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Detection kit for relapse and drug resistance gene mutation of acute lymphoblastic leukemia (ALL) and application method thereof

An acute lymphocyte and detection kit technology, applied in the fields of medicine and molecular detection, can solve problems such as affecting clinical prognosis and promoting disease recurrence, achieving the effects of strong applicability, reducing false positives, and accurate detection results

Active Publication Date: 2018-12-11
北京海思特医学检验实验室有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Related studies have pointed out that the high expression and mutation of cN-II are related to the drug resistance of cytarabine (Ara-c) and 6-mercaptopurine (6-MP), which promotes disease recurrence and affects the clinical prognosis of ALL.

Method used

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  • Detection kit for relapse and drug resistance gene mutation of acute lymphoblastic leukemia (ALL) and application method thereof
  • Detection kit for relapse and drug resistance gene mutation of acute lymphoblastic leukemia (ALL) and application method thereof
  • Detection kit for relapse and drug resistance gene mutation of acute lymphoblastic leukemia (ALL) and application method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Composition and preparation of embodiment 1 kit

[0046] In the present invention, for acute lymphoblastic leukemia chemotherapy resistance and relapse-related NT5C2 drug resistance gene, a detection kit and application method for acute lymphoblastic leukemia treatment medication and close detection of relapse are developed. This gene is currently the most promising target for the detection of chemotherapy resistance and relapse of acute lymphoblastic leukemia, and can play an important role in the current and future treatment of acute lymphoblastic leukemia.

[0047] The source of the gene sequence detected by the present invention is the NCBI (National Center for Biotechnology Information) gene bank, and the specific identification and functions are shown in Table 1 below:

[0048] Table 1

[0049]

[0050] The domain distribution diagram of the gene-encoded protein detected by the present invention, such as figure 1 shown.

[0051] The invention provides a kit ...

Embodiment 2

[0061] Example 2 Detection and verification report of the kit

[0062] 1. Name of the test procedure (method) of the acute lymphoblastic leukemia drug detection and recurrence monitoring kit of the present invention: NT5C2 gene mutation detection (PCR-generation sequencing method).

[0063] 2. The purpose of verification: through the analysis and evaluation of the accuracy, sensitivity, and precision of the methodology, the method verification of the testing procedure (methodology) is carried out to meet the needs of clinical experiments.

[0064] 3. Experimental materials:

[0065] 3.1. Specimen type: DNA extracted from peripheral blood.

[0066] 3.2. Reagents: This verification experiment uses the conventional reagents of the molecular platform, and all reagents are within the validity period. The main reagent information is shown in Table 4:

[0067] Table 4 Reagent information used for NT5C2-related gene mutation detection verification

[0068]

[0069] 3.3. Instrume...

Embodiment approach

[0082] The specific implementation is as follows:

[0083] In step S1, peripheral blood is used as the sample to be tested. Genomic DNA can be extracted using a conventional kit column extraction method, and operated according to the instructions of the kit used to collect 50 μL of DNA solution with a concentration of 50-200 ng / μL, which can be directly used Before testing or store at -20°C;

[0084] The verification experiment used 7 specimens, and the specimen type was DNA extracted from peripheral blood. See Table 5 for the specimens.

[0085] Table 5. Specimen information used for NT5C2-related gene mutation detection verification

[0086] sample name

sample type

sample number

1

DNA extracted from peripheral blood

p16060306

2

DNA extracted from peripheral blood

p16060608

3

DNA extracted from peripheral blood

p16061215

4

DNA extracted from peripheral blood

p16061242

5

DNA extracted from periphe...

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Abstract

The invention belongs to the field of medicine and molecular detection, in particular to a detection kit for relapse and drug resistance gene mutation of acute lymphoblastic leukemia (ALL) and an application method thereof. The detection kit includes a specific primer for an NT5C2 gene mutation site, which can comprehensively and accurately detect the high mutation site of an NT5C2 gene. The detection result has strong guiding significance for clinical practice, can be used for rapid and accurate detection of relapse and drug resistance of ALL to avoid unnecessary therapeutic drugs and is of great importance in the filed of detection and treatment of ALL. Through mutation detection of the NT5C2 gene related to ALL relapse and drug resistance, the detection kit can better guide the medication and treatment of clinicians, and can be used as an auxiliary means for diagnosis of relapse and drug resistance of the disease.

Description

technical field [0001] The invention belongs to the field of medicine and molecular detection, and specifically relates to a detection kit and application method for acute lymphoblastic leukemia (ALL) recurrence and drug-resistant gene mutation. Background technique [0002] Acute lymphoblastic leukemia (ALL) is a malignant tumor that originates from the abnormal proliferation of B-lineage or T-lineage blast cells of lymphocytes in the bone marrow. Abnormally proliferating blast cells can gather in the bone marrow and inhibit normal hematopoietic function, and can also invade other tissues outside the bone marrow, such as meninges, lymph nodes, gonads, liver, and bones. Bone marrow (BM) is involved in all ALL patients, peripheral blood (PB) is often involved in B-ALL, but T-ALL is more common. Involvement of extramedullary sites such as the central nervous system, lymph nodes, liver, spleen, and testes in men is also common. Clinically, dizziness, fatigue, palpitation, sho...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/106C12Q2600/118C12Q2600/156
Inventor 傅廷娇张娟程宇梁超王绪华陈忠黄士昂
Owner 北京海思特医学检验实验室有限公司
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