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Composite chromatography filtration membrane containing adsorption medium and preparation method and application of composite chromatography filtration membrane

A technology of adsorption medium and filter membrane, which is applied in the field of composite chromatographic filter membrane and its preparation, can solve the problems of low grafting rate, low dynamic load, large floor area, etc., and achieve high flux, high surface area, uniform The effect of flow distribution

Inactive Publication Date: 2018-12-25
杭州九龄科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In view of the above deficiencies, the present invention provides a composite chromatographic filtration membrane containing an adsorption medium and its preparation method and application, which solves the problem of slow separation speed of chromatographic packing, low dynamic load, high cost, large floor area; grafting rate Low, few product types, etc.

Method used

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  • Composite chromatography filtration membrane containing adsorption medium and preparation method and application of composite chromatography filtration membrane
  • Composite chromatography filtration membrane containing adsorption medium and preparation method and application of composite chromatography filtration membrane
  • Composite chromatography filtration membrane containing adsorption medium and preparation method and application of composite chromatography filtration membrane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Composite coating C18 silica gel chromatography medium

[0038] After thermally compounding a polyethersulfone microporous filter membrane with a thickness of 120um and a membrane pore size of 0.45um and a polyester non-woven fabric with a thickness of 150um, it is cut into a 20×20cm membrane, which is completely wetted with RO water , The immersion time is half an hour, the pores of the membrane are fully filled, and the water is squeezed out with double sticks; commercially available reversed-phase C18 silica gel filler with a particle size of 2-10um and a carbon loading of 10-12%. Prepared into ethanol gel solution, evenly coated on the composite filter membrane, the coating layer is a polyester non-woven fabric layer, the coating weight is 50g / ㎡, soak it in the absolute ethanol solution containing epichlorohydrin , The mass fraction ratio of epichlorohydrin in the absolute ethanol solution is 5%; after reacting at room temperature for 2 hours, the membrane i...

Embodiment 2

[0039] Example 2: Composite coating reversed-phase polymer chromatography medium, the surface group is phenyl

[0040] After thermally compounding a polyethersulfone microporous filter membrane with a thickness of 120um and a membrane pore size of 0.45um and a polyester non-woven fabric with a thickness of 150um, it is cut into a 20×20cm membrane, which is completely wetted with RO water , The immersion time is half an hour, the pores of the membrane are fully filled, and the water is squeezed out with double sticks; commercially available reversed-phase polymer chromatography packing, the matrix is ​​polymethyl methacrylate, and the surface group contains phenyl groups. The particle size is 3-10um, and the chromatographic medium has a pore size of 300-500 angstroms. It is formulated into an ethanol gel solution and evenly coated on the composite filter membrane. The coating layer is a polyester non-woven fabric layer, and the coating weight is 60g / ㎡; prepare 1000 ml of N-methylo...

Embodiment 3

[0041] Example 3: Composite coating hydrophobic chromatography medium, the surface group is butyl

[0042] After thermally compounding a polyethersulfone microporous filter membrane with a thickness of 120um and a membrane pore size of 5um and a polypropylene non-woven fabric with a thickness of 200um and a pore size of 100um, they are cut into 20×20cm membranes and complete with RO water. After soaking, wetting, soaking for half an hour, the pores of the membrane are fully filled, and the water is squeezed out with double sticks; a commercially available hydrophobic chromatography filler, the matrix is ​​polymethyl methacrylate, and the surface group contains butyl , The particle size is 15um, and the chromatographic medium has a pore size of 1000 angstroms. It is formulated into an ethanol gel solution and evenly coated on the composite filter membrane. The coating layer is polypropylene non-woven fabric layer, and the coating weight is 60g / ㎡ ; Prepare 1000 ml of N-methylol acr...

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Abstract

The invention discloses a composite chromatography filtration membrane containing an adsorption medium and a preparation method and application of the composite chromatography filtration membrane andrelates to the field of chromatography separation of membranes. The composite chromatography filtration membrane comprises a porous supporting layer and a filtration membrane body composited on the porous supporting layer, a functional layer formed by a chromatography medium is cross-linked with the outer side of the porous supporting layer, and the obtained composite chromatography filtration membrane is faster in velocity, higher biding efficiency and better dynamic loading as compared with a traditional chromatography medium, and the composite chromatography filtration membrane is excellentin reproducibility, convenient in use, low in cost and less amount of a buffer used; diffusion limitation of packing is avoided due to the opening-type membrane pore structure, biomolecules can be transported directly to junctions by fluid action, high dynamic binding loads can be achieved within a wide range of velocities and molecular ranges, and steep elution peak shapes are formed; macromolecules are captured or removed at high velocities, advantages of the packing and the filter membrane are combined, and significant advantages over conventional chromatography packing are achieved.

Description

Technical field [0001] The invention relates to the technical field of separation and purification of biological products, in particular to a composite chromatography filter membrane containing an adsorption medium, and a preparation method and application thereof. Background technique [0002] Large-scale and economical protein purification has increasingly become an important issue in the biotechnology industry. Generally speaking, protein is produced by cell culture, and the eukaryotic or prokaryotic production of the protein is modified by inserting a recombinant plasmid containing the gene of the protein of interest. Cell lines, because the cells usually used are living organisms, they must be fed with a complex growth medium containing sugars, amino acids and production factors, and the desired protein and the compound mixture fed into the cells and the cells themselves The separation of the by-products to a purity sufficient to be economically used as a human treatment pre...

Claims

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Application Information

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IPC IPC(8): B01D69/02B01D69/10B01D69/12B01D67/00B01D39/08B01D15/08B01D15/26B01D15/36B01D15/38
CPCB01D15/08B01D15/265B01D15/361B01D15/3804B01D39/08B01D67/0093B01D69/02B01D69/10B01D69/125B01D2239/0407B01D2239/0414B01D2239/0428B01D2239/0654B01D2239/10B01D2325/12B01D2325/38B01D2325/42
Inventor 刘锋江志波江润生唐勇红郭爱连
Owner 杭州九龄科技有限公司
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