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A kind of preparation method of wool keratin cortex cell

A technology of wool keratin and cells, which is applied in the field of preparation of wool keratin cortex cells, can solve problems such as toughening with L-polylactic acid film, and achieve the effect of strong directional degradation, simple method, and moderate effect

Active Publication Date: 2020-04-07
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the wool fiber diameter is as high as 20 μm, which is difficult to be used for the toughening of L-PLA film

Method used

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  • A kind of preparation method of wool keratin cortex cell
  • A kind of preparation method of wool keratin cortex cell
  • A kind of preparation method of wool keratin cortex cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Embodiment 1: a kind of preparation method of wool keratin cortex cell, the steps are as follows:

[0057] Step 1. Use calcium hypochlorite / hydrogen peroxide to remove the wool surface scale layer:

[0058] (1) Soak the waste rough wool in water at 25°C, the solid-to-liquid ratio is 1:25, soak the wool thoroughly, and add calcium hypochlorite Ca(ClO) with an active chlorine content of 70% 2 ·3H 2 O, standing for 5 minutes to obtain system A; wherein the amount of calcium hypochlorite added is 5% of the wool quality.

[0059] (2) slowly adding mass fraction in system A is 30% hydrogen peroxide, sodium pyrophosphate as peroxide stabilizer, ethylenediaminetetraacetic acid as peroxide chelating agent to form system B; Treat under constant temperature conditions for 20 minutes to make the wool react with hypochlorite and peroxide; wherein, based on the volume of the solution in system A, the amount of hydrogen peroxide with a mass fraction of 30% is 14ml / L, and the coke T...

Embodiment 2

[0069] Embodiment 2: a kind of preparation method of wool keratin cortex cell, the steps are as follows:

[0070] Step 1. Use calcium hypochlorite / hydrogen peroxide to remove the wool surface scale layer:

[0071] (1) Soak the waste fine wool in water at 20°C, the solid-to-liquid ratio is 1:20, soak the wool thoroughly, and add calcium hypochlorite Ca(ClO) with an active chlorine content of 70% 2 ·3H 2 O, stand still for 5 minutes to obtain system A; wherein the added amount of calcium hypochlorite is 3% of the wool quality.

[0072] (2) Slowly add hydrogen peroxide, sodium pyrophosphate as a peroxide stabilizer, and ethylenediaminetetraacetic acid as a peroxide chelating agent in system A to form system B; Treat under constant temperature conditions for 20 minutes to make wool react with hypochlorite and peroxide; wherein, based on the volume of the solution in system A, the mass fraction of 30% hydrogen peroxide is added in an amount of 10ml / L, The added amount of sodium ...

Embodiment 3

[0093] Embodiment 3: A method for preparing porous materials based on wool keratin cortex cells, the specific steps are as follows:

[0094] Step 1, using wool keratin cortex cells as raw materials, preparing an aqueous suspension of cortex cells with a mass percentage of 5%;

[0095] Step 2: Add glutaraldehyde to the aqueous suspension of cortical cells, the quality of glutaraldehyde in the mixed solution is respectively 0.15%, 0.8% and 1.5% of the mass of wool keratin cortical cells, and magnetically stir for 10 minutes to obtain three homogeneous milky white suspension;

[0096] Step 3. Quickly freeze the milky white suspension at -40°C for 10 hours, and then vacuum freeze-dry for 36 hours;

[0097] Step 4: Put the vacuum freeze-dried sample in an oven at 180° C. for 2 hours, and cross-link keratin with glutaraldehyde to obtain three kinds of porous materials of wool cortex cells.

[0098] Carry out following analysis for the experimental result of embodiment 3:

[0099]...

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Abstract

The invention provides a wool keratin cortex cell preparation method, and belongs to the field of wool keratin cortex cell extraction. The method includes the steps: firstly, pretreating wool by calcium hypochlorite / hydrogen peroxide and stripping wool surface scale layers; secondly, taking L-cysteine as a reducing agent, performing trypsin catalysis and degrading wool fibers by CMC (carboxymethylcellulose) to separate wool cortex cells; thirdly, further separating the cortex cells by ultrasonic treatment; finally, filtering and sucking filtrate to obtain the cortex cells and freezing and drying the cortex cells to obtain wool keratin cortex cells. The method is efficient and environmentally friendly.

Description

technical field [0001] The invention belongs to the field of extracting wool keratin cortex cells, and in particular relates to a preparation method of wool keratin cortex cells. Background technique [0002] With the continuous development of society, people's demand for environmentally friendly materials is increasing. In recent years, renewable and degradable natural polymer materials have received extensive attention. Waste wool keratin is a typical type of highly cross-linked protein, which has the advantages of good water resistance and stability, small thermal conductivity (good heat insulation performance), sound insulation, non-combustibility, and good elasticity. About 1 million tons of waste wool keratin is produced in my country every year, most of which are landfilled and not fully utilized. Waste wool keratin has the following main sources: (1) wool textile consumption field; (2) wool textile processing field: various waste 100% wool fibers and recycled silk ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/071C08L67/04C08L89/00C08J5/18
CPCC08J5/18C08J2367/04C08J2489/00C12N5/0627C12N2509/00
Inventor 侯秀良魏世锦林圣铭黄晓华马博谋徐荷澜
Owner JIANGNAN UNIV
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