A method for extracting lutein from marigold
A technology of lutein and marigold, which is applied in the direction of organic chemistry, can solve the problems of complex extraction process, high solvent toxicity, and low product purity, and achieve the effects of simple process, increased saponification rate, and reduced alkali consumption
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Embodiment 1
[0023] Weigh 100g of marigold powder, add 1L of n-hexane, stir at room temperature, extract for 4h, filter with suction, and rotary evaporate the filtrate at 50°C to obtain lutein ester, add 500mL of 1% KOH ethanol solution (50% ethanol concentration) and 0.5g of enzyme activity 30kU / g The high-temperature alkaline lipase was saponified at 60°C for 30min, the saponified solution was left at 4°C for 12h, filtered with suction, the filter cake was washed with distilled water until neutral, and dried at 50°C to obtain 1.82g of lutein crystals with a purity of 76.84 %.
Embodiment 2
[0025] Weigh 100g of marigold powder, add 1L of petroleum ether, stir at room temperature, extract for 4h, filter with suction, and rotary evaporate the filtrate at 55°C to obtain lutein ester, add 400mL of 2% KOH ethanol solution (40% ethanol concentration) and 2g of lutein with enzyme activity of 5kU / g High-temperature alkaline lipase was saponified at 55°C for 2 hours, and the saponified solution was kept at -8°C for 10 hours, filtered with suction, and the filter cake was washed with distilled water until neutral, and dried at 60°C to obtain 1.75g of lutein crystals with a purity of 85.83 %.
Embodiment 3
[0027] Weigh 100g of marigold powder and add 1.5L mixture of n-hexane, petroleum ether and ethanol (volume ratio 1:1:1) to extract at room temperature for 3h, filter with suction, and rotary evaporate the filtrate at 60°C to obtain lutein ester, add 500mL of 1% KOH Ethanol solution (60% ethanol concentration) and 1 g of high-temperature alkaline lipase with an enzyme activity of 20 kU / g were saponified at 65° C. for 1.5 h, and the saponified solution was left to stand at 0° C. for 16 h, filtered with suction, and the filter cake was rinsed with 50 mL of cold ethanol, and then Wash with distilled water until neutral, freeze-dry in vacuum at -50°C to obtain 1.90 g of lutein crystals with a purity of 76.98%.
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