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Method for extracting Prunus cerasifera skin anthocyanin

A technique of plum peel anthocyanin and an extraction method, which is applied to the extraction field of cherry plum peel anthocyanin, can solve problems such as few reports on the extraction process, and achieves that the extract is safe and reliable, inhibits tyrosinase activity well, and is effective. The effect of high content of ingredients

Inactive Publication Date: 2018-12-07
GUANGDONG MARUBI BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are few reports on the extraction process of cherry anthocyanins from cherry plum peel

Method used

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  • Method for extracting Prunus cerasifera skin anthocyanin
  • Method for extracting Prunus cerasifera skin anthocyanin
  • Method for extracting Prunus cerasifera skin anthocyanin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for extracting cherry plum peel anthocyanins, comprising the steps of:

[0032] S1. Cherry plum peel pretreatment: the cherry plum peel is subjected to beating, vacuum freeze-drying, and pulverization pretreatment to obtain cherry plum peel pretreatment powder, wherein the vacuum freeze-drying temperature is -78°C, the time is 48h, the vacuum pressure is 20Pa, and crushed To a particle size of 60 mesh;

[0033] S2. Anthocyanin extraction: Weigh 3.00g cherry plum peel powder, under the conditions of ultrasonic power 600W, solid-liquid ratio 1:30, ethanol concentration 80%, extraction time 60min, extraction temperature 60°C, hydrochloric acid concentration 0.1% Extract to obtain crude cherry plum peel anthocyanin extract;

[0034] The crude cherry plum peel anthocyanin extract was filtered under the conditions of -0.1MPa vacuum and double-layer filter paper with a pore size of 15-20um, and then under the vacuum of -0.1MPa, the rotation speed was 115rpm / min, the ...

Embodiment 2

[0037] A method for extracting cherry plum peel anthocyanins, comprising the steps of:

[0038] S1. Cherry plum peel pretreatment: the cherry plum peel is subjected to beating, vacuum freeze-drying, and crushing pretreatment to obtain cherry plum peel pretreatment powder, wherein the vacuum freeze-drying temperature is -70°C, the time is 40h, the vacuum pressure is 15Pa, and crushed to a particle size of 40 mesh;

[0039] S2. Anthocyanin extraction: Weigh 3.00g cherry plum peel powder, under the conditions of ultrasonic power 500W, solid-liquid ratio 1:20, ethanol concentration 50%, extraction time 100min, extraction temperature 70°C, hydrochloric acid concentration 0.05% extracting, extracting under the conditions of obtaining the crude cherry plum anthocyanin extract, obtaining the crude cherry plum anthocyanin extract;

[0040] The crude cherry plum peel anthocyanin extract was filtered under the conditions of -0.1MPa vacuum and double-layer filter paper with a pore size of ...

Embodiment 3

[0043] A method for extracting cherry plum peel anthocyanins, comprising the steps of:

[0044] S1. Cherry plum skin pretreatment: the cherry plum skin is subjected to beating, vacuum freeze-drying, and pulverization pretreatment to obtain cherry plum skin pretreatment powder, wherein the vacuum freeze-drying temperature is -80°C, the time is 60h, the vacuum pressure is 25Pa, and pulverization to a particle size of 80 mesh;

[0045] S2. Extraction of anthocyanins: Weigh 3.00g of cherry plum peel powder, extract under the conditions of microwave power 400W, solid-liquid ratio 1:30, ethanol concentration 80%, extraction time 60min, and extraction temperature 60°C to obtain cherry plum Picocyanidin crude extract;

[0046] The crude cherry plum peel anthocyanin extract was filtered under the conditions of -0.1MPa vacuum and double-layer filter paper with a pore size of 15-20um, and then under the vacuum of -0.1MPa, the rotation speed was 115rpm / min, the evaporation temperature wa...

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Abstract

The invention discloses a method for extracting Prunus cerasifera skin anthocyanin. The method comprises the following steps: (S1) preprocessing Prunus cerasifera skin: carrying out pulping, vacuum freeze drying and crushing processing on the Prunus cerasifera skin, so as to obtain processed powder of Prunus cerasifera skin; and (S2) extracting anthocyanin: extracting anthocyanin from the processed powder in the step (S1) so as to obtain a Prunus cerasifera skin anthocyanin crude extracting solution, filtering, concentrating to obtain an anthocyanin crude extract, and purifying, so as to obtain the Prunus cerasifera skin anthocyanin. The extraction rate of the anthocyanin extract obtained by virtue of the method is over 3%, the contents of effective components in the anthocyanin extract are high, the impurities are few, the flavonoid content in the Prunus cerasifera skin extract reaches up to 40-70, the anthocyanin extract has relatively good antioxidant effect and inhibition activityto tyrosinase, the total oxidation resistance value is over 1.2mmol, the inhibition activity to tyrosinase reaches over 85%, and the anthocyanin extract is safe and reliable and can be widely appliedto the field of preparation of cosmetics.

Description

technical field [0001] The invention relates to the technical field of anthocyanin extraction, in particular to a method for extracting anthocyanin from cherry plum bark. Background technique [0002] Cherry plum (Prunus cerasifera Ehrh.), also known as sour plum (P.myrobalan loisel) or cherry plum, belongs to the plant of the genus Prunus (Prunus) of the subfamily Prunoideae of the Rosaceae family. Cherry plum has gorgeous flowers, bright fruit and high nutritional value. It can be used for viewing, raw food and processing. Since ancient times, it has been hailed as the "sacred fruit in the snowy region" by ethnic minorities in Xinjiang. The flesh is soft and juicy, and it is a small fruit type. The fruit of most strains is round, and a few are oval, oval, ovate and broad ovate, and the fruit color has 4 types of yellow, red, purple and black. Cherry plum fruit has a complete range of nutrients and is rich in content. It is a rare natural health food, containing 13.6% to 1...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 刘露孙云起孙怀庆裴运林聂艳峰
Owner GUANGDONG MARUBI BIOLOGICAL TECH CO LTD
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