Application of traditional Chinese medicine composition in preparation of drugs for treating osteoarthritis

A technology of osteoarthritis and composition, applied in the field of traditional Chinese medicine, can solve problems such as inconclusiveness, and achieve the effect of improving curative effect

Inactive Publication Date: 2018-12-07
JIANGSU KANION PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its pathogenesis is still inconclusive

Method used

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  • Application of traditional Chinese medicine composition in preparation of drugs for treating osteoarthritis
  • Application of traditional Chinese medicine composition in preparation of drugs for treating osteoarthritis
  • Application of traditional Chinese medicine composition in preparation of drugs for treating osteoarthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 The influence of traditional Chinese medicine composition of the present invention on IL-1β-induced chondrocyte inflammation

[0027] 1 Experimental materials and methods

[0028] 1.1 Drugs

[0029] Yaobitong capsule contents.

[0030] 1.2 Materials

[0031] AL104 electronic balance, Mettler-Toledo Instrument Co., Ltd.; centrifuge, Eppendorf 5804R; microplate reader, Flex station3 (MD); carbon dioxide incubator (Thermo); ChemiDoc XRS (Bro-rad).

[0032] Nitric Oxide (NO), Nitric Oxide Synthase (iNOS), Interleukin 1β (IL-1β), Prostaglandin E 2 (PGE 2 ), cyclooxygenase 1 (COX1), matrix metalloproteinase 9 (MMP-9), tumor necrosis factor (TNF-α) and nuclear transcription factor κB (NF-κB) detection kits (Nanjing Jiancheng).

[0033] Animals: SD rats, male, 100-120 g, purchased from Weitong Lihua.

[0034] High glucose DMEM medium, fetal bovine serum was purchased from Gibco; p-P38MAPK antibody was purchased from Abcam, HRP-labeled secondary antibody was p...

Embodiment 2

[0059] Embodiment 2 The influence of Chinese medicine composition of the present invention on the anti-inflammatory action of traumatic osteoarthritis rat model

[0060] 1 Materials and methods

[0061] 1.1 Drugs and reagents

[0062] Yaobitong capsule contents.

[0063] NO and TNF-α detection kits were purchased from Nanjing Jiancheng.

[0064] 1.2 Animals

[0065] Thirty healthy SD rats, half male and half male, weighing 180-220 g, were purchased from Weitong Lihua.

[0066] 1.3 Establishment of traumatic osteoarthritis rat model

[0067] The animals were weighed after routine feeding for 1 week, and the SD rats were divided into normal group, model group, high-dose, medium-dose and low-dose groups of Yaobitong, with 6 rats in each group. In addition to the normal group, the remaining 4 groups injected 10% chloral hydrate (3mg / kg) into the abdominal cavity of SD rats for anesthesia, opened the joint cavity, cut the medial collateral ligament surgically, removed the meni...

Embodiment 3

[0076] Embodiment 3 Chinese medicine composition of the present invention is to the effect of inflammation and anti-inflammation of knee osteoarthritis rats

[0077] 1 Materials and methods

[0078] 1.1 Drugs and reagents

[0079] Yaobitong capsule content.

[0080] COX-2, NO, iNOS detection kits were purchased from Nanjing Jiancheng.

[0081] 1.1 Animals

[0082] Thirty healthy SD rats, half male and half male, weighing 180-220 g, were purchased from Weitong Lihua.

[0083] 1.3 Modeling, grouping and administration of rats

[0084] The animals were weighed after routine feeding for 1 week, and the SD rats were divided into normal group, model group, high-dose, medium-dose and low-dose groups of Yaobitong, with 6 rats in each group.

[0085] Murat method: 4% papain solution and 0.3mol / L cysteine ​​solution were mixed 1:1 for 0.5h to make a mixed solution, and the rest of the rats except the normal group were fixed on the operating table and shaved with a razor blade. Rem...

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Abstract

The invention relates to an application of a traditional Chinese medicine composition in the preparation of drugs for treating osteoarthritis. The traditional Chinese medicine composition comprises following raw materials in parts by weight: 100 to 400 parts of pseudo-ginseng, 200 to 500 parts of ligusticum wallichii, 200 to 500 parts of corydalis tuber, 200 to 500 parts of white peony root, 200 to 500 parts of achyranthes root, 100 to 400 parts of rhizoma cibotii, 100 to 400 parts of cooked rhubarb, and 100 to 400 parts of angelica pubescens. The provided traditional Chinese medicine composition can be used to treat degradation and inflammation of bone joints and cartilage.

Description

technical field [0001] The present invention generally relates to the field of Chinese medicines, in particular to the application of a Chinese medicine composition in the preparation of medicines for treating osteoarthritis. Background technique [0002] Osteoarthritis (OA) is called "Bone Bi" or "Bi Syndrome" in traditional Chinese medicine. It is believed that with the aging of the human body, external evils invade, and the Qi and blood in the liver and kidney veins become imbalanced, leading to the body's reverse disorder, joint disadvantages, and easy Osteoarthritis occurs. Modern doctor trained in Western medicine thinks that osteoarthritis is the slow degenerative change that mainly occurs in bone articular cartilage, and pathology mainly manifests as cartilage softening and erosion. At present, its pathogenesis research is still inconclusive. It is more common in middle-aged and elderly people, and the knee joint and hip joint are most easily affected, which seriou...

Claims

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Application Information

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IPC IPC(8): A61K36/71A61K36/708A61P19/02A61P19/08A61P29/00
CPCA61K36/12A61K36/21A61K36/232A61K36/236A61K36/258A61K36/65A61K36/66A61K36/708A61K36/71A61K2236/331A61K2236/333A61K2236/39A61K2236/51A61P19/02A61P19/08A61P29/00A61K2300/00
Inventor 肖伟金治全刘莉娜周军王振中
Owner JIANGSU KANION PHARMA CO LTD
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