A method for extracting lutein from marigold

A technology of lutein and marigold, which is applied in the direction of organic chemistry, can solve the problems of complex extraction process, high solvent toxicity, and low product purity, and achieve the effects of simple process, increased saponification rate, and reduced alkali consumption

Active Publication Date: 2020-07-31
CHAMBROAD CHEM IND RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention solves the existing problems of complex lutein extraction process, high solvent toxicity and low product purity, and has simple process, strong operability and high product purity, and is suitable for industrial scale production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Weigh 100g of marigold powder, add 1L of n-hexane, stir at room temperature, extract for 4h, filter with suction, and rotary evaporate the filtrate at 50°C to obtain lutein ester, add 500mL of 1% KOH ethanol solution (50% ethanol concentration) and 0.5g of enzyme activity 30kU / g The high-temperature alkaline lipase was saponified at 60°C for 30min, the saponified solution was left at 4°C for 12h, filtered with suction, the filter cake was washed with distilled water until neutral, and dried at 50°C to obtain 1.82g of lutein crystals with a purity of 76.84 %.

Embodiment 2

[0025] Weigh 100g of marigold powder, add 1L of petroleum ether, stir at room temperature, extract for 4h, filter with suction, and rotary evaporate the filtrate at 55°C to obtain lutein ester, add 400mL of 2% KOH ethanol solution (40% ethanol concentration) and 2g of lutein with enzyme activity of 5kU / g High-temperature alkaline lipase was saponified at 55°C for 2 hours, and the saponified solution was kept at -8°C for 10 hours, filtered with suction, and the filter cake was washed with distilled water until neutral, and dried at 60°C to obtain 1.75g ​​of lutein crystals with a purity of 85.83 %.

Embodiment 3

[0027] Weigh 100g of marigold powder and add 1.5L mixture of n-hexane, petroleum ether and ethanol (volume ratio 1:1:1) to extract at room temperature for 3h, filter with suction, and rotary evaporate the filtrate at 60°C to obtain lutein ester, add 500mL of 1% KOH Ethanol solution (60% ethanol concentration) and 1 g of high-temperature alkaline lipase with an enzyme activity of 20 kU / g were saponified at 65° C. for 1.5 h, and the saponified solution was left to stand at 0° C. for 16 h, filtered with suction, and the filter cake was rinsed with 50 mL of cold ethanol, and then Wash with distilled water until neutral, freeze-dry in vacuum at -50°C to obtain 1.90 g of lutein crystals with a purity of 76.98%.

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Abstract

The invention belongs to the field of plant purification, and provides a method for extracting lutein from marigolds. The method includes the following steps: (1) adding dry marigold powder and organic solvents into an extraction container for stirring and extracting; (2) filtering the mixture in the extraction container, collecting filtrate, and subjecting the filtrate to decompressed concentration to obtain a lutein ointment; (3) adding a potassium hydroxide ethanol solution and high-temperature alkaline lipases into the lutein ointment, and after stirring and dissolving, transferring the lutein ointment into a reactor for saponification; (4) standing saponification liquid at a low temperature to precipitate out lutein crystals, performing filtration, washing filter cakes by distilled water, and drying the filter cakes to obtain the high-purity lutein crystals, wherein the purity of the lutein in the final products ranges from 70% to 90%. The method has the advantages that the problems of complicated extraction process, high solvent toxicity and low product purity of existing lutein are solved; the method has a simple process, high operability and high product purity, and is suitable for industrial mass production.

Description

technical field [0001] The invention belongs to the technical field of natural plant purification and provides a method for extracting lutein from marigolds. Background technique [0002] Marigold is not only a beautiful ornamental flower, but also a kind of Chinese herbal medicine. Marigold dry powder contains 0.6% to 2.5% lutein. Therefore, marigold is an ideal raw material for extracting lutein. [0003] Lutein exists in the form of lauric acid, myristic acid and palmitate in marigold petals. Therefore, it needs to be extracted, saponified, separated and purified to prepare lutein. The main function of lutein is to protect eyesight. At the same time, it is also a natural antioxidant, which has the functions of anti-oxidation, improving immunity, preventing cancer and preventing arteriosclerosis. Lutein has been widely used in food, feed, medicine, cosmetics, health products and other fields. [0004] Existing extraction techniques for lutein include solvent extraction, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C403/24
CPCC07C403/24C07C2601/16
Inventor 冯文娟马韵升栾波李超孟魏圣可许晅徐泽平王秀芝虞凤慧孙建忠韩立霞
Owner CHAMBROAD CHEM IND RES INST CO LTD
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