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Biological preparation capable of effectively increasing inosinic acid content of crucian carps and preparation method thereof

A technology of biological preparations and inosinic acid, applied in animal feed, animal feed, additional food elements, etc., can solve the problems of weak Brevibacterium ammoniagenes phosphatase activity and poor permeability of inosinic acid to cell membranes, etc., to enhance the body Immunity function, improving animal anti-stress, and low loss rate

Active Publication Date: 2018-12-18
SHANGHAI PROBIOTIK BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fermentation method can make inosine phosphorylation of Brevibacterium ammoniagenes to produce inosinic acid, but the permeability of inosinic acid to the cell membrane is very poor, and the phospholipase activity of Brevibacterium ammoniagenes is weak; inosinic acid is ubiquitous in microorganisms Dephosphorylated enzymes, the phospholipase of Bacillus subtilis has strong activity, which is conducive to the dephosphorylation of inosinic acid to form inosinic acid

Method used

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  • Biological preparation capable of effectively increasing inosinic acid content of crucian carps and preparation method thereof
  • Biological preparation capable of effectively increasing inosinic acid content of crucian carps and preparation method thereof
  • Biological preparation capable of effectively increasing inosinic acid content of crucian carps and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Step 1: Breeding and production of strains:

[0039] 1) Bacillus subtilis and Brevibacterium ammoniagenes provided by the Culture Preservation Center of the Ministry of Agriculture are used to subculture on nutrient agar medium, and Saccharomyces cerevisiae is subcultured on starch medium to select strains with fast growth and obvious colony characteristics , store strains at a temperature of 2°C;

[0040]Nutrient agar medium: beef extract 3g, peptone 10g, NaCL 5g, agar 15-20g, water 1000ml, PH7-7.2, sterilized at 121°C for 20min;

[0041] Starch medium: peptone 10g, NaCl 5g, beef extract 5g, soluble starch 2g, distilled water 1000ml, agar 15-20g, sterilized at 121°C for 20min;

[0042] 2) Transfer Brevibacterium ammoniagenes and Saccharomyces cerevisiae into eggplant bottles filled with nutrient agar medium, culture at 38°C for 45 hours, and store in a refrigerator at 4°C after the surface of the eggplant bottle is covered with a lawn .

[0043] Step 2, first stage ...

Embodiment 2

[0055] Step 1: Breeding and production of strains:

[0056] 1) Bacillus subtilis and Brevibacterium ammoniagenes provided by the Culture Preservation Center of the Ministry of Agriculture are used to subculture on nutrient agar medium, and Saccharomyces cerevisiae is subcultured on starch medium to select strains with fast growth and obvious colony characteristics , preserve the strains at a temperature of 44°C;

[0057] Nutrient agar medium: beef extract 3g, peptone 10g, NaCL 5g, agar 15-20g, water 1000ml, PH7-7.2, sterilized at 121°C for 20min;

[0058] Starch medium: peptone 10g, NaCl 5g, beef extract 5g, soluble starch 2g, distilled water 1000ml, agar 15-20g, sterilized at 121°C for 20min;

[0059] 2) Transfer Brevibacterium ammoniagenes and Saccharomyces cerevisiae into eggplant bottles filled with nutrient agar medium, culture at 38°C for 45 hours, and store in a refrigerator at 4°C after the surface of the eggplant bottle is covered with a lawn .

[0060] Step 2, fir...

Embodiment 3

[0072] The biological preparation provided by the invention is added to fish feed, and can improve the survival rate of crucian carp and the content of inosinic acid in crucian carp muscle. With the fish pond of 2000 crucian carp fingerlings of about 30 grams of body weight per mu as implementation object, the control group is fed with common fish feed, and the experimental group is fed with the fish feed that has added the biological preparation of the present invention. After 10 months, clear ponds are fished, Statistical yield per mu, average body weight and inosinic acid content in crucian carp muscle, the statistical results are as follows in Table 1:

[0073] Table 1 Statistics of production data fed with different fish feeds

[0074]

[0075] It can be seen from the above table that when the biological preparation of the present invention is added to fish feed, the survival rate and meat quality of crucian carp are far superior to those of the control group.

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Abstract

The invention provides a biological preparation capable of effectively increasing inosinic acid content of crucian carps. The biological preparation capable of effectively increasing the inosinic acidcontent of the crucian carps comprises the following raw materials in parts by weight: 10-50% of bacillus subtilis, 20-45% of brevibacterium ammoniagenes, 10-40% of beer yeast, 0-20% of zeolite powder, and 0-8% of cellulase. The invention further provides a preparation method of the biological preparation. According to the biological preparation and the preparation method thereof provided by theinvention, immunologic active substances in the biological preparation are capable of enhancing body immunologic functions so as to enhance anti-stress and anti-disease abilities of animals as well asimprove production yield of the animals; moreover, the biological preparation also has the advantages of being small in toxic and side effects, free of drug resistance, not liable to left residues and so on. In addition, the biological preparation is rich in nutrients, and enriched with a plurality of amino acids, proteins and trace elements; moreover, the biological preparation is rich in a plurality of organic acids and enzymes and enzymes, so that the biological preparation is capable of raising survival rate of crucian carps as well as effectively improving meat quality of the crucian carps.

Description

technical field [0001] The invention relates to the field of aquaculture microbial feed additives, in particular to a biological preparation for effectively increasing the content of crucian carp inosinic acid and a preparation method thereof. Background technique [0002] Crucian carp, an omnivorous fish, likes to live in groups and live by choosing food. The meat is tender and has high nutritional value. Every 100 grams of meat contains 13 grams of protein, 11 grams of fat, and contains a lot of calcium, phosphorus, iron and other minerals. Crucian carp has extremely high medicinal value. Its nature is flat and sweet, and it enters the stomach and kidney. Crucian carp is widely distributed and is produced all year round in waters all over the country. Crucian carp from February to April and August to December is the most plump and one of the important edible fish in my country. The maximum body length is about 30cm, and the inhabiting depth is 0-20 meters. It is non-toxi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/18A23K20/189A23K20/28A23K50/80
CPCA23K10/18A23K20/189A23K20/28A23K50/80Y02A40/818
Inventor 江旸
Owner SHANGHAI PROBIOTIK BIOLOGICAL TECH
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