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Discrete type biological sensing detection method for dangerous chemicals in baby bottle

A technology for hazardous chemicals and detection methods, applied in the field of discrete biosensor detection of hazardous chemicals in baby bottles, can solve the problems of high cost, poor stability, large amount of consumables, etc., achieve signal increase, improve detection sensitivity, and save reagents The effect of consumption

Inactive Publication Date: 2018-12-25
NINGBO HEIGER ELECTRICAL APPLIANCES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a discrete biosensing detection method for hazardous chemicals in baby feeding bottles, which aims to solve the problems of poor specificity, poor stability, large amount of consumables, and high cost in existing rapid detection technologies for hazardous chemicals. The detection limit of hazardous chemicals is greatly improved. This method has the advantages of generality, high stability, high sensitivity, and simple operation, and meets the detection requirements of various hazardous chemicals in milk bottles.

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  • Discrete type biological sensing detection method for dangerous chemicals in baby bottle
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  • Discrete type biological sensing detection method for dangerous chemicals in baby bottle

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Embodiment Construction

[0036] In order to enable those skilled in the art to better understand the solution of the present invention, the technical solution of the present invention will be further described below in conjunction with specific examples.

[0037] Below in conjunction with specific embodiment the present invention is described in detail

[0038] The specific example takes bisphenol A as an example, and the specific operation method is as follows:

[0039] Preparation of functionalized nanomaterials

[0040] (1) Preparation of up-conversion fluorescent nanomaterials:

[0041] Weigh YCl 3 ·6H 2 O 0.236g, YbCl 3 ·6H 2 O 0.077g, ErCl 3 ·6H 2 O 0.007g (Ln: 78% Y 3+ , 20%Yb 3+ , 2% Er 3+ ; a total of 1mmol) into a 100mL three-necked flask, add 4mL OA and 15mL 1-ODE, 1500r / min magnetic stirring while gradually warming up to 130 ° C, nitrogen protection to remove water vapor therein, further warming up to 160 ° C, to the mixture Cool down to room temperature naturally after forming ...

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Abstract

The invention discloses a discrete type biological sensing detection method for dangerous chemicals in a baby bottle, comprising the following steps of: modifying BPA aptamer (DNA1) and theirs complementary strands (DNA2) on the surface of AuNPs and UCNPs materials separately through covalent bonding, and preparing functionalized modified AuNPs, UCNPs probes (AuNPs-DNA1, UCNPs-DNA2). The detectionmethod of the dangerous chemicals whose UCNPs fluorescence is quenched by gold nanoparticles is constructed based on the fluorescence resonance energy transfer principle, realizes high sensitivity detection of a biological sensor on the dangerous chemicals, and is high in stability, repeatability and sensitivity.

Description

technical field [0001] The invention belongs to the technical field of detection and relates to a discrete biosensor detection method for hazardous chemicals in baby feeding bottles. Background technique [0002] Feeding bottles are now a commonly used breastfeeding tool in the family, but they can also cause health problems. Hazardous chemicals are invisible killers hidden in baby feeding bottles. There are many kinds of dangerous chemicals in baby feeding bottles, such as additives such as resins and stabilizers, plasticizers, and colorants. The bisphenol A incident that has occurred in recent years has aroused widespread concern about the hazardous chemicals in baby feeding bottles. Bisphenol A is the main raw material for the manufacture of epoxy resins, polycarbonate resins, and polystyrene resins. It is also used in phenolic resins, Stabilizer for polyvinyl chloride, antioxidant, etc. During breast-feeding, bisphenol A is likely to migrate out of the feeding bottle a...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6428G01N2021/6432
Inventor 丁利许宙康震清江王季诚李明安
Owner NINGBO HEIGER ELECTRICAL APPLIANCES
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