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Method for extracting saponin in plants through microbial fermentation

A microbial fermentation and saponin technology, applied in chemical instruments and methods, glycosides, glycosides, etc., can solve the problems of saponin yield and purity to be improved, and achieve the effect of increasing yield and simple operation

Inactive Publication Date: 2018-12-14
李晌
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the yield and purity of the saponins obtained by the above extraction and purification methods need to be improved (the yield is 15-25%, and the purity is 45-60%)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The method for extracting saponins in plants by microbial fermentation provided in this embodiment comprises the following steps:

[0027] (1) Take Sapinberry peel and saponin and dry it in an oven at 50°C, crush the dried Sapindus peel and saponin through a 1-mesh sieve, and take 1Kg of a mixture of Sapindia peel powder and saponin powder And put it into a 10L extractor, add 10Kg deionized water, heat and reflux extraction 3 times, each time reflux extraction for 2.5h, combine the 3 extractions, filter, and concentrate the filtrate to 350g to obtain solution A;

[0028] (2) Add 0.8 times the amount of deionized water 280mL to solution A, and let it stand at 0°C for 51 hours, filter, concentrate the filtrate to 400g, and put it at room temperature to obtain solution B;

[0029] (3) Add an aqueous ethanol solution with a concentration of 92v% to the solution B so that the concentration of the alcohol content is 75v%, and stand at 5°C for 40 hours, filter, and concentrate...

Embodiment 2

[0036] The method for extracting saponins in plants by microbial fermentation provided in this embodiment comprises the following steps:

[0037](1) Take the Sapindia fruit peel and saponin and dry it in an oven at 60°C, crush the dried Sapindil fruit and sapindosa through a 20-mesh sieve, and take 1Kg of the mixture of Sapindia sapindus peel powder and saponin powder And put it into a 10L extractor, add 5Kg deionized water, heat and reflux extraction twice, each time reflux extraction for 3.5h, combine the two extracts, filter, and concentrate the filtrate to 400g to obtain solution A;

[0038] (2) Add 0.5 times the amount of deionized water 200mL to solution A, and let it stand at 8°C for 60 hours, filter, concentrate the filtrate to 350g, and let it cool to room temperature to obtain solution B;

[0039] (3) Add an aqueous ethanol solution with a concentration of 82v% to the solution B so that the concentration of the alcohol content is 80v%, and let it stand at 2°C for 50 ...

Embodiment 3

[0046] The method for extracting saponins in plants by microbial fermentation provided in this embodiment comprises the following steps:

[0047] (1) Take the Sapindia fruit peel and saponin and dry it in an oven at 60°C, crush the dried Sapindil fruit and saponin through a 15-mesh sieve, and take 1Kg of the mixture And put it into a 10L extractor, add deionized water with a concentration of 7Kg, heat and reflux extraction twice, each time reflux extraction for 3.0h, combine the two extracts, filter, and concentrate the filtrate to 360g to obtain solution A;

[0048] (2) Add 0.7 times the amount of deionized water 252mL to solution A, and let it stand at 2°C for 55h, filter, concentrate the filtrate to 380g, and put it at room temperature to obtain solution B;

[0049] (3) Add an aqueous ethanol solution with a concentration of 90v% to the solution B so that the concentration of the alcohol content is 78v%, and stand at 4°C for 45h, filter, and concentrate the filtrate to 390g...

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Abstract

The invention provides a method for extracting saponin in plants through microbial fermentation. The method comprises the following steps: firstly, extracting saponin in sapindus mukurossi peel and Chinese honeylocust fruits by using deionized water, respectively removing impurities in an extracting solution by using deionized water and an ethanol solution, then degrading saccharides, proteins, pectin, cellulose and other substances in the extract by adopting microorganism yeast, and finally, further purifying the saponin in the extract by utilizing cation exchange resin and AB-8 macroporous adsorption resin in sequence, so that the saponin yield and the purity of the saponin in the extract can be improved to the maximum extent. In addition, due to the fact that the saponin shows certain weak alkalinity, the saponin can be salified and dissolved in an acid solution and can be precipitated under an alkaline condition. Therefore, the saponin is refined by adopting a hydrochloric acid ethanol-ammonia water precipitation method, and the preparation method is simple to operate, and is scientific and reasonable. The saponin extracted by the method of extracting saponin in plants throughmicrobial fermentation can be directly used in various cleaning agent products.

Description

technical field [0001] The invention relates to a method for extracting saponins from plants, in particular to a method for extracting saponins from plants by microbial fermentation. Background technique [0002] Soapberry, also known as hand-washing fruit or round soap, is distributed in vast areas such as Southeast Asian countries, and it has a wide range of applications in greening, soil and water conservation, and environmental protection. With the in-depth study of Sapindus chinensis, its chemical composition and pharmacological effects have been gradually discovered, which has attracted more and more attention. Sapindus saponin is mainly distributed in the skin of Sainberry, which is an excellent natural surfactant. Sapindus saponin not only has good emulsifying, dispersing, foaming, moisturizing effects, but also has bactericidal, analgesic, and anti-inflammatory properties. , insecticidal and blood pressure lowering effects. As a natural active substance, Sapindus ...

Claims

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Application Information

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IPC IPC(8): C07G3/00C11D1/66
CPCC07G3/00C11D1/662
Inventor 李晌
Owner 李晌
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