrapure water to obtain a ZnO suspension;

[0049] (2) Cut the ITO electrode to a size of 2.5 cm × 0.8 cm, ultrasonically clean it with detergent, acetone, ethanol and ultrapure water for 30 min, and dry it with nitrogen, then modify the ITO electrode with 10 µL of ZnO suspension , and dried at room temperature to obtain an ITO / ZnO electrode;

[0050...

Embodiment 3

[0051] Example 3 Preparation of ZnO / Ru(bpy) 3 2+ The specific steps of / Ce-CdS are:

[0052] (1) Dissolve 3.75 mmol of zinc sulfate heptahydrate, 15 mmol of urea, and 0.75 mmol of trisodium citrate in 80 mL of deionized water, stir magnetically for 40 min to form a uniform neutral solution, and place the mixture in 100 mL of polytetrafluoroethylene React in an ethylene high-pressure reactor at 120°C for 6 h, wash the precipitate by centrifugation, dry it in a vacuum oven for 12 h, and place the resulting solid in a muffle furnace for calcination at 300°C for 2 h to finally produce a multi-level nano-oxidized Zinc microspheres are placed in ultrapure water to obtain a ZnO suspension;

[0053] (2) Cut the ITO electrode to a size of 2.5 cm × 0.8 cm, ultrasonically clean it with detergent, acetone, ethanol, and ultrapure water for 30 min, and dry it with nitrogen, then modify the ITO electrode with 10 µL of ZnO suspension , and dried at room temperature to obtain an ITO / ZnO ele...

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PUM

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Abstract

The invention relates to a preparation method and application of a photoelectric chemical beta-amyloid protein sensor based on multilevel nano-zinc oxide microsphere composite materials, and belongs to the field of photoelectric chemical sensors. Multilevel nano-zinc oxide microspheres are synthesized by a hydrothermal synthesis method and sensitized through Rucbpy and thus the absorption of the multilevel nano-zinc oxide microspheres to visible lights is enhanced; then, cerium-doped cadmium sulfide is subjected to in-situ growth, and the multilevel nano-zinc oxide microsphere composite materials ZnO / Ru(bpy)<3><2+> / Ce-CdS with significantly enhanced and stable photocurrent signals are obtained; the ZnO / Ru(bpy)<3><2+> / Ce-CdS is used as a base material, PS@CuS which has the good insulation property is used as a secondary antibody marker, and thus transfer of light-generated electrons is hindered; through the absorption competition to the visible lights between the PS@CuS and the ZnO / Ru(bpy)<3><2+> / Ce-CdS, the photocurrent signals are also effectively reduced, and through the double-inhibition action, the sensitivity of the sensor is effectively improved, and the detection limit of the sensor is lowered; and then the excellent selectivity of aptamers is combined, thus high sensitivity detection of beta-amyloid protein is achieved, and the method is of great significance to analysis and detection of the beta-amyloid protein.

Description

technical field [0001] The invention belongs to the fields of novel functional nanometer materials, immune analysis and photoelectrochemical sensors, and provides a preparation method and application of a photoelectrochemical β-amyloid sensor based on multilevel nanometer zinc oxide microsphere composite materials. Background technique [0002] Alzheimer's disease (AD) is a common degenerative disease of the central nervous system, which poses severe challenges to the quality of life, health care and social security of the elderly. Senile plaques are a major pathological feature of AD, which are mainly formed by the aggregation of β-amyloid Aβ with neurotoxicity and vascular toxicity. Aβ plays a crucial role in the formation and development of AD. Under normal circumstances, the Aβ produced by cells shows little neurotoxicity, but under abnormal or pathological conditions, Aβ can form neurotoxic fibrous aggregates, triggering abnormal phosphorylation of Tau protein, synapti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30G01N27/327G01N33/531
CPCG01N27/30G01N27/3278G01N33/531
Inventor 范大伟刘昕鲍春竹吴丹张勇马洪敏王欢魏琴杜斌胡丽华
Owner UNIV OF JINAN