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A polycyclic aromatic hydrocarbon bioremediation agent, its preparation method and its application

A bioremediation agent and technology of polycyclic aromatic hydrocarbons, applied in the field of water pollution control, can solve the problem that only one or two types can be degraded at a time, and achieve the effect of improving biological treatment efficiency, high activity, and large biomass

Active Publication Date: 2021-08-17
NORTHWESTERN POLYTECHNICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention provides a polycyclic aromatic hydrocarbon bioremediation agent, which solves the problem that the polycyclic aromatic hydrocarbon bioremediation agent in the prior art can only degrade polycyclic aromatic hydrocarbons at about 30-38°C, and can only degrade one or two types of polycyclic aromatic hydrocarbons at a time. Aromatics problem

Method used

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  • A polycyclic aromatic hydrocarbon bioremediation agent, its preparation method and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A polycyclic aromatic hydrocarbon bioremediation agent, which is composed of the following components in parts by weight: 25 parts of Nocardia coliensis fermentation broth, 20 parts of Rhodopseudomonas paluscans fermentation broth, and 15 parts of Corkia palustris fermentation broth , Aspergillus nidulans fermentation broth 5 parts, peat 25 parts;

[0038] Among them, the number of effective viable bacteria in Nocardia coliensis fermentation liquid, Rhodopseudomonas palustris fermentation liquid, Cockerella palustris fermentation liquid and Aspergillus nidulans fermentation liquid was 10 8 -10 10cfu / mL.

[0039] Specifically follow the steps below:

[0040] Step 1, preparation of Nocardia coeliac fermentation broth

[0041] Adjust the pH value of the Nocardia liquid medium to 6-6.5, and inoculate the Nocardia liquid medium volume 5% of the Nocardia liquid medium volume therein, at 10-15°C, stir The speed is 300r / min, the ventilation volume is 0.30m 3 Cultivate for ...

Embodiment 2

[0057] A polycyclic aromatic hydrocarbon bioremediation agent, which is composed of the following components in parts by weight: 30 parts of Nocardia coliensis fermentation broth, 15 parts of Rhodopseudomonas paluscans fermentation broth, and 10 parts of Corkia palustris fermentation broth , Aspergillus nidulans fermentation broth 10 parts, peat 20 parts;

[0058] Among them, the number of effective viable bacteria in Nocardia coliensis fermentation liquid, Rhodopseudomonas palustris fermentation liquid, Cockerella palustris fermentation liquid and Aspergillus nidulans fermentation liquid was 10 8 -10 10 cfu / mL.

[0059] The preparation method is the same as that of Example 1, except that the formula is changed into that of Example 2.

Embodiment 3

[0061] A polycyclic aromatic hydrocarbon bioremediation agent, which is composed of the following components in parts by weight: 28 parts of Nocardia coliensis fermentation broth, 18 parts of Rhodopseudomonas paluscans fermentation broth, and 12 parts of Corkia palustris fermentation broth , 8 parts of Aspergillus nidulans fermentation liquid, 30 parts of peat;

[0062] Among them, the number of effective viable bacteria in Nocardia coliensis fermentation liquid, Rhodopseudomonas palustris fermentation liquid, Cockerella palustris fermentation liquid and Aspergillus nidulans fermentation liquid was 10 8 -10 10 cfu / mL.

[0063] The preparation method is the same as in Example 1, except that the formula is changed into that of Example 3.

[0064] In order to illustrate the effect, the present invention also provides a comparative example, specifically as follows.

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Abstract

The invention discloses a polycyclic aromatic hydrocarbon bioremediation agent, which is composed of the following components in parts by weight: 25-30 parts of Nocardia coliensis fermented liquid, 15-20 parts of Rhodopseudomonas paluscans fermented liquid, marsh cock 10-15 parts of A. nidulans fermentation broth, 5-10 parts of Aspergillus nidulans fermentation broth, and 20-30 parts of adsorption carrier. In addition, the invention also provides a preparation method of the polycyclic aromatic hydrocarbon bioremediation agent and its application in groundwater remediation of oil-contaminated sites. Aiming at polycyclic aromatic hydrocarbons in groundwater of oil-contaminated sites, the present invention prepares various strains capable of forming dominant flora into high-efficiency microbial preparations, accelerates the degradation of polycyclic aromatic hydrocarbons by microorganisms, improves the biological treatment efficiency of the system, and ensures stable operation of the system ; and the various strains of the present invention are reasonably compatible, symbiotically coordinated, non-antagonistic, high in activity, large in biomass, and fast in reproduction. When added to the groundwater of oil-contaminated sites, it has a good degradation effect on polycyclic aromatic hydrocarbons.

Description

technical field [0001] The invention belongs to the technical field of water pollution control, and in particular relates to a polycyclic aromatic hydrocarbon bioremediation agent, its preparation method and its application. Background technique [0002] Due to the carcinogenicity, mutagenicity, and teratogenicity of PAHs and their derivatives, as well as acute toxicity and sublethal effects, the remediation of PAHs in groundwater environments is currently a research hotspot in the field of environmental pollution remediation. Among them, the bioremediation technology of polycyclic aromatic hydrocarbons in the groundwater pollution caused by petroleum hydrocarbons is one of the focuses of international research. [0003] PAHs are widely present in crude oil and petroleum products. With the production and widespread use of petroleum and its products, the pollution of PAHs caused by various channels is becoming more and more serious. Among them, petroleum hydrocarbons directly...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34C12N1/20C12N1/14C12N11/14C12R1/01C12R1/38C12R1/66C02F103/06C02F101/32
CPCC02F3/34C02F2101/327C02F2103/06C12N1/14C12N1/20C12N11/14
Inventor 孙然钱进樊琳吴耀国胡思海产江为
Owner NORTHWESTERN POLYTECHNICAL UNIV
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