Preparation method and application of photoelectrochemical N-terminal pro-brain natriuretic peptide sensor based on mesoporous flower-like tin oxide composite material

A photoelectrochemical and composite material technology, applied in the preparation and application of photoelectrochemical N-terminal forebrain natriuretic peptide sensors based on mesoporous flower-like tin oxide composite materials, can solve the problems of cumbersome steps, low sensitivity, and long detection cycle , to achieve high surface activity, enhanced sensitivity, and improved photoelectric performance

Active Publication Date: 2018-12-21
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the N-terminal N-terminal N-terminal natriuretic peptide can be determined by enzyme-linked immunosorbent assay, electrochemiluminescence and other methods, but because of the influence of many factors on its quantitative determination, it has the disadvantages of low sensitivity, long detection period and cumbersome steps.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Preparation of SnO 2 / NCQDs / Bi 2 S 3 The specific steps are:

[0033] (1) Add 0.01 g of thioacetamide to 10 mL of isopropanol, stir until the thioacetamide is dissolved, then add 7 μL of tin chloride, stir to mix evenly and the solution is clear, transfer the solution to 50 mL In the reaction kettle, place it in a blast drying oven at 180 °C to react for 24 h. After the reaction is completed, the obtained product is washed with ultrapure water and ethanol for 3 times, and dried at 80 °C for 12 h to obtain SnO 2 powder, transfer the powder to a muffle furnace, calcinate at 500°C for 0.5 h, cool to room temperature, collect the powder for grinding, and obtain calcined SnO 2 , the calcined SnO 2 The powder was dispersed in ultrapure water and ultrasonicated for 20 s to obtain SnO 2 suspension;

[0034] (2) Cut the ITO electrode to a size of 2.5 cm × 0.8 cm, ultrasonically clean it with acetone, ethanol and ultrapure water for 30 min, and dry it in an oven a...

Embodiment 2

[0036] Embodiment 2 prepares SnO 2 / NCQDs / Bi 2 S 3 The specific steps are:

[0037] (1) Add 0.02 g of thioacetamide to 20 mL of isopropanol, stir until the thioacetamide is dissolved, then add 14 μL of tin chloride, stir to make it evenly mixed and the solution is clear, transfer the solution to 50 mL In the reaction kettle, place it in a blast drying oven at 180 °C to react for 24 h. After the reaction is completed, the obtained product is washed with ultrapure water and ethanol for 3 times, and dried at 80 °C for 12 h to obtain SnO 2 powder, transfer the powder to a muffle furnace, calcinate at 500°C for 1 h, and collect the powder after cooling to room temperature for grinding to obtain calcined SnO 2 , the calcined SnO 2 The powder was dispersed in ultrapure water and ultrasonicated for 20 s to obtain SnO 2 suspension;

[0038] (2) Cut the ITO electrode to a size of 2.5 cm × 0.8 cm, ultrasonically clean it with acetone, ethanol and ultrapure water for 30 min, and dry...

Embodiment 3

[0040] Embodiment 3 prepares SnO 2 / NCQDs / Bi 2 S 3 The specific steps are:

[0041] (1) Add 0.01 g of thioacetamide to 10 mL of isopropanol, stir until the thioacetamide is dissolved, then add 7 μL of tin chloride, stir to mix evenly and the solution is clear, transfer the solution to 50 mL In the reaction kettle, place it in a blast drying oven at 180 °C to react for 24 h. After the reaction is completed, the obtained product is washed with ultrapure water and ethanol for 3 times, and dried at 80 °C for 12 h to obtain SnO 2 powder, transfer the powder to a muffle furnace, calcinate at 500 °C for 2 h, and collect the powder after cooling to room temperature for grinding to obtain calcined SnO 2 , the calcined SnO 2 The powder was dispersed in ultrapure water and ultrasonicated for 20 s to obtain SnO 2 suspension;

[0042] (2) Cut the ITO electrode to a size of 2.5 cm × 0.8 cm, ultrasonically clean it with acetone, ethanol and ultrapure water for 30 min, and dry it in an ...

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PUM

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Abstract

The invention relates to a preparation method and application of a photoelectrochemical N-terminal pro-brain natriuretic peptide sensor based on a mesoporous flower-like tin oxide composite material,and belongs to the field of photoelectrochemical sensors. The novel flower-like SnO2 synthesized by hydrothermal method has a mesoporous structure for a better storage space, and a larger specific surface area and higher surface activity are beneficial for growth of smaller-sized nanoparticles on the surface. Sensitizing SnO2 with nitrogen-doped carbon quantum dots NCQDs can enhance absorption ofvisible light. Bi2S3 nanoparticles are grown in situ to obtain the mesoporous flower-like tin oxide composite material SnO2 / NCQDs / Bi2S3 with significantly improved photoelectric activity. N-terminal pro-brain natriuretic peptide antibody, bovine serum albumin and N-terminal pro-brain natriuretic peptide antigen are assembled onto the SnO2 / NCQDs / Bi2S3 composite material by a layer-by-layer self-assembly method. Ultra-sensitive detection of the N-terminal pro-brain natriuretic peptide is realized by utilizing the excellent photoelectric activity of SnO2 / NCQDs / Bi2S3 and the specific binding of N-terminal pro-brain natriuretic peptide antigen and antibody, which is important for analysis and detection of N-terminal pro-brain natriuretic peptide.

Description

technical field [0001] The invention relates to a preparation method and application of a photoelectrochemical N-terminal forebrain natriuretic peptide sensor based on a mesoporous flower-shaped tin oxide composite material. The electrochemical workstation is used for testing with a three-electrode system, and an LED lamp is used as a photoelectrochemical luminescence signal source. , flower-like SnO with mesoporous structure synthesized by hydrothermal method 2 , using nitrogen-doped carbon quantum dots NCQDs to sensitize SnO 2 , enhance its visible light absorption, and then grow Bi in situ 2 S 3 Nanoparticles to obtain mesoporous flower-like tin oxide composite SnO with significantly improved photoelectric activity 2 / NCQDs / Bi 2 S 3 , with SnO 2 / NCQDs / Bi 2 S 3 As a base material, a photoelectrochemical N-terminal forebrain natriuretic peptide sensor based on a mesoporous flower-like tin oxide composite material is prepared, which belongs to the field of photoelectr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/26G01N21/63
CPCG01N21/63G01N27/26G01N27/30
Inventor 范大伟鲍春竹刘昕张勇马洪敏吴丹王欢魏琴杜斌胡丽华
Owner UNIV OF JINAN
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