Delayed-lasing luminescent nano probe and preparation method thereof

A nanoprobe and nanoparticle technology, applied in the fields of nanomaterials and biological detection, can solve problems such as short luminescence lifetime, achieve the effect of safe method, suitable for large-scale popularization and application, and improve detection accuracy

Active Publication Date: 2018-12-21
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such a short luminescence lifetime is disadvantageous for biological probes, since most of the luminescence signal will be masked by the strong spontaneous emission background

Method used

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  • Delayed-lasing luminescent nano probe and preparation method thereof
  • Delayed-lasing luminescent nano probe and preparation method thereof
  • Delayed-lasing luminescent nano probe and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Such as figure 1 As shown, the delayed lasing luminescence nanoprobe has a three-layer core-shell structure, including Au nanoparticles 4, a dense silica isolation layer 3 wrapped on the surface of the Au nanoparticles 4, and dye molecules 1 doped on the surface of the isolation layer. Silica shell 2. The preparation method of the delayed lasing luminescence nanoprobe is as follows:

[0031] (1) Preparation of silylating reagents for dye molecular chelation:

[0032] Take 470 μL of 4.56 mM dimethyl sulfoxide solution of 2′,7′-difluorofluorescein maleimide, add 20 μL of 3-mercaptopropyltriethoxysilane under the protection of nitrogen atmosphere, and react at room temperature for 30 ~40min to prepare the silylating reagent for dye molecular chelation.

[0033] (2) Prepare the dispersion of Au nanoparticles:

[0034] In a 50mL flask, add 20mL of a 0.01% mass fraction of chloroauric acid solution, add 0.60mL of a 1% sodium citrate solution after boiling, react under boi...

Embodiment 2

[0038] The preparation method of the delayed lasing luminescence nanoprobe is as follows:

[0039] (1) Preparation of silylating reagents for dye molecular chelation:

[0040] Take 470 μL of 4.56 mM dimethyl sulfoxide solution of 2′,7′-difluorofluorescein maleimide, add 25 μL of 3-aminopropyltriethoxysilane under the protection of nitrogen atmosphere, and react at room temperature for 40 minutes A silylating reagent for chelating dye molecules is prepared.

[0041] (2) Prepare the dispersion of Au nanoparticles:

[0042] In a 50mL flask, add 20mL of a 0.01% mass fraction of chloroauric acid solution, add 0.60mL of a 1% sodium citrate solution after boiling, react under boiling conditions for 30min and then cool to room temperature to obtain a dispersion of Au nanoparticles .

[0043] (3) Add 60 μL of 3-mercaptopropyltriethoxysilane to the dispersion of Au nanoparticles prepared in step (2), stir and react at room temperature for 20 min, then add 350 μL of a sodium silicate ...

Embodiment 3

[0046] The preparation method of the delayed lasing luminescence nanoprobe is as follows:

[0047] (1) Preparation of silylating reagents for dye molecular chelation:

[0048] Take 470 μL of 4.56 mM dimethyl sulfoxide solution of 2′,7′-difluorofluorescein maleimide, add 12 μL of 3-mercaptopropyltrimethoxysilane under the protection of nitrogen atmosphere, and react at room temperature for 40 minutes to prepare A silylating reagent for chelating dye molecules.

[0049] (2) Prepare the dispersion of Au nanoparticles:

[0050] In a 50mL flask, add 20mL of a 0.01% mass fraction of chloroauric acid solution, add 0.60mL of a 1% sodium citrate solution after boiling, react under boiling conditions for 30min and then cool to room temperature to obtain a dispersion of Au nanoparticles .

[0051] (3) Add 60 μL of 3-mercaptopropyltriethoxysilane to the dispersion of Au nanoparticles prepared in step (2), stir and react at room temperature for 20 min, then add 350 μL of a sodium silica...

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Abstract

The invention discloses a delayed-lasing luminescent nano probe which is prepared from Au nanoparticles, a compact silicon dioxide isolating layer and a dye molecule doped silicon dioxide layer, wherein the surfaces of the Au nanoparticles are coated by the compact silicon dioxide isolating layer, the surface of the dye molecule doped silicon dioxide layer is covered by the compact silicon dioxideisolating layer, the dye molecules have triple excited states to form a three-energy-level structure, and resonant frequencies of the Au nanoparticles are matched with the triple-state energy levelsof the dye molecules. The invention further discloses a preparation method of the luminescent nano probe. A plasma lasing spectral line width of the luminescent nano probe disclosed by the invention is only about 3 nm, a lasing threshold value is as low as 1.0 mJ/cm<2>, and the luminescence lifetime is about 102 mu s; the luminescent nano probe can be applied to simultaneously detecting a great many channels, the detection accuracy is improved, and the luminescent nano probe is sutiable for biological detection; furthermore, the preparation method of the luminescent nano probe has the advantages of safety, high efficiency and suitability for large-scale popularization and application.

Description

technical field [0001] The invention relates to the fields of nanomaterials and biological detection, in particular to a delayed laser luminescence nanoprobe and a preparation method thereof. Background technique [0002] So far, the wide application of luminescent probes such as fluorescent dyes and proteins, semiconductor nanocrystals (i.e., quantum dots), upconverting nanoparticles, and bioluminescent molecules in related fields such as life sciences, medicine, and chemical engineering has produced immeasurable results. Social value. However, traditional luminescent probes still have many disadvantages, such as wide emission spectrum range and short luminescence lifetime. Generally, fluorescent dyes and proteins have emission spectrum peak widths of about 50-100nm (Chem.Rev., 2012, 112, 4391-4420), while quantum dots and up-conversion nanoparticles have narrower spectra, but still 30-50nm (Chem. . Rev., 2016, 116, 10820-10851; Nat. Nanotechnol., 2015, 10, 924). The bro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/06C09K11/58G01N21/64B82Y30/00
CPCB82Y30/00C09K11/025C09K11/06C09K11/58C09K2211/1088G01N21/6428
Inventor 康斌宋沛徐静娟陈洪渊
Owner NANJING UNIV
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